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Functional characterization of rat chemokine macrophage inflammatory protein-2

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Abstract

Expression of mRNA for the C-X-C chemokine, macrophage inflammatory protein-2 (MIP-2), is induced during acute inflammation in rat models of disease. We have characterized the phlogistic potential of rat recombinant MIP-2 (rMIP-2) protein in vitro and in vivo. Recombinant MIP-2 caused marked PMN chemotaxis in vitro, with peak chemotactic activity at 10 nM. Incubation of whole blood with rMIP-2 caused a significant loss of L-selectin and a significant increase in Mac-1 expression on the PMN surface. Under similar conditions rMIP-2 also caused a modest respiratory burst in PMNs. The intratracheal instillation of 10 and 50μg of rMIP-2 caused a significant influx of PMNs into the airspace of the lungs. Rat MIP-2 is a potent neutrophil chemotactic factor capable of causing neutrophil activation and is likely to function in PMN recruitment during acute inflammation in rat disease models.

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Authors and Affiliations

  1. Physiology Program, Harvard School of Public Health, USA

    Charles W. Frevert, Anthony Farone, Hadi Danaee, Joseph D. Paulauskis & Lester Kobzik

  2. Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts

    Lester Kobzik

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  1. Charles W. Frevert
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  2. Anthony Farone
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  3. Hadi Danaee
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  4. Joseph D. Paulauskis
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  5. Lester Kobzik
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Frevert, C.W., Farone, A., Danaee, H. et al. Functional characterization of rat chemokine macrophage inflammatory protein-2. Inflammation 19, 133–142 (1995). https://doi.org/10.1007/BF01534386

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