Abstract
In this scientific work, organometric, histological, immunomorphologic, and morphometric parameters of the cerebral cortex of 219 corpses of people (108 men and 111 women) of young and old age have been studied. A comparative analysis of the parameters of bulb-shaped neurons in these ages revealed a decrease of their height and width. It was found that the distance between nerve-cell bodies of the ganglionic layer increases with age, which is obviously associated with disorganization progression and the death of bulbshaped neurons. An increase in the number of immunopositive astrocytes for the glial fibrillary acidic protein, protide S-100, and vimentin in the granular layer and the molecular layer of the cerebral cortex and decline in the number of immunopositive bulb-shaped neurons for neuron-specific enolase and immunonegative for the S-100 protein and vimentin that can be regarded as a manifestation of neurodegeneration. Using immunohistochemical methods in research allows a more nuanced approach to the issues of morphological assessment of the cerebral cortex of elderly people and makes it possible to obtain more objective and complete information on postnatal morphogenesis.
Similar content being viewed by others
References
Avrushchenko, M.Sh. and Afanas’eva, A.V., Change of cerebellar Purkinie cell population during postresuscitative period after blood circulation termination, Nauchno-prakt. konf. molodykh uchenykh “Sovremennye metody diagnostiki i lecheniya v reanimatologii” (Sci.-Pract. Conf. of Young Scientists “Modern Methods of Diagnostics and Cure in Resuscitation”), Moscow, 2009, pp. 5–7.
Avrushchenko, M.Sh., Ostrova, I.V., and Volkov, A.V., Postresuscitation changes in the expression of glial neurotrophic factor (GDNF): relationship with damage of cerebellar Purkinje cells (experimental study), Obshch. Reanimatol., 2014, vol. 10, no. 5, pp. 59–68.
Bazhanova, E.D., Anisimov, V.N., Sukhanov, D.S., and Teplyi, D.L., A comparative study of the effect of drugs for improvement of brain metabolism (angiogen, cytoflavin) on apoptosis of neurons and brain cortex function in aging experiment, Eksper. Klin. Farmakol., 2015, vol. 78, no. 2, pp. 3–9.
Bazhanova, E.D., Kozlova, Y.O., Anisimov, V.N., et al., Pharmacological correction of the apoptosis level in cortical neurons of ageing HER2/neu transgenic mice, J. Evol. Biochem. Physiol., 2016, vol. 52, no. 1, pp 64–73.
Barkhatova, V.P., Neurotransmitter organization and functional significance of the cerebellum, Ann. Klin. Eksper. Nevrol., 2010, vol. 4, no. 3, pp. 44–49.
Blinov, D.V. and Terent’ev, A.A., Characterization of biochemical markers of blood-brain-barrier permeability and the functioning of the central nervous system, Neurochem. J., 2013, vol. 7, no. 3, pp. 159–170.
Gomazkov, O.A., Cellular and molecular principles of brain aging, Usp. Sovrem. Biol., 2012, vol. 132, no. 2, pp. 141–154.
Derbeneva, O.A., Clinical role of S-100 protein as a marker of acute cerebral damage, Med. Obraz. Sib., 2013, no. 2, pp. 14.
Zhukova, I.A., Alifirova, V.M., and Zhukova, N.G., Neurospecific enolase as nonspecific marker of neurodegenerative process, Byull. Sib. Med., 2011, no. 2, pp. 15–21.
Zinov’eva, G.A., Aging of organism and imbalance, Klin. Nevrol., 2014, no. 4, pp. 37–45.
Kalinichenko, S.G. and Motavkin, P.A., Kora mozzhechka (The Cerebellar Cortex), Moscow: Nauka, 2005.
Kirik, O.V., S-100 protein, Morfologiya, 2007, no. 5, pp. 23–27.
Petrov, S.V. and Kiyasov, A.P., Immunogistokhimicheskaya diagnostika opukholei cheloveka: rukovodstvo dlya vrachei-morfologov (Immunohistochemical Diagnostics of Human Tumors: Manual for Physicians-Morphologists), Kazan: Dom Pechati, 1998.
Razumnikov, O.M., The pattern of brain aging and activation methods of compensatory resources, Usp. Fiziol. Nauk, 2015, vol. 46, no. 2, pp. 3–16.
Chekhonin, V.P., Shepeleva, I.I., and Gurina, O.I., Disturbances in the expression of neuronal cell adhesion proteins NCAM. Clinical aspects, Neurochem. J., 2008, vol. 2, no. 4, pp. 239–251.
Damodaran, T.V., Bilska, M.A., Rahman, A.A., and Abou-Donia, M.B., Sarin causes early differential alteration and persistent overexpression in mRNA coding for glial fibrillary acidic protein (GFAP) and vimentin genes in the central nervous system of rats, Neurochem. Res., 2002, vol. 27, no. 5, pp. 407–415.
Guidotti, A., Pesold, Ch., and Costa, E., New neurochemical markers for psychosis: a working hypothesis of their operation, Neurochem. Res., 2000, vol. 25, nos. 9–10, pp. 1207–1218.
Levin, E.C., Acharya, N.K., Sedeyn, J.C., et al., Neuronal expression of vimentin in the Alzheimer’s disease brain may be part of a generalized dendritic damageresponse mechanism, Brain Res., 2009, vol. 1298, no. 10, pp. 194–207.
Saleh, A., Sallam, K., Abadier, M., and Al-Kholy, A., Serum S-100 B and neuron-specific enolase as predictors of the neurologic disability status after traumatic brain injury, Egypt J. Neurol. Psychiatry Neurosurg., 2007, vol. 44, no. 1, pp. 143–156.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © I.A. Balandina, L.M. Zheleznov, A.A. Balandin, P.V. Kosareva, D.V. Borodulin, D.G. Amarantov, 2016, published in Uspekhi Gerontologii, 2016, Vol. 29, No. 4, pp. 670–675.
Rights and permissions
About this article
Cite this article
Balandina, I.A., Zheleznov, L.M., Balandin, A.A. et al. Morphological features of structural organization of the cerebellum cortex in old age. Adv Gerontol 7, 90–94 (2017). https://doi.org/10.1134/S2079057017010027
Published:
Issue Date:
DOI: https://doi.org/10.1134/S2079057017010027