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Molecular cloning and immunolocalization of the 17 kDa myoglobin of Clonorchis sinensis

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Abstract

We purified the 17 kDa protein abundant in Clonorchis sinensis crude extracts. The N-terminal amino acid sequence of this protein was determined and an oligonucleotide probe synthesized. Using this probe, the cDNA encoding the protein was cloned and sequenced from the C. sinensis cDNA library. It was found to consist of a total of 150 amino acids and to have 41% conserved homology with the myoglobin of the trematodes Paramphistomum epiclitum and Isoparorchis hypselobagri. The gene product over-expressed in the bacterial system was purified and identified as the same molecule in the adult worms. BALB/c mouse sera raised against the adult 17 kDa protein revealed that this myoglobin was distributed throughout the parenchymal tissues except for the eggs and reproductive organs and that the protein may be involved in the survival of C. sinensis in the oxygen-depleted environment of the host.

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Acknowledgement

This work was supported by the Grant for the Reform of University Education under the BK21 Project of Seoul National University in 2000.

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Authors and Affiliations

  1. Department of Parasitology, College of Medicine, Cheju National University, Jeju 690-756, Korea

    Young-Bae Chung

  2. Department of Parasitology, College of Medicine, Ewha Woman's University, Seoul 158-710, Korea

    Hyun-Jong Yang

  3. Department of Parasitology, Chung-Ang University College of Medicine, Seoul 156-756, Korea

    Sung-Jong Hong & Shin-Yong Kang

  4. Department of Parasitology, Seoul National University College of Medicine, Seoul 110-799, Korea

    Mejeong Lee, Tae Yun Kim, Min-Ho Choi, Jong-Yil Chai & Sung-Tae Hong

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  1. Young-Bae Chung
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  2. Hyun-Jong Yang
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  4. Shin-Yong Kang
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  7. Min-Ho Choi
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  9. Sung-Tae Hong
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Correspondence to Min-Ho Choi.

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Chung, YB., Yang, HJ., Hong, SJ. et al. Molecular cloning and immunolocalization of the 17 kDa myoglobin of Clonorchis sinensis . Parasitol Res 90, 365–368 (2003). https://doi.org/10.1007/s00436-003-0837-2

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  • DOI: https://doi.org/10.1007/s00436-003-0837-2

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