Abstract
Neem hull waste (containing a high amount of lignin and other phenolic compounds) was used for lignin peroxidase production byPhanerochaete chrysosporum under solid-state fermentation conditions. Maximum decolorization achieved by partially purified lignin peroxidase was 80% for Porocion Brilliant Blue HGR, 83 for Ranocid Fast Blue, 70 for Acid Red 119 and 61 for Navidol Fast Black MSRL. The effects of different concentrations of veratryl alcohol, hydrogen peroxide, enzyme and dye on the efficiency of decolorization have been investigated. Maximum decolorization efficiency was observed at 0.2 and 0.4 mmol/L hydrogen peroxide, 2.5 mmol/L veratryl alcohol and pH 5.0 after a 1-h reaction, using 50 ppm of dyes and 9.96 mkat/L of enzyme.
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Buckley K.F., Dobson A.D.W.: Extracellular ligninolytic enzyme production and polymeric dye decolorization in immobilized cultures ofChrysosporium lignorum CL 1.Biotechnol. Lett.20, 301–306 (1998).
Darah I., Ibrahim C.O.: Laboratory-scale production of lignin-degrading enzymes by free and entrapped cells ofPhanerochaete chrysosporium in a tubular air-lift bioreactor.Folia Microbiol.43, 161–168 (1998).
Das N., Chakraborty T.K., Mukherjee M.: Purification and characterization of laccase 1 fromPleurotus florida.Folia Microbiol.45, 447–451 (2000).
Gabriel J., Shah V., Nesměrák K., Baldrian P., Nerud F.: Degradation of polycyclic aromatic hydrocarbons by the copper(II) hydrogen peroxide system.Folia Microbiol.45, 573–576 (2000).
Hattaka A.: Lignin modifying enzymes from selected white rot fungi: production and role in lignin degradation.FEMS Microbiol. Rev.13, 125–135 (1994).
Kahraman S., Yešilada O. Effect of spent cotton stalks on color removal and chemical oxygen demand lowering in olive oil mill wastewater by white rot fungi.Folia Microbiol.44, 673–676 (1999).
Kahraman S., Yešilada O.: Industrial and agricultural wastes as substrates for laccase production by white-rot fungi.Folia Microbiol.45, 133–136 (2000).
Katić M., Frantar J., Grgić I., Podgornik H., Perdih A.: Polyoxyethylene stimulates lignin peroxidase production inPhanerochaete chrysosporium.Folia Microbiol.43, 631–634 (1998).
Kirk T.K., Farrel R.L.: Enzymatic combustion: the microbial degradation of lignin.Ann. Rev. Microbiol.41, 464–505 (1987).
Knapp J.S., Newby P.S., Reece L.P.: Decolorization of dyes by wood-rotting fungi.Enzyme Microb. Technol.17, 664–668 (1994).
Kotterman M.J.J., Rietberg H.J., Hage A., Field J.A.: Polycyclic aromatic hydrocarbon oxidation by the white-rot fungusBjerkandera sp. strain BOS55 in the presence of nonionic surfactants.Biotechnol. Bioeng.57, 220–227 (1997).
Krčmar P., Ulrich R.: Degradation of polychlorinated biphenyl mixtures by the lignin-degrading fungusPhanerochaete chrysosporium.Folia Microbiol.43, 79–84 (1998).
Martens R., Zadrazil F.: Screening of white-rot fungi for their ability to mineralize polycyclic aromatic hydrocarbons in soil.Folia Microbiol.43, 97–103 (1998).
Mehna A., Bajpai P., Bajpai P.K.: Studies on decolorization of effluent from a small pulp mill utilizing agriresidues withTrametes versicolor.Enzyme Microb. Technol.17, 18–22 (1993).
Sam M., Yeşilada O.: Decolorization of Orange II dye by white-rot fungi.Folia Microbiol.46, 143–146 (2001).
Sani R.K., Azmi W., Banerjee U.C.: Comparison of static and shake culture in the decolorization of textile dyes and dye effluents byPhanerochaete chrysosporium.Folia Microbiol.43, 85–88 (1998).
Shah V., Garg N., Madamwar D.: Exopolysaccharides production by a marine cyanobacteriumCyanothece sp. and its application in dye removal by its gelation phenomenon.Appl. Biochem. Biotechnol.82, 81–90 (1999).
Tien M., Krik T.K.: Lignin peroxidase ofPhanerochaete chrysosporium.Meth. Enzymol.161, 238–243 (1988).
Ünyayar S., Ünal E., Ünyayar A.: Relationship between production of 3-indoleacetic acid and peroxidase-laccase activities depending on the culture periods inFunalia trogii (Trametes trogii).Folia Microbiol.46, 123–126 (2001).
Young L., Yu J.: Ligninase-catalyzed decolorization of synthetic dyes.Water Sci. Technol.31, 1187–1197 (1997).
Zheng Z., Levin R.E., Pinkam J.L., Shetty K.: Decolorization of polymeric dyes by aPenicillium isolate.Proc. Biochem.34, 31–37 (1999).
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Verma, P., Madamwar, D. Decolorization of synthetic textile dyes by lignin peroxidase ofPhanerochaete chrysosporium . Folia Microbiol 47, 283–286 (2002). https://doi.org/10.1007/BF02817653
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DOI: https://doi.org/10.1007/BF02817653