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Protein oxidation and lipid peroxidation after renal ischemia-reperfusion injury: protective effects of erdosteine and N-acetylcysteine

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Abstract

Oxygen radicals have roles in the renal ischemia-reperfusion (IR) injury usually encountered in several conditions such as renal transplantation. The aim of this study was to investigate the effects of erdosteine and N-acetylcysteine (NAC) on the oxidant/antioxidant status and microscopy of renal tissues after IR injury. Male Sprague–Dawley rats were randomly assigned to four groups: control untreated rats, IR (30 min ischemia and 120 min reperfusion), IR + NAC (i.p.; 180 mg/kg) and IR + erdosteine (oral; 50 mg/kg/day for 2 days before experiments) groups. After unilateral renal IR, the right kidney was rapidly excised and sectioned vertically into two pieces for microscopic examination and biochemical analysis. Erdosteine and NAC treatment did not cause any significant change in the activity of superoxide dismutase (SOD) in comparison with the IR group, even if the SOD activity increased in IR groups than in the control group. Catalase (CAT) activity was decreased in the IR group in comparison with control and IR + erdosteine groups (P<0.05), whereas it was higher in the IR + erdosteine group than in the IR + NAC group (P<0.05). Xanthine oxidase (XO) activity was higher in all the IR-performed groups than in the control group (P<0.05). Thiobarbituric acid-reactive substances (TBARS) level and protein carbonyl (PC) content were increased after IR injury (P<0.05). Erdosteine or NAC treatments ameliorated these increased TBARS and PC contents in comparison with the IR group (P<0.05). Light microscopy of the IR group showed tubular dilatation, tubular necrosis and vacuole formation in epithelial cells. Erdosteine but not NAC apparently reduced the renal tissue damage. The pathological damage score after IR was significantly reduced after erdosteine treatment (P<0.05), but not after NAC treatment. In conclusion, renal IR resulted in oxidative damage as seen in biochemical lipid peroxidation and protein oxidation results with aggravated tubular necrosis. Erdosteine and NAC treatments improved the biochemical results of IR injury. However, on microscopic evaulations, animals receiving erdosteine showed a great reduction in renal damage when compared with the NAC group.

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References

  1. Rabb H, Daniels F, O’Donnell M, Haq M, Saba SR, Keane W, Tang WW (2000) Pathophysiological role of T lymphocytes in renal ischemia-reperfusion injury in mice. Am J Physiol Renal Physiol 279:F525–F531

    PubMed  CAS  Google Scholar 

  2. Dobashi K, Singh I, Orak JK, Asayama K, Singh AK (2002) Combination therapy of N-acetylcysteine, sodium nitroprusside and phosphoramidon attenuates ischemia-reperfusion injury in rat kidney. Mol Cell Biochem 240:9–17

    Article  PubMed  CAS  Google Scholar 

  3. Irmak MK, Koltuksuz U, Kutlu NO, Yagmurca M, Ozyurt H, Karaman A, Akyol O (2001) The effect of caffeic acid phenethyl ester on ischemia-reperfusion injury in comparison with alpha-tocopherol in rat kidneys. Urol Res 29:190–193

    Article  PubMed  CAS  Google Scholar 

  4. Giovannini L, Migliori M, Longoni BM, Das DK, Bertelli AA, Panichi V, Filippi C, Bertelli A (2001) Resveratrol, a polyphenol found in wine, reduces ischemia reperfusion injury in rat kidneys. J Cardiovasc Pharmacol 37:262–270

    Article  PubMed  CAS  Google Scholar 

  5. Akyol O, Herken H, Uz E, Fadillioglu E, Unal S, Sogut S, Ozyurt H, Savas HA (2002) The indices of endogenous oxidative and antioxidative processes in plasma from schizophrenic patients. The possible role of oxidant/antioxidant imbalance. Prog Neuropsychopharmacol Biol Psychiatry 26:995–1005

    Article  PubMed  CAS  Google Scholar 

  6. Serteser M, Koken T, Kahraman A, Yilmaz K, Akbulut G, Dilek ON (2002) Changes in hepatic TNF-alpha levels, antioxidant status, and oxidation products after renal ischemia/reperfusion injury in mice. J Surg Res. 107:234–240

    Article  PubMed  CAS  Google Scholar 

  7. Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn BW, Shaltiel S, Stantman ER (1990) Determination of carbonyl content in oxidatively modified proteins. In: Packer L, Glazer AN (eds) Methods in enzymology. Oxygen radicals in biological systems, vol 186. Academic, California, pp 464–478

  8. Conesa EL, Valero F, Nadal JC, Fenoy FJ, Lopez B, Arregui B, Salom MG (2001) N-acetyl-l-cysteine improves renal medullary hypoperfusion in acute renal failure. Am J Physiol Regul Integr Comp Physiol 281:R730–R737

    Google Scholar 

  9. Braga PC, Sasso MD, Zuccotti T (2000) Assessment of the antioxidant activity of the SH metabolite I of erdosteine on human neutrophil oxidative bursts. Drug Res 50:739–746

    CAS  Google Scholar 

  10. Yagmurca M, Fadillioglu E, Erdogan H, Ucar M, Sogut S, Irmak MK (2003) Erdosteine prevents doxorubicin-induced cardio-toxicity in rats. Pharmacol Res 48:377–382

    Article  PubMed  CAS  Google Scholar 

  11. Gurel A, Armutcu F, Cihan A, Numanoglu KV, Unalacak M (2004) Erdosteine improves oxidative damage in a rat model of renal ischemia-reperfusion injury. Eur Surg Res 36:206–209

    Article  PubMed  CAS  Google Scholar 

  12. Ozyurt H, Yildirim Z, Kotuk M, Yilmaz HR, Yagmurca M, Iraz M, Sogut S, Gergerlioglu S (2004) Cisplatin-iduced acute renal failure is ameliorated by erdosteine in a dose-dependent manner. J Appl Toxicol 24:269–275

    Article  PubMed  CAS  Google Scholar 

  13. Lowry O, Rosenbraugh N, Farr L. Rondall R (1951) Protein measurement with the folin-phenol reagent. J Biol Chem 183:265–275

    Google Scholar 

  14. Aebi H (1974) Catalase. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic, New York, pp 673–677

    Google Scholar 

  15. Esterbauer H, Cheeseman KH (1990) Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. In: Packer L, Glazer AN (eds) Methods in enzymology. Oxygen radicals in biological systems, vol 186. Academic, California, pp 407–421

  16. Sun Y, Oberley LW, Li Y (1988) A simple method for clinical assay of superoxide dismutase. Clin Chem 34:497–500

    Google Scholar 

  17. Prajda N, Weber G (1975) Malign transformation-linked imbalance: decreased XO activity in hepatomas. FEBS Lett 59:245–249

    Article  PubMed  CAS  Google Scholar 

  18. Rabb H, Ramirez G, Saba SR, Reynolds D, Xu J, Flavell R, Antonia S (1996) Renal ischemic-reperfusion injury in l-selectin deficient mice. Am J Physiol Renal Fluid Electrolyte Physiol 271:F408–F413

    CAS  Google Scholar 

  19. Anaya-Prado R, Toledo-Pereyra LH, Lentsch AB, Ward PA (2002) Ischemia/reperfusion injury. J Surg Res105:248–258

    Article  Google Scholar 

  20. Granger DN, Hollwarth ME, Parks DA (1986) Ischemia-reperfusion injury: role of oxygen-derived free radicals. Acta Physiol Scand 548(Suppl):47–63

    CAS  Google Scholar 

  21. Treska V, Kuntscher V, Hasman D, Neprasova P, Kobr J, Racek J, Trefil L, Hes O (2002) Importance of selenium for the influence of ischemia-reperfusion syndrome after kidney transplantation from a non-heart beating donor in a pig model. Transplant Proc 34:3057–3059

    Article  PubMed  CAS  Google Scholar 

  22. Joannidis M, Gstraunthaler G, Pfaller W (1990) Xanthine oxidase: evidence against a causative role in renal reperfusion injury. Am J Physiol 258:F232–F236

    PubMed  CAS  Google Scholar 

  23. Porter KA (1992) The kidney. In: Symmers WC (ed) In systemic pathology, vol 8. 3rd edn. Churchill-Livingstone, London, pp 491–498

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Authors and Affiliations

  1. Department of Physiology, Medical Faculty of Gaziosmanpasa University, Tip Fakultesi, 60100, Tokat, Turkey

    Hasan Erdogan

  2. Department of Physiology, Medical Faculty of Inonu University, Malatya, Turkey

    Ersin Fadillioglu

  3. Department of Histology and Embryology, Medical Faculty of Afyon Kocatepe University, Afyon, Turkey

    Murat Yagmurca

  4. Department of Histology and Embryology, Medical Faculty of Inonu University, Malatya, Turkey

    Muharrem Uçar

  5. Department of Histology and Embryology, Gulhane Military Medical Academy, Ankara, Turkey

    M. Kemal Irmak

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  1. Hasan Erdogan
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  2. Ersin Fadillioglu
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  3. Murat Yagmurca
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  4. Muharrem Uçar
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  5. M. Kemal Irmak
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Correspondence to Hasan Erdogan.

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Erdogan, H., Fadillioglu, E., Yagmurca, M. et al. Protein oxidation and lipid peroxidation after renal ischemia-reperfusion injury: protective effects of erdosteine and N-acetylcysteine. Urol Res 34, 41–46 (2006). https://doi.org/10.1007/s00240-005-0031-3

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  • DOI: https://doi.org/10.1007/s00240-005-0031-3

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