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Dominant phylotypes in the 16S rRNA gene clone libraries from bacterial mats of the Uzon caldera (Kamchatka, Russia) hydrothermal springs

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Abstract

In situ analysis of the 16S rRNA genes from bacterial mats of five hydrothermal springs (36–58°C) in the Uzon caldera (Kamchatka, Russia) was carried out using clone libraries. Eight clone libraries contained 18 dominant phylotypes (over 4–5%). In most clone libraries, the phylotype of the green sulfur bacterium Chlorobaculum sp. was among the dominant ones. The phylotypes of the green nonsulfur bacteria Chloroflexus and Roseiflexus and of purple nonsulfur bacteria Rhodoblastus, Rhodopseudomonas, and Rhodoferax were also among the dominant ones. Cyanobacteria were represented by one dominant phylotype in a single spring. Among nonphototrophic bacteria, the dominant phylotypes belonged to Sulfyrihydrogenibium sp., Geothrix sp., Acidobacterium sp., Meiothermus sp., Thiomonas sp., Thiofaba sp., and Spirochaeta sp. Three phylotypes were not identified at the genus level. Most genera of phototrophic and nonphototrophic organisms corresponding to the phylotypes from Uzon hydrotherms have been previously revealed in the hydrotherms of volcanically active regions of America, Asia, and Europe. These results indicate predominance of bacterial mats carrying out anaerobic photosynthesis in the hydrotherms of the Uzon caldera.

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Authors and Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, 142290, Russia

    V. N. Akimov & M. G. Shlyapnikov

  2. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia

    V. N. Akimov, O. A. Podosokorskaya & V. F. Gal’chenko

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  1. V. N. Akimov
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  2. O. A. Podosokorskaya
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  3. M. G. Shlyapnikov
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  4. V. F. Gal’chenko
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Correspondence to V. N. Akimov or V. F. Gal’chenko.

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Original Russian Text © V.N. Akimov, O.A. Podosokorskaya, M.G. Shlyapnikov, V.F. Gal’chenko, 2013, published in Mikrobiologiya, 2013, Vol. 82, No. 6, pp. 707–714.

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Akimov, V.N., Podosokorskaya, O.A., Shlyapnikov, M.G. et al. Dominant phylotypes in the 16S rRNA gene clone libraries from bacterial mats of the Uzon caldera (Kamchatka, Russia) hydrothermal springs. Microbiology 82, 721–727 (2013). https://doi.org/10.1134/S0026261713060027

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