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Protein analysis of laser capture micro-dissected tissues revealed cell-type specific biological functions in developing barley grains

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Abstract

Both the nucellar projection (NP) and endosperm transfer cells (ETC) of the developing barley grain (harvested 8 days after flowering) were isolated by laser capture micro-dissection combined with pressure catapulting. Protein extracts were analyzed by nanoUPLC separation combined with ESI-Q-TOF mass spectrometry. The majority of the ∼160 proteins identified were involved in translation, protein synthesis, or protein destination. The NP proteome was enriched for stress defense molecules, while proteins involved in assimilate transport and the mobilization of nutrients were common to both the NP and the ETC. The combined qualitative and quantitative protein profiling allowed for the identification of several proteins showing tissue specificity in their expression, which underlines the distinct biological functions of these two tissues within the developing barley grain.

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Acknowledgments

Financial support from the German Federal Ministry of Education and Research (BMBF, 0313821A), the Federal State of Saxony-Anhalt, and the Cost Action FA0603, especially a grant to a short-term scientific mission (COST-STSM-FA0603-05188) is gratefully acknowledged. We thank Uta Siebert for her excellent technical assistance.

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Authors and Affiliations

  1. Applied Biochemistry Group, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466, Gatersleben, Germany

    Stephanie Kaspar, Hans-Peter Mock & Andrea Matros

  2. Seed Development, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466, Gatersleben, Germany

    Diana Weier & Winfriede Weschke

Authors
  1. Stephanie Kaspar
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  2. Diana Weier
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  3. Winfriede Weschke
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  4. Hans-Peter Mock
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  5. Andrea Matros
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Corresponding author

Correspondence to Andrea Matros.

Additional information

Published in the special issue Mass Spectrometry (DGMS 2010) with guest editors Andrea Sinz and Jürgen Schmidt.

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Kaspar, S., Weier, D., Weschke, W. et al. Protein analysis of laser capture micro-dissected tissues revealed cell-type specific biological functions in developing barley grains. Anal Bioanal Chem 398, 2883–2893 (2010). https://doi.org/10.1007/s00216-010-4120-y

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  • DOI: https://doi.org/10.1007/s00216-010-4120-y

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