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Application of Monoclonal Antibodies and Phage Display Technology for YB-1 Protein Analysis

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Abstract

Monoclonal antibodies against the recombinant YB-1 protein were obtained and characterized. These antibodies are capable of specific detection of YB-1 protein in the enzyme-linked immunosorbent assay, Western blot analysis, and immunocytochemistry. Using phage display technology, a peptide interacting with the YB-1 protein was found. Based on monoclonal antibodies МАYB-1-2 and a peptide as part of the bacteriophage structural protein, a test system was developed for the quantitative determination of YB-1 protein in blood serum. The detection limit of the developed test system is 0.3 ng/mL.

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Funding

The work was financially supported by the Russian Foundation for Basic Research (project nos. 18-54-00033 Bel_a and 17-00-00394 KOMFI).

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Authors and Affiliations

  1. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia

    A. G. Laman, A. O. Shepelyakovskaya, F. A. Brovko, S. V. Sizova & V. A. Oleinikov

  2. Research Institute for Physical Chemical Problems, Belarusian State University, 220006, Minsk, Belarus

    M. V. Artemyev

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  1. A. G. Laman
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Correspondence to A. O. Shepelyakovskaya.

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This article does not contain any studies involving human participants performed by any of the authors. All experimental procedures in animals were carried out in accordance with the international, national, and/or institutional principles for the keeping and study of laboratory animals.

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Translated by G. Levit

Abbreviations: FITC, fluorescein isothiocyanate; МАYB-1, monoclonal antibodies against YB-1; scFv, single-chain variable fragment; PBS, phosphate buffered saline; PBS-T, PBS containing 0.1% Tween-20

Corresponding author: phone: +7(4967)73-08-53; e-mail: shepelyakovskaya@rambler.ru.

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Laman, A.G., Shepelyakovskaya, A.O., Brovko, F.A. et al. Application of Monoclonal Antibodies and Phage Display Technology for YB-1 Protein Analysis. Russ J Bioorg Chem 46, 43–51 (2020). https://doi.org/10.1134/S1068162020010033

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