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Antitumor efficacy of combined gene and radiotherapy in animals

  • Biochemistry, Biophysics, and Molecular Biology
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Abstract

Antitumor efficacy of the combined suicide gene therapy and radiotherapy was studied on the model of CT26 murine colon adenocarcinoma. CMV-FCU1-IRES-mGM-CSF-pGL3 construct with PEG-PEI-TAT (FCU1–mGM/5-FC) block copolymer as a vector was used for intratumoral administration. Tumors were irradiated with a single 5 Gy dose. The efficacy was evaluated according to the grade of tumor growth inhibition (T/C) and lifespan of the animals. Pronounced antitumor activity of the combined use of FCU1–mGM/5-FC system with radiotherapy on the background of prolonged lifespan and the synergism of the applied methods was revealed.

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Authors and Affiliations

  1. National Medical Research Radiological Center (NMRRC), Ministry of Healthcare of the Russian Federation, Obninsk, Kaluga oblast, Russia

    O. A. Bezborodova, E. R. Nemtsova, A. R. Gevorkov, A. V. Boyko, J. B. Venediktova, E. V. Khmelevskiy & R. I. Yakubovskaya

  2. Institute of Molecular Genetics, Russian Academy of Sciences, pl. Akademika Kurchatova 46, Moscow, 123182, Russia

    I. V. Alekseenko & E. D. Sverdlov

  3. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    I. V. Alekseenko, M. B. Kostina, G. S. Monastyrskaya & E. D. Sverdlov

Authors
  1. O. A. Bezborodova
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  2. E. R. Nemtsova
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  3. A. R. Gevorkov
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  4. A. V. Boyko
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  5. J. B. Venediktova
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  6. I. V. Alekseenko
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  7. M. B. Kostina
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  8. G. S. Monastyrskaya
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  9. E. D. Sverdlov
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  10. E. V. Khmelevskiy
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  11. R. I. Yakubovskaya
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Corresponding author

Correspondence to O. A. Bezborodova.

Additional information

Original Russian Text © O.A. Bezborodova, E.R. Nemtsova, A.R. Gevorkov, A.V. Boyko, J.B. Venediktova, I.V. Alekseenko, M.B. Kostina, G.S. Monastyrskaya, E.D. Sverdlov, E.V. Khmelevskiy, R.I. Yakubovskaya, 2016, published in Doklady Akademii Nauk, 2016, Vol. 470, No. 4, pp. 471–474.

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Bezborodova, O.A., Nemtsova, E.R., Gevorkov, A.R. et al. Antitumor efficacy of combined gene and radiotherapy in animals. Dokl Biochem Biophys 470, 345–348 (2016). https://doi.org/10.1134/S1607672916050112

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  • DOI: https://doi.org/10.1134/S1607672916050112

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