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Ablation and analysis of small cell populations and single cells by consecutive laser pulses

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Abstract

Laser ablation of single cells through a sharpened optical fiber is used for the detection of metabolites by laser ablation electrospray ionization (LAESI) mass spectrometry (MS). Ablation of the same Allium cepa epidermal cell by consecutive pulses indicates the rupture of the cell wall by the second shot. Intracellular sucrose heterogeneity is detected by subsequent laser pulses pointing to rupturing the vacuolar membrane by the third exposure. Ion production by bursts of laser pulses shows that the drying of ruptured A. cepa cells occurs in ∼50 s at low pulse rates (10 pulses/s bursts) and significantly faster at high pulse rates (100 pulses/s bursts). These results point to the competing role of cytoplasm ejection and evaporative drying in diminishing the LAESI-MS signal in ∼50 s or 100 laser pulses, whichever occurs first.

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Authors and Affiliations

  1. Department of Chemistry, George Washington University, 725 21st Street, NW, Washington, DC, 20052, USA

    Bindesh Shrestha, Peter Nemes & Akos Vertes

Authors
  1. Bindesh Shrestha
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  2. Peter Nemes
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  3. Akos Vertes
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Correspondence to Akos Vertes.

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Paper presented at the 10th International Conference on Laser Ablation, 2009, Singapore.

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Shrestha, B., Nemes, P. & Vertes, A. Ablation and analysis of small cell populations and single cells by consecutive laser pulses. Appl. Phys. A 101, 121–126 (2010). https://doi.org/10.1007/s00339-010-5781-2

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  • DOI: https://doi.org/10.1007/s00339-010-5781-2

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