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Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants

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Abstract

Progress in plant molecular biology has depended heavily on the availability of effective vectors for plant cell transformation and heterologous expression. In this paper we describe the structures of a wide array of plasmids which have proved extremely effective in (a) plant transformation, (b) expression of heterologous genes and (c) assaying the activity of transposons in transgenic plants. Constructs that confer resistance to kanamycin, hygromycin, streptomycin, spectinomycin and phosphinotricin, or that confer β-glucuronidase (GUS) gene expression are presented. Binary vector constructs that carry polylinkers of the pUC and Bluescript types are also described. Plasmids that permit the expression of any heterologous reading frame from either nopaline synthase (nos) or octopine synthase (ocs) promoters, as well as the cauliflower mosaic virus 35S promoter, using either the nopaline synthase or octopine synthase 3′ polyadenylation sequences, are presented. These constructs permit a choice of orientation of the resulting transgene of interest, relative to the orientation of the selection marker gene. Most of the plasmids described here are publicly available.

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Author notes

  1. L. Shlumukov

    Present address: Department of Botany, University of Nottingham, University Park, NG7 2RD, Nottingham, UK

Authors and Affiliations

  1. The Sainsbury Laboratory, John Innes Centre for Plant Science Research, Norwich Research Park, Colney, NR 4 7UK, Norwich, UK

    Jonathan D. G. Jones, L. Shlumukov, J. English, S. R. Scofield, G. J. Bishop & K. Harrison

  2. Department of Plant Pathology, University of California, Berkeley, 94705, Berkeley, CA, USA

    F. Carland

  3. Institute of Cell Biology and Genetic Engineering, Ukrainian Academy of Sciences, Kiev, Ukraine

    L. Shlumukov

  4. Advanced Genetic Sciences (now DNA Plant Technology Corporation), 6701 San Pablo Avenue, 94608, Oakland, CA, USA

    Jonathan D. G. Jones, L. Shlumukov & F. Carland

Authors
  1. Jonathan D. G. Jones
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Jones, J.D.G., Shlumukov, L., Carland, F. et al. Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants. Transgenic Research 1, 285–297 (1992). https://doi.org/10.1007/BF02525170

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  • DOI: https://doi.org/10.1007/BF02525170

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