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Dryopteris dilatata leaf extract ameliorates streptozotocin-induced diabetic nephropathy in male Wistar rat

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Abstract

Background

Diabetic nephropathy (DN) is a serious consequence of diabetes mellitus (DM), and it is linked to higher morbidity and mortality in diabetic patients. The quest for cheap therapeutic strategy with lesser side effects remains a major health concern. However, Dryopteris dilatata is a commonly found flavonoid-rich plant with plethora of therapeutic potentials. This study investigated the effect of methanol extract of D. dilatata (MEDd) on streptozotocin-induced diabetic nephropathy in male Wistar rat.

Methods

Animals were randomly selected into five groups (n = 5) and were treated as follows; group 1 received distilled water (10 mL/kg), group 2 received only STZ (60 mg/kg), groups 3 and 4 received STZ then 400 and 800 mg/kg of MEDd, respectively, while group 5 received STZ then pioglitazone (10 mg/kg). Following 14 days of treatment, animals were euthanized, and blood as well as pancreas and kidney tissues were collected for further studies.

Results

Our results revealed that MEDd significantly reduced STZ-induced hyperglycemia in diabetic rats. Markers of oxidative injury (MDA, nitrite, and GSH) were also significantly ameliorated in the pancreas and kidney of the diabetic rats following treatment with MEDd.

However, renal function markers (creatinine and urea) were significantly attenuated with marked decreased in organ weight in the diabetic rats after treatment with MEDd. Also, serum insulin and corticosterone levels were restored following MEDd treatment.

Conclusion

Methanol extract of D. dilatata demonstrated anti-diabetogenic and reno-protective potential by enhancing in vivo reno-pancreatic antioxidant defense system.

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Data availability

This manuscript includes all of the study’s data.

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Acknowledgements

The technical knowledge of Divic Specialist Diagnostic Laboratory, Port Harcourt, Rivers State, Nigeria, is gratefully acknowledged by the authors.

Funding

The study was entirely self-funded by the authors.

Author information

Authors and Affiliations

  1. Department of Physiology, PAMO University of Medical Sciences, Port Harcourt, Nigeria

    Jerome Ndudi Asiwe

  2. Department of Physiology, Delta State University, Abraka, Nigeria

    Jerome Ndudi Asiwe, Bartholomew Chukwuebuka Nwogueze & Tarela Melish Elias Daubry

  3. Department of Pharmacology, Delta State University, Abraka, Nigeria

    Emuesiri Goodies Moke

  4. Department of Anatomy, University of Port Harcourt, Port Harcourt, Nigeria

    Nicholas Asiwe

  5. Department of Family Medicine, Delta State University, Abraka, Nigeria

    Godwin D. Yovwin

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  1. Jerome Ndudi Asiwe
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Contributions

The study was conceptualized by Jerome Ndudi Asiwe; the experimental assay was managed by Emuesiri Goodies Moke, Bartholomew Chukwuebuka Nwogueze, Daubry Tarela Mellish Elias, and Godwin D Yovwin; and the statistical analysis as well as manuscript writing was done by Jerome Ndudi Asiwe and Nicholas Asiwe.

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Correspondence to Jerome Ndudi Asiwe.

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The authors declare no competing interests.

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The authors declare no competing interests.

Human and animal rights

The PAMO University of Medical Science research ethics committee (PUMS-AREC/2021/067) approved the study, and the procedure closely complied with the ARRIVE recommendations as outlined in the 1996 revision of NIH Publication No. 85–23.

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Asiwe, J.N., Moke, E.G., Asiwe, N. et al. Dryopteris dilatata leaf extract ameliorates streptozotocin-induced diabetic nephropathy in male Wistar rat. Nutrire 48, 1 (2023). https://doi.org/10.1186/s41110-022-00186-4

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