Skip to main content
SpringerLink
Log in

Extracellular Laccase from a Newly Isolated Psychrotolerant Strain of Cladosporium tenuissimum (NFCCI 2608)

  • Research Article
  • Published:
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

A newly isolated psychrotolerant fungus from cold desert in Indian Himalaya has been investigated for laccase production. Based on phenotypic and genotypic characters, the fungus has been identified as Cladosporium tenuissimum. The fungus could grow between 4 and 30 °C (optimum 24 °C). In ABTS plate assays, the efficiency for enzyme production was relatively higher at low temperature. In quantitative estimations, the laccase activity was recorded almost twice (15.10 ± 0.78 UL−1) at 14 °C in comparison to the activity (8.05 ± 0.94 UL−1) at 24 °C. Optimum pH for fungal growth as well as laccase production was 5.5. The molecular mass of laccase determined by native PAGE was approximately 48 kDa. Km and Vmax values of the enzyme were determined as 0.21 mM and 0.38 mM min−1, respectively. Addition of carbon and nitrogen sources and organic solvents (supplements) affected enzyme activity significantly; ethanol being the best enhancer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Desai SS, Nityanand C (2011) Microbial laccases and their applications: a review. Asian J Biotechnol 3(2):98–124

    Article  CAS  Google Scholar 

  2. Riva S (2006) Laccases: blue enzymes for green chemistry. Trends Biotechnol 24(5):219–226

    Article  CAS  PubMed  Google Scholar 

  3. Kunamneni A, Ballesteros A, Plou FJ, Alcalde M (2007) Fungal laccase: a versatile enzyme for biotechnological applications. In: Mendez-Vilas A (ed) Communicating current research and educational topics and trends in applied microbiology. Formex, Badajoz, pp 233–245

    Google Scholar 

  4. Madhavi V, Lele SS (2009) Laccase: properties and Applications. BioResources 4(4):1694–1717

    Google Scholar 

  5. Ghosh A (2012) Exploitation of microbial diversity for novel products. Proc Natl Acad Sci India 82(2):301–304

    Google Scholar 

  6. Pandey A, Palni LMS (2007) The rhizosphere effect in trees of the Indian central Himalaya with special reference to altitude. Appl Ecol Environ Res 5(1):93–102

    Article  Google Scholar 

  7. Venkataraman K (2012) Biodiversity and its conservation. Proc Natl Acad Sci India 82(2):271–282

    Google Scholar 

  8. Dhakar K, Sharma A, Pandey A (2014) Cold, pH and salt tolerant Penicillium spp. inhabit the high altitude soils in Himalaya, India. World J Microbiol Biotechnol 30:1315–1324

    Article  CAS  PubMed  Google Scholar 

  9. Ghildiyal A, Pandey A (2008) Isolation of cold tolerant antifungal strains of Trichoderma sp. from glacial sites of Indian Himalayan region. Res J Microbiol 3(8):559–564

    Article  Google Scholar 

  10. Gunde-Cimerman N, Sonjak S, Zalar P, Frisvad JC, Diderichsen B, Plemenitas A (2003) Extremophilic fungi in arctic ice: a relationship between adaptation to low temperature and water activity. Phys Chem Earth 28:1273–1278

    Article  Google Scholar 

  11. Rinu K, Pandey A (2010) Temperature-dependent phosphate solubilization by cold- and pH-tolerant species of Aspergillus isolated from Himalayan soil. Mycoscience 51(4):263–271

    Article  CAS  Google Scholar 

  12. Rinu K, Pandey A, Palni LMS (2012) Utilization of psychrotolerant phosphate solubilizing fungi under low temperature conditions of the mountain ecosystem. In: Satyanarayana T, Johri BN, Prakash A (eds) Microorganisms in sustainable agriculture and biotechnology. Springer, Dordrecht, pp 77–90 part1

    Chapter  Google Scholar 

  13. Sahay S, Lone MA, Jain P, Singh P, Chouhan D, Shezad F (2013) Cold-active moulds from Jammu and Kashmir, India as potential source of cold-active enzymes. Am J Curr Microbiol 1:1–13

    Google Scholar 

  14. Dhakar K, Pandey A (2013) Laccase production from a temperature and pH tolerant fungal strain of Trametes hirsuta (MTCC11397). Enzyme Res (Article ID 869062)

  15. Rinu K, Pandey A (2011) Slow and steady phosphate solubilization by a psychrotolerant strain of Paecilomyces hepiali (MTCC 9621). World J Microbiol Biotechnol 27(5):1055–1062

    Article  Google Scholar 

  16. White TJ, Bruns TD, Lee SB, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322

    Google Scholar 

  17. Kumar S, Nei M, Dudley J, Tamura K (2008) MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9(4):299–306

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Kirk TK, Farrell RL (1987) Enzymatic combustion- the microbial-degradation of lignin. Annu Rev Microbiol 41:465–505

    Article  CAS  Google Scholar 

  19. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  CAS  PubMed  Google Scholar 

  20. Han MJ, Choi HT, Song HG (2005) Purification and characterization of laccase from the white rot fungus Trametes versicolor. J Microbiol 43(6):555–560

    CAS  PubMed  Google Scholar 

  21. Bensch K, Braun U, Groenewald JZ, Crous PW (2012) The genus Cladosporium. Stud Mycol 72:1–401

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Nasini G, Arnone A, Assante G, Bava A, Moricca S, Ragazzi A (2004) Secondary mould metabolites of Cladosporium tenuissimum, a hyperparasite of rust fungi. Phytochem 65:2107–2111

    Article  CAS  Google Scholar 

  23. Aslam MS, Aishy A, Samra ZQ, Gull I, Athar MA (2012) Identification, purification and characterization of a novel extracellular laccase from Cladosporium cladosporioides. Biotechnol Biotechnol Equip. doi:10.5504 BBEQ.2012.0107

  24. Vijaykumar M, Halaburgi VM, Sharma S, Sinha M, Singh TP, Karegoudar TB (2011) Purification and characterization of a thermostable laccase from the ascomycetes Cladosporium cladosporioides and its applications. Process Biochem 46:1146–1152

    Article  Google Scholar 

  25. Zadrazil F, Gonser A, Lang E (1999) Influence of incubation temperature on the secretion of extracellular ligninolytic enzymes of Pleurotus sp. and Dichomitus squalens into soil. In: Proceedings of the Conference on Enzymes in the Environment. Activity, Ecology and Applicants, Granada, 12–16 July 1999

  26. Elsayed MA, Hassan MM, Elshafei AM, Haroun BM, Othman AM (2012) Optimization of cultural and nutritional parameters for the production of laccase by Pleurotus ostreatus ARC280. Br Biotechnol J 2(3):115–132

    Article  CAS  Google Scholar 

  27. Dhakar K, Jain R, Tamta S, Pandey A (2014) Prolonged laccase production by a cold and pH tolerant strain of Penicillium pinophilum (MCC 1049) isolated from a low temperature environment. Enzyme Res (Article ID 120708)

  28. Dekker RFH, Barbosa AM, Giese EC, Godoy SD, Covizzi LG (2007) Influence of nutrients on enhancing laccase production by Botryosphaeria rhodina MAMB-05. Int Microbiol 10(3):177–185

    CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The Director, GB Pant Institute of Himalayan Environment and Development, Almora, is gratefully acknowledged for extending the facilities. Agharkar Research Institute, Pune is thanked for nucleotide sequencing of the fungus. Ministry of Environment, Forests and Climate Change, Govt. of India is thanked for financial support. Senior author is thankful to Indian Council of Medical Research (ICMR), Govt. of India, New Delhi, for awarding the research fellowship.

Author information

Authors and Affiliations

  1. Biotechnological Applications, G. B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora, 263 643, Uttarakhand, India

    Kusum Dhakar & Anita Pandey

Authors
  1. Kusum Dhakar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anita Pandey
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Anita Pandey.

Additional information

Extracellular laccase from a newly isolated psychrotolerant strain of Cladosporium tenuissimum (NFCCI 2608).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Dhakar, K., Pandey, A. Extracellular Laccase from a Newly Isolated Psychrotolerant Strain of Cladosporium tenuissimum (NFCCI 2608). Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 86, 685–690 (2016). https://doi.org/10.1007/s40011-015-0507-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40011-015-0507-z

Keywords

Search

Navigation