Abstract
The ovary plays a central role in oogenesis and gonadal hormone secretion. Proteomic analysis is a valuable approach for gaining an increased understanding of the molecular nature of the ovary. In this work, two-dimensional electrophoresis for protein separation followed by matrix-assisted laser desorption/ionization mass spectrometry and database searches, identified 231 protein spots corresponding to 138 individual proteins that were found in gels representing both the follicular and luteal phases. The data were used to construct a database online (http://reprod.njmu.edu.cn/2d). The identified proteins were functionally classified into seven groups: (1) cell signaling/communication, (2) cell division, (3) gene/protein expression, (4) metabolism, (5) cell structure and motility, (6) cell/organism defense, and (7) unclassified. Among the proteins identified, 47% had not been previously reported in the human ovary. In addition, a number of disease-related proteins were identified in this protein map, including some cancer- and polycystic ovarian syndrome-related proteins. Two proteins with phosphorylation were verified by Western blot analysis. Comparison of protein abundance between follicular and luteal stages produced seven protein spots that had been identified in our database. This study provides a preliminary reference map of normal human ovary that will form a basis for comparative studies on normal and pathological conditions of the human ovary and may serve as a potential tool for clinical diagnosis, therapeutics, and prognosis.
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Abbreviations
- PCOS:
-
polycystic ovarian syndrome
- 2-D PAGE:
-
two-dimensional polyacrylamide gel electrophoresis
- CHAPS:
-
3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate
- IPG:
-
immobilized pH gradient
- SDS:
-
sodium dodecyl sulphate
- APS:
-
ammonium persulfate
- TEMED:
-
N,N,N′,N′-tetramethylethylenediamine
- ACN:
-
acetonitrile
- TFA:
-
trifluoroacetic acid
- DTT:
-
dithiothreitol
- hnRNP A2/B1:
-
heterogeneous nuclear ribonucleoproteins A2/B1
- IEF:
-
Isoelectric focusing
- MALDI-TOF:
-
matrix-assisted laser desorption/ionization time-of-flight
- pI:
-
isoelectric points
- MWs:
-
molecular weights
- ANX:
-
annexin
- HCC:
-
hepatocellular carcinoma
- VDAC:
-
voltage-dependent anion-selective channel
- BAK:
-
BCL2 homologous antagonist/killer
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This work was supported by the National Natural Science Foundation of China (30170356).
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L. Wang and Y.-F. Zhu contributed equally to this work
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Wang, L., Zhu, YF., Guo, XJ. et al. A two-dimensional electrophoresis reference map of human ovary. J Mol Med 83, 812–821 (2005). https://doi.org/10.1007/s00109-005-0676-y
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DOI: https://doi.org/10.1007/s00109-005-0676-y