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Species Diversity and Substrate Utilization Patterns of Thermophilic Bacterial Communities in Hot Aerobic Poultry and Cattle Manure Composts

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Abstract

This study investigated the species diversity and substrate utilization patterns of culturable thermophilic bacterial communities in hot aerobic poultry and cattle manure composts by coupling 16S rDNA analysis with Biolog data. Based on the phylogenetic relationships of 16S rDNA sequences, 34 thermophilic (grown at 60°C) bacteria isolated during aerobic composting of poultry manure and cattle manure were classified as Bacillus licheniformis, B. atrophaeus, Geobacillus stearothermophilus, G. thermodenitrificans, Brevibacillus thermoruber, Ureibacillus terrenus, U. thermosphaericus, and Paenibacillus cookii. In this study, B. atrophaeus, Br. thermoruber, and P. cookii were recorded for the first time in hot compost. Physiological profiles of these bacteria, obtained from the Biolog Gram-positive (GP) microplate system, were subjected to principal component analysis (PCA). All isolates were categorized into eight different PCA groups based on their substrate utilization patterns. The bacterial community from poultry manure compost comprised more divergent species (21 isolates, seven species) and utilized more diverse substrates (eight PCA groups) than that from cattle manure compost (13 isolates, five species, and four PCA groups). Many thermophilic bacteria isolated in this study could use a variety of carboxylic acids. Isolate B110 (from poultry manure compost), which is 97.6% similar to U. terrenus in its 16S rDNA sequence, possesses particularly high activity in utilizing a broad spectrum of substrates. This isolate may have potential applications in industry.

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Authors and Affiliations

  1. Graduate Institute of Veterinary Microbiology, National Chung Hsing University, Taichung, 40227, Taiwan, Republic of China

    Chao-Min Wang, Ching-Lin Shyu & Shiow-Her Chiou

  2. Department of Veterinary Medicine, National Chung Hsing University, Taichung, 40227, Taiwan, Republic of China

    Ching-Lin Shyu & Shu-Peng Ho

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  1. Chao-Min Wang
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  2. Ching-Lin Shyu
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  4. Shiow-Her Chiou
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Correspondence to Shiow-Her Chiou.

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The first two authors contributed equally to this work.

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Wang, CM., Shyu, CL., Ho, SP. et al. Species Diversity and Substrate Utilization Patterns of Thermophilic Bacterial Communities in Hot Aerobic Poultry and Cattle Manure Composts. Microb Ecol 54, 1–9 (2007). https://doi.org/10.1007/s00248-006-9139-4

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  • DOI: https://doi.org/10.1007/s00248-006-9139-4

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