Abstract
In this study, we evaluated the antitumor activity of a gene therapy complex in which the tumor-specific control of the expression of the effector suicide gene FCU1 was performed using a two-vector system based on the site-specific Cre–LoxP recombinase system. The complex of interest showed a high therapeutic potential in a mouse colon adenocarcinoma model.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Erbs, P., Regulier, E., Kintz, J., Leroy, P., Poitevin, Y., Exinger, F., Jund, R., and Mehtali, M., In vivo cancer gene therapy by adenovirus-mediated transfer of a bifunctional yeast cytosine deaminase/uracil phosphoribosyltransferase fusion gene, Cancer Res., 2000, vol. 60, pp. 3813–3822.
Graepler, F, Lemken, M.L., Wybranietz, W.A., Schmidt, U., Smirnow, I., Gross, C.D., Spiegel, M., Schenk, A., Graf, H., Lauer, U.A., Vonthein, R., Gregor, M., Armeanu, S., Bitzer, M., and Lauer, U.M., Bifunctional chimeric SuperCD suicide gene—YCD: YUPRT fusion is highly effective in a rat hepatoma model, World J. Gastroenterol., 2005, vol. 11, pp. 6910–6919.
Farokhimanesh, S., Rahbarizadeh, F., Rasaee, M.J., Kamali, A., and Mashkani, B., Hybrid promoters directed tBid gene expression to breast cancer cells by transcriptional targeting, Biotechnol. Prog., 2010, vol. 26, pp. 505–511.
Wirth, T., Zender, L., Schulte, B., Mundt, B., Plentz, R., Rudolph, K.L., Manns, M., Kubicka, S., and Kühnel, F., A telomerase-dependent conditionally replicating adenovirus for selective treatment of cancer, Cancer Res., 2003, vol. 63, pp. 3181–3188.
Kuzmin, D.V., Vinogradova, T.V., Kopantzev, E.P., and Sverdlov, E.D., Cre–LoxP mediated strong enhancement of pBIRC5 promoter driven suicide of cancer cells with CD/UPRT and fluorocytosine, Open Gene Ther. J., 2010, vol. 3, pp. 31–39.
Alekseenko, I.V., Pleshkan, V.V., Kopantzev, E.P., Stukacheva, E.A., Chernov, I.P., Vinogradova, T.V., and Sverdlov, E.D., Activity of the upstream component of tandem TERT/survivin promoters depends on features of the downstream component, PLoS One, 2012, vol. 7, p. e46474.
Kostina, M.B., Sass, A.V., Stukacheva, E.A., Korobko, I.V., and Sverdlov, E.D., Enhanced vector design for cancer gene therapy with hierarchical enhancement of therapeutic transgene expression, Hum. Gene Ther. Methods, 2017, vol. 28, pp. 247–254.
Shepelev, M.V., Korobko, E.V., Georgiev, G.P., Sverdlov, E.D., and Korobko, I.V., Application of mRNA regulatory regions to improve tumor specificity of transgene expression, Cancer Gene Ther., 2011, vol. 18, pp. 682–684.
Alekseenko, I.V., Snezhkov, E.V., Chernov, I.P., Pleshkan, V.V., Potapov, V.K., Sass, A.V., Monastyrskaya, G.S., Kopantzev, E.P., Vinogradova, T.V., Khramtsov, Y.V., Ulasov, A.V., Rosenkranz, A.A., Sobolev, A.S., Bezborodova, O.A., Plyutinskaya, A.D., Nemtsova, E.R., Yakubovskaya, R.I., and Sverdlov, E.D., Therapeutic properties of a vector carrying the HSV thymidine kinase and GM-CSF genes and delivered as a complex with a cationic copolymer, J. Transl. Med., 2015, vol. 13, p. 78.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © O.A. Bezborodova, I.V. Alekseenko, E.R. Nemtsova, A.A. Pankratov, O.B. Filyukova, R.I. Yakubovskaya, M.B. Kostina, V.K. Potapov, E.D. Sverdlov, 2018, published in Doklady Akademii Nauk, 2018, Vol. 483, No. 3.
Rights and permissions
About this article
Cite this article
Bezborodova, O.A., Alekseenko, I.V., Nemtsova, E.R. et al. The Antitumor Efficacy of a Complex Based on Two-Vector System for Coexpression of the Suicide Gene Fcu1 and Cre Recombinase. Dokl Biochem Biophys 483, 326–328 (2018). https://doi.org/10.1134/S1607672918060091
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1607672918060091