Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 950)
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Table of contents (26 protocols)
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Front Matter
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Light Microscopy
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Front Matter
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Light Microscopy
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Electron Microscopy
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Front Matter
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Keywords
About this book
For more than a century, microscopy has been a centerpiece of extraordinary discoveries in biology. Along the way, remarkable imaging tools have been developed allowing scientists to dissect the complexity of cellular processes at the nano length molecular scales. Nanoimaging: Methods and Protocols presents a diverse collection of microscopy techniques and methodologies that provides guidance to successfully image cellular molecular complexes at nanometer spatial resolution. The book's four parts cover: (1) light microscopy techniques with a special emphasis on methods that go beyond the classic diffraction-limited imaging; (2) electron microscopy techniques for high-resolution imaging of molecules, cells and tissues, in both two and three dimensions; (3) scanning probe microscopy techniques for imaging and probing macromolecular complexes and membrane surface topography; and (4) complementary techniques on correlative microscopy, soft x-ray tomography and secondary ion mass spectrometry imaging. Written in the successful format of the Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Nanoimaging: Methods and Protocols highlights many of the most exciting possibilities in microscopy for the investigation of biological structures at the nano length molecular scales.
Reviews
From the reviews:
“The book is divided into 4 parts, each containing several chapters pertaining to a given field. … chapters are largely composed of detailed protocols describing a specific experimental procedure. Figures are mostly clear, and the tables and index are helpful. … I highly recommend this book to academic radiologists, imaging scientists, and molecular biologists.” (E. Edmund Kim, The Journal of Nuclear Medicine, Vol. 54 (5), May, 2013)
“The present volume … contains 28 protocols in diverse types of microscopy enabling scientists to obtain images of isolated molecular complexes, cells and tissues at nanoscale spatial resolution … . Each chapter has a standard structure … a clear style and useful illustrations. … this book should be read not only by very specialized scientists, but also by those who usually work with (very) classic microscopic methods, to open their minds and increase their appetite for more precise and complex measurements done on complex structures.” (Ioan I. Ardelean, Romanian Biochemical Journals, Vol. 50 (2), 2013)
Editors and Affiliations
Bibliographic Information
Book Title: Nanoimaging
Book Subtitle: Methods and Protocols
Editors: Alioscka A. Sousa, Michael J. Kruhlak
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-137-0
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-62703-136-3Published: 21 October 2012
Softcover ISBN: 978-1-4939-5961-7Published: 23 August 2016
eBook ISBN: 978-1-62703-137-0Published: 20 October 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 510
Number of Illustrations: 56 b/w illustrations, 76 illustrations in colour
Topics: Imaging / Radiology, Biological Microscopy