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Establishment of a cell line fromHeliothis Armigera (Hbn.) (Lepidoptera: Noctuidae)

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Summary

A new cell line, designated BCIRL-HA-AM1, derived from ovaries of female pupas ofHeliothis armigera (Hbn.) has been established. Both oval and round cells were found in BCIRL-HA-AM1. Chromosomes were typical of lepidoptera (i.e. short condensed rods and spheres; a majority of cells were polyploid). TheH. armigera line was distinguished fromH. zea andH. virescens cell lines as well as two other noctuid cell lines,Trichoplusia ni (Hübner) andSpodoptera frugiperda (J. E. Smith), by use of isoelectric focusing and the enzyme phosphoglucoisomerase. This cell line would not replicate (i.e. produce polyhedral inclusion bodies or show an increase in virus titer) the single-embedded nuclear polyhedrosis virus (NPV) ofH. zea nor the multiple-embedded NPV ofH. armigera orAutographa californica Speyer.

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References

  1. McIntosh, A. H.; Andrews P. A.; Ignoffo, C. M. Establishment of two continuous cell lines ofHeliothis virescens (F.) (Lepidoptera: Noctuidae). In Vitro 17: 649–650; 1981.

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Author notes

  1. Chen Quhou (visiting scientist)

    Present address: Central China Teachers College, Wuhan, Hupeh, China

Authors and Affiliations

  1. Agricultural Research Service, Biological Control of Insects Research Laboratory, U.S. Department of Agriculture, 65205, Columbia, Missouri

    Arthur H. McIntosh, Carlo M. Ignoffo, Chen Quhou (visiting scientist) & Mike Pappas

Authors
  1. Arthur H. McIntosh
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  2. Carlo M. Ignoffo
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  3. Chen Quhou
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  4. Mike Pappas
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Additional information

This paper reflects the results of research only; mention of a proprietary product does not constitute a recommendation by the U.S. Department of Agriculture.

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McIntosh, A.H., Ignoffo, C.M., Quhou, C. et al. Establishment of a cell line fromHeliothis Armigera (Hbn.) (Lepidoptera: Noctuidae). In Vitro 19, 589–590 (1983). https://doi.org/10.1007/BF02619571

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  • DOI: https://doi.org/10.1007/BF02619571

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