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4Pi Microscopy

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Book cover Nanoimaging

Part of the book series: Methods in Molecular Biology ((MIMB,volume 950))

Abstract

Optical microscopy has become a key technology in the life sciences today. Its noninvasive nature provides access to the interior of intact and even living cells, where specific molecules can be precisely localized by fluorescent tagging. However, the attainable 3D resolution of an optical microscope has long been hampered by a comparatively poor resolution along the optic axis. By coherent focusing through two objective lenses, 4Pi microscopy improves the axial resolution by three- to fivefold. This primer is intended as a starting point for the design and operation of a 4Pi microscope of type A.

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Author information

Authors and Affiliations

  1. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany

    Roman Schmidt

  2. Department of Optical Nanoscopy, German Cancer Research Center, Heidelberg, Germany

    Johann Engelhardt & Marion Lang

Authors
  1. Roman Schmidt
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  2. Johann Engelhardt
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  3. Marion Lang
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Corresponding author

Correspondence to Roman Schmidt .

Editor information

Editors and Affiliations

  1. Nat. Inst. Biomedical Imaging & Bioengin, National Institutes of Health, South Drive 13, Bethesda, 20892, Maryland, USA

    Alioscka A. Sousa

  2. , National Cancer Institute, National Institutes of Health, Center Drive 10, Bethesda, 20892, USA

    Michael J. Kruhlak

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© 2013 Springer Science+Business Media, LLC

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Schmidt, R., Engelhardt, J., Lang, M. (2013). 4Pi Microscopy. In: Sousa, A., Kruhlak, M. (eds) Nanoimaging. Methods in Molecular Biology, vol 950. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-137-0_3

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  • DOI: https://doi.org/10.1007/978-1-62703-137-0_3

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-136-3

  • Online ISBN: 978-1-62703-137-0

  • eBook Packages: Springer Protocols

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