Abstract
ACurvularia sp. isolated from soil was found to contain laccase activity toward guaiacol as substrate. The organism produced an extracellular laccase in a medium containing yeast extract, peptone and dextrose. Initial medium pH 4.0 and cultivation temperature 30°C were found to be most suitable for maximum enzyme production. The optimum pH and temperature for laccase activity were found to be 5.2 and 50°C, respectively. Under optimum conditions, the enzyme had aK m (guaiacol) of 0.75 mmol/L and aV of 1.50 CU min−1 ml−1. Some divalent metal ions inhibited laccase activity at very low concentrations.
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Banerjee, U.C., Vohra, R.M. Production of laccase byCurvularia sp.. Folia Microbiol 36, 343–346 (1991). https://doi.org/10.1007/BF02814506
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DOI: https://doi.org/10.1007/BF02814506