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Hym1p affects cell cycle progression in Saccharomyces cerevisiae

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Abstract

The Saccharomyces cerevisiae HYM1 gene is conserved among eukaryotes. The mammalian orthologue (called MO25) mediates signaling through the AMP-activated protein kinase and other related kinases, implicated in cell proliferation. In yeast, Hym1p plays a role in cellular morphogenesis and also promotes the daughter cell-specific localization of the Ace2p transcription factor. Here, we report that increased dosage of HYM1 apparently shortens the G1 phase of the cell cycle. In the absence of HYM1 or ACE2, mother and daughter cells divide with the same generation times. Genetic analysis of HYM1, ACE2 and CLN3 mutants suggests that these genes together contribute to the establishment of asynchronous mother–daughter cell divisions, but probably not in a linear pathway. Our overall data suggest that Hym1p has a regulatory role in cell cycle progression.

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Acknowledgements

We thank J. Miller for flow cytometry and thank F. Cross and B. Futcher for reagents. This work was supported by grants from the National Institutes of Health to M.P. (GM062377) and R.A. (GM058770).

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Authors and Affiliations

  1. Department of Biochemistry and Biophysics, Texas A&M University, 2128 TAMU, College Station, TX 77843, USA

    Lydia M. Bogomolnaya, Ritu Pathak, Jinbai Guo, Roxhana Cham & Michael Polymenis

  2. Department of Biology, Texas A&M University, 2128 TAMU, College Station, TX 77843, USA

    Rodolfo Aramayo

Authors
  1. Lydia M. Bogomolnaya
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  2. Ritu Pathak
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  3. Jinbai Guo
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  4. Roxhana Cham
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  5. Rodolfo Aramayo
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  6. Michael Polymenis
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Correspondence to Michael Polymenis.

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Communicated by S. Hohmann

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Bogomolnaya, L.M., Pathak, R., Guo, J. et al. Hym1p affects cell cycle progression in Saccharomyces cerevisiae. Curr Genet 46, 183–192 (2004). https://doi.org/10.1007/s00294-004-0527-3

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  • DOI: https://doi.org/10.1007/s00294-004-0527-3

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