Summary
Twenty six riboflavine deficient mutants of Saccharomyces cerevisiae were isolated. They can be divided by biochemical methods into four classes, accumulating (i) no specific product (S), (ii) 2,5-diamino-6-hydroxy-4-ribitylaminopyrimidine (AP), (iii) 5-amino-2,6-dihydroxy-4-ribitylaminopyrimidine (HP), (iv) 6,7-dimethyl-8-ribityllumazine (LU).
In genetic experiments six complementation groups were found. Complementation group I is congruent with class S, group II with AP, III and IV with HP and V and VI with LU. In the crosses tested so far, no linkage between complementation groups could be detected by tetrad analysis. Intragenic complementation was found within complementation group V (mutants accumulating LU).
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Communicated by F. Kaudewitz
Genetic experiments were performed at the “Forstbotanisches Institut der Universität Freiburg”. The senior author (O.O.) wishes to thank Prof. Dr. Dr. Hans Marquardt for generously providing the facilities of the Forstbotanisches Institut. This work was supported by a grant of the “Deutsche Forschungsgemeinschaft” to O.O.
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Oltmanns, O., Bacher, A., Lingens, F. et al. Biochemical and genetic classification of riboflavine deficient mutants of Saccharomyces cerevisiae . Molec. Gen. Genet. 105, 306–313 (1969). https://doi.org/10.1007/BF00277585
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DOI: https://doi.org/10.1007/BF00277585