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Monoclonal antibodies to botulinum neurotoxins of types A, B, E, and F

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Abstract

Mouse monoclonal antibodies to health-threatening botulinum neurotoxins (BoNTs) of types A, B, E, and F have been produced and characterized. The antibodies are capable of interacting with a toxin inside the respective natural toxic complex. A sandwich ELISA for the quantitative detection of botulotoxins has been developed on based on the antibodies. The detection limits of the test systems for BoNTs A, B, E, and F is 0.4, 0.5, 0.1, and 2.4 ng/ml, respectively. The assay quantitatively detects BoNTs in canned meat and vegetables. Two antibodies, BNTA-4.1 and BNTA-9.1, both separately and in combination, are capable of neutralizing the natural botulinum toxic complex of type A in vivo; a combination of antibodies neutralizes a higher dose of the toxin. It has been shown that the antibody BNTA-4.1 binds specifically to the light (catalytic) chain of the toxin, and the antibody BNTA-9.1 interacts with the heavy chain. We believe that monoclonal antibodies BNTA-4.1 and BNTA-9.1 hold promise for developing therapeutic antibodies to treat BoNT/A-caused botulism in an emergency.

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Abbreviations

BoNT/A, BoNT/B, BoNT/E, and BoNT/F:

botulinum neurotoxins of types A, B, E, and F

FCS:

fetal calf serum

PCR:

polymerase chain reaction

PBS:

phosphate-buffered saline

PBST:

PBS with 0.1% Tween-20

mABs:

monoclonal antibodies

MLD:

minimum lethal dose

ELISA:

enzyme-linked immunosorbent assay

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino Branch, Russian Academy of Sciences, pr. Nauki 6, Pushchino, Moscow oblast, 142290, Russia

    S. G. Abbasova, N. V. Rudenko & A. Yu. Gorokhovatskii

  2. Pushchino State University, Pushchino, Moscow oblast, 142290, Russia

    M. V. Kapralova

  3. Gamalei Research Institute of Microbiology and Epidemiology, Russian Academy of Medical Sciences, Moscow, Russia

    I. D. Vinogradova & Yu. V. Vertiev

  4. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia

    V. A. Nesmeyanov & E. V. Grishin

Authors
  1. S. G. Abbasova
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  2. N. V. Rudenko
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  3. A. Yu. Gorokhovatskii
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  4. M. V. Kapralova
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  5. I. D. Vinogradova
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  6. Yu. V. Vertiev
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  7. V. A. Nesmeyanov
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  8. E. V. Grishin
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Corresponding author

Correspondence to N. V. Rudenko.

Additional information

Original Russian Text © S.G. Abbasova, N.V. Rudenko, A.Yu. Gorokhovatskii, M.V. Kapralova, I.D. Vinogradova, Yu.V. Vertiev, V.A. Nesmeyanov, E.V. Grishin, 2011, published in Bioorganicheskaya Khimiya, 2011, Vol. 37, No. 3, pp. 344–353.

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Abbasova, S.G., Rudenko, N.V., Gorokhovatskii, A.Y. et al. Monoclonal antibodies to botulinum neurotoxins of types A, B, E, and F. Russ J Bioorg Chem 37, 307–315 (2011). https://doi.org/10.1134/S1068162011030022

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  • DOI: https://doi.org/10.1134/S1068162011030022

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