Abstract
By sodium dodecyl sulfate-polyacrylamide gel electrophoresis, we analyzed polypeptides solubilized from spore walls of the fission yeast Schizosaccharomyces pombe. A prominent protein band whose apparent M r was 23,000 was found on stained gels. This major spore wall polypeptide (23K) existed most abundantly in the cellular homogenate of sporulating cultures, while the corresponding band was almost absent in the extract from nonsporulating cultures.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- EDTA:
-
ethylenediamine tetraacetate
- ME:
-
2-mercaptoethanol
- PMSF:
-
phenylmethanesulfonyl fluoride
- SDS:
-
sodium dodecyl sulfate
- Tris:
-
tris (hydroxymethyl)-aminomethane
References
Briley MS, Illingworth RF, Rose AH, Fisher DJ (1970) Evidence for a surface protein layer on the Saccharomyces cerevisiae ascospore. J Bacteriol 104:588–589
Egel R (1971) Physiological aspects of conjugation in fission yeast. Planta 98:89–96
Egel R, Egel-Mitani M (1974) Premeiotic DNA synthesis in fission yeast. Exp Cell Res 88:127–134
Friedmann KL, Egel R (1978) Protein patterns during sporulation in fission yeast. Z Naturforsch 33:84–91
Kraig E, Haber JE (1980) Messenger ribonucleic acid and protein metabolism during sporulation of Saccharomyces cerevisiae. J Bacteriol 144:1098–1112
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680–685
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Nishi K, Shimoda C, Hayashibe M (1978) Germination and outgrowth of Schizosaccharomyces pombe ascospores isolated by Urografin density gradient centrifugation. Can J Microbiol 24:893–897
Sando N, Oguchi T, Nagano M, Osumi M (1980) Morphological changes in ascospores of Saccharomyces cerevisiae during aerobic and anaerobic germination. J Gen Appl Microbiol 26:403–412
Shimoda C (1980) Differential effect of glucose and fructose on spore germination in the fission yeast, Schizosaccharomyces pombe. Can J Microbiol 26:741–745
Snider IJ, Miller JJ (1966) A serological comparison of vegetative cell and ascus walls and the spore coat of Saccharomyces cerevisiae. Can J Microbiol 12:485–488
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shimoda, C. Existence of a major spore wall protein (23K) in the fission yeast Schizosaccharomyces pombe: A possible sporulation-specific protein. Arch. Microbiol. 136, 26–27 (1983). https://doi.org/10.1007/BF00415605
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00415605