Abstract
We exploited the newly developed amplified fragment length polymorphism (AFLP) technique to study the polymorphism, distribution and inheritance of AFLP markers with a doubled haploid rice population derived from ‘IR64’/‘Azucena’. Using only 20 pairs of primer combinations, we detected 945 AFLP bands of which 208 were polymorphic. All 208 AFLP markers were mapped and distributed over all 12 chromosomes. When these were compared with RFLP markers already mapped in the population, we found the AFLP markers to be highly polymorphic in rice and to follow Mendelian segregation. As linkage map of rice can be generated rapidly with AFLP markers they will be very useful for marker-assisted backcrossing.
Similar content being viewed by others
References
Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208–218
Becker J, Vos P, Kuiper M, Salamini F, Heun M (1995) Combined mapping of AFLP and RFLP markers in barley. Mol Gen Genet 249:65–73
Causse M, Fulton TM, Cho YG, Ahn SN, Chunwongse EJ, Wu K, Xiao J, Yu Z, Ronald PC, Harrington SB, Second G, McCouch SR, Tanksley SD (1994) Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics 138:1251–1274.
Dellaporta SL, Wood J, Hick JB (1983) A plant DNA mini preparation; version II. Plant Mol Biol Rep 1:19–21
Guiderdoni E, Galinato E, Luistro J, Vergara G (1992) Anther culture of tropical japonica X indica hybrids of rice (Oryza sativa L.). Euphytica 62:219–224
Hodges TK, Peng J, Lyznik LA, Koetje DS (1991) Transformation and regeneration of rice protoplasts. In: Khush G, Toenniessen G (eds) Rice biotechnology. IRRI, Manila, Philippines, pp 157–174
Huang N, McCouch SR, Mew T, Parco A, Guiderdoni E (1994) Development of a RFLP map from a doubled haploid population of rice. Rice Genet Newsl 11:134–137
Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175
Kurata N, Nagamura Y, Yamamoto K, Harushima Y, Sue N, Wu J, Antonio BA, Shomura A, Shimizu T, Lin S-Y, Inoue T, Fukuda A, Shimano T, Kuboki Y, Toyama T, Miyamoto Y, Kirihara T, Hayasaka K, Miyao A, Monna L, Zhong HS, Tamura Y, Wang Z-X, Momma T, Umehara Y, Yano M, Sasaki T, Minobe Y (1994) A-300-kilobase interval genetic map of rice including 883 expressed sequences. Nat Genet 8:365–372
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Litt M, Luty J (1989) A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle action gene. Am J Hum Genet 44:391–401
Lorieux M, Petrov M, Huang N, Faure J, Guiderdoni E, Chesquiere A (1996) Aroma in rice is controlled by a major gene and at least two minor QTLs. Theor Appl Genet (in press)
Moore G, Devos KM, Wang Z, Gale MD (1995) Grasses line up and form a circle. Curr Biol 5:737–739
Olson M, Hood L, Cantor C, Botstein D (1989) A common language for physical mapping of the human genome. Science 245: 1434–1435
Rafalski JA, Tingey SV (1993) Genetic diagnostics in plant breeding:RAPDS, microsatellites and machines. Trends Genet 9: 275–280
Tanksley SD, Young ND, Patterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: new tools for an old science. Bio/Technology 7:257–264
Thomas CM, Vos P, Zabeau M, Jones DA, Norcott KA, Chadwick BP, Jones JDG (1995) Identification of amplified restriction fragment polymorphism (AFLP) markers tightly linked to the tomato Cf-9 gene for resistance to Cladosporium fulvum. Plant J 8:785–794
Vos P, Hogers R, Bleeker M, Reijans M, Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP:a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey VS (1990) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535
Zabeau M, Vos P 1993 Selective restriction fragment amplification : a general method for DNA fingerprinting. European Patent Application number : 92402629.7, Publication number 0534858 Al
Author information
Authors and Affiliations
Additional information
Communicated by G. Wenzel
Rights and permissions
About this article
Cite this article
Maheswaran, M., Subudhi, P.K., Nandi, S. et al. Polymorphism, distribution, and segregation of AFLP markers in a doubled haploid rice population. Theoret. Appl. Genetics 94, 39–45 (1997). https://doi.org/10.1007/s001220050379
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s001220050379