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Bovine Retinal Microvascular Pericytes

Isolation, Propagation, and Identification

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Angiogenesis Protocols

Part of the book series: Methods in Molecular Medicineā„¢ ((MIMM,volume 46))

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Abstract

The growth of new capillaries from existing vessels (angiogenesis) is of fundamental importance in wound healing and in pathological situations such as proliferative diabetic retinopathy (1), rheumatoid arthritis (2), and tumor growth. Consequently, considerable interest in vascular cell biology has arisen in apparently disparate clinical and experimental fields. Held in common, however, is the hope that an understanding of the cellular and molecular mechanisms that regulate angiogenesis will lead to novel therapeutic agents and targets.

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References

  1. Merimee, T. J. (1990) Diabetic Retinopathy: A Synthesis of Perspectives. N. Engl. J. Med. 322, 978ā€“983.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  2. Folkman, J. (1995) Angiogenesis in cancer, vascular, rheumatoid and other disease. Nature Med. 1, 27ā€“31.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  3. Saunders, K. B., Antonelli-Orlidge, A., Smith, S., and Dā€²Amore, P. A. (1990) Cell communication and the control of endothelial cell growth: In: Endothelial Cell Function in Diabetic Microangiopathy: Problems in Methodology and Clinical Aspects. Frontiers in Diabetes, (Molinatti, G. M., Bar, R. S., Belfiore, F., and Porta, M., eds.) Basel Karger 9, 183ā€“191.

    Google ScholarĀ 

  4. Meezan, E., Klaus, B., and Carlson, E. C. (1974) Isolation of a purified preparation of metabolically active retinal blood vessels. Nature 251, 65ā€“67.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  5. Klaus, B., Meezan, E., and Carlson, E. C. (1974) Isolated brain microvessels: A purified, metabolically active preparation from bovine cerebral cortex. Science 185, 953ā€“955.

    ArticleĀ  Google ScholarĀ 

  6. Buzney, S. M., Frank, R. N., and Robison, W. G., Jr. (1975) Retinal capillaries:Proliferation of mural cells in vitro. Science 190, 985ā€“986.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  7. Del Vecchio, P., Ryan, U. S., and Ryan, J. W. (1977) Isolation of capillary segments from rat adrenal gland. J. Cell Biol. 75, 73a.

    Google ScholarĀ 

  8. Folkman, J., Haudenschild, C. C., and Zetter, B. R. (1979) Long-term culture of capillary endothelial cells. Proc. Natl. Acad. Sci. (USA) 76, 5217ā€“5221.

    ArticleĀ  CASĀ  Google ScholarĀ 

  9. Gitlin, J. D. and Dā€²Amore, P. A. (1983) Culture of retinal capillary cells using selective growth media. Microvasc. Res. 26, 74ā€“80.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  10. Herman, I. M. and Dā€²Amore, P. A. (1985) Microvascular pericytes contain muscle and non-muscle actins. J. Cell. Biol. 101, 43ā€“52.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  11. Nayak, R. C., Berman, A. B., George, K. L., Eisenbarth, G. S., and King, G. L. (1988) A monoclonal antibody (3G5) defined ganglioside antigen is expressed on the cell surface on microvascular pericytes. J. Exp. Med. 167, 1003ā€“1015.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  12. Davies, P., Smith, B. T., Maddalo, F. B., Langleben, D., Tobias, D., Fujiwara, K., and Reid, L. (1987) Characteristics of lung pericytes in culture including their growth inhibition by endothelial substrate. Microvasc. Res. 33, 300ā€“314.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  13. Carson, M. P. and Haudenschild, C. C. (1986) Microvascular endothelium and pericytes: High yield, low passage cultures. In Vitro Cell Dev. Biol. 22, 344ā€“354.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  14. Challier, J. C., Kacemi, A., and Olive, G. (1995) Mixed culture of pericytes and endothelial cells from fetal microvessels of the human placenta. Cell Mol. Biol. 41, 233ā€“241.

    CASĀ  PubMedĀ  Google ScholarĀ 

  15. Herman, I. M., Newcomb, P. M., Coughlin, J. E., and Jacobson, S. (1987) Characterization of microvascular cell cultures from normotensive and hypertensive rat brains: pericyte-endothelial cell interactions in vitro. Tissue & Cell 19, 197ā€“206.

    ArticleĀ  CASĀ  Google ScholarĀ 

  16. Burke, J. M., Skumatz, C. M., Irving, P. E., and McKay, B. S. (1996) Phenotypic heterogeneity of retinal pigment epithelial cells in vitro and in situ. Cur. Eye Res. 62, 63ā€“73.

    ArticleĀ  CASĀ  Google ScholarĀ 

  17. Newcomb, P. M. and Herman, I. M. (1993) Pericyte growth and contractile phenotype: Modulation by endothelial-synthesized matrix and comparison with aortic smooth muscle. J. Cell Phys. 155, 385ā€“393.

    ArticleĀ  CASĀ  Google ScholarĀ 

  18. Shepro, D. and Morel, N.M.L. (1993) Pericyte physiology. FASEB J. 7, 1031ā€“1038.

    CASĀ  PubMedĀ  Google ScholarĀ 

  19. Tassignon, M.-J., Stempels, N., Nguyen-Legros, J., De Wilde, F., and Brihaye, M. (1991) The effect of wavelength on glial fibrillary acidic protein immunoreactivity in laser-induced lesions in rabbit retina. Graefe ā€²s Arch. Clin. Exp. Ophthal. 22, 380ā€“388.

    ArticleĀ  Google ScholarĀ 

  20. Levy, R., Czernobilsky, B., and Geiger, B. (1988) Subtyping of epithelial cells of normal and metaplastic human uterine cervix, using polypeptide-specific cytokeratin antibodies. Differentiation 39, 185ā€“196.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  21. Mattey, D. L., Nixon, N., Wynn-Jones, C., and Dawes, P. T. (1993) Demonstration of cytokeratin in endothelial cells of the synovial microvasculature in situ and in vitro. Brit. J. Rheum. 32, 676ā€“682.

    ArticleĀ  CASĀ  Google ScholarĀ 

  22. Anderson, D. R. and Davis, E. B. (1996) Glaucoma, Capillaries and Pericytes: II. Identification and characterization of retinal pericytes in culture. Ophthalmologia 210, 263ā€“268.

    ArticleĀ  CASĀ  Google ScholarĀ 

  23. Gorfien, S., Spector, A., DeLuca, D., and Weiss, S. (1993) Growth and physiological functions of vascular endothelial cells in a new serum-free medium (SFM). Exp. Cell Res. 206, 291ā€“301.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  24. Lee, C. S., Patton, W. F., Chung-Welch, N., Chiang, E. T., Spofford, K. H., and Shepro, D. (1998) Selective propagation of retinal pericytes in mixed microvascular cell cultures using L-leucine-methyl ester. Biotechniques 25, 482ā€“494.

    CASĀ  PubMedĀ  Google ScholarĀ 

  25. Helmbold, P., Nayak, R. C., Holzhausen, H. J., Marsch, W. C., and Herman, I. M. Isolation of human dermal microvascular pericytes and identification in culture. Microvasc. Res. (in press).

    Google ScholarĀ 

  26. Helmbold, P., Wohlrab, J., Marsch W. C., and Nayak, R. C. Human dermal pericytes express 395 ganglioside: a new approach for microvessel histology. J. Curtaneous Pathol. (in press).

    Google ScholarĀ 

  27. Herman, I. M. (1988) Developing probes and methods for morphological and biochemical analyses of cytoskeletal elements in vascular cells. CRC Crit. Rev. Anatom. Sci. 1, 133ā€“148.

    Google ScholarĀ 

  28. Voyta, J. C., Via, D. P., Butterfield, C. E., Zettner, B. R. (1984) Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein. J. Cell Biol. 99, 2034ā€“2040.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

  29. Herman, I. M. and Dā€²Amore, P. A. (1985) Microvascular pericytes contain muscle and non-muscle actions. J. Cell Biol. 101, 43ā€“52.

    ArticleĀ  CASĀ  PubMedĀ  Google ScholarĀ 

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Ā© 2001 Humana Press Inc.

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Nayak, R.C., Herman, I.M. (2001). Bovine Retinal Microvascular Pericytes. In: Murray, J.C. (eds) Angiogenesis Protocols. Methods in Molecular Medicineā„¢, vol 46. Humana Press. https://doi.org/10.1385/1-59259-143-4:247

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  • DOI: https://doi.org/10.1385/1-59259-143-4:247

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-698-7

  • Online ISBN: 978-1-59259-143-5

  • eBook Packages: Springer Protocols

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