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Callus induction and plantlet regeneration in ornamental Alocasia micholitziana

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Abstract

Callus induction from petiole explants has been achieved in Alocasia micholitziana `Green Velvet'. The highest percentage (71%) of explants inducing callus was obtained on MS medium supplemented with 0.5 μM 2,4-D and 0.5 μM kinetin in the dark after 4 months of culture. Shoots were regenerated at the highest frequency of 33.3% under light condition when 0.5 μM BA was added to MS medium with the average of 7.8±2.3 shoots per callus explant. The callus-derived shoots rooted on hormone free MS medium and within 4 weeks the plantlets were ready for acclimatization. The regenerated plants appeared morphologically similar to mother plants.

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Authors and Affiliations

  1. Laboratory of Horticultural Science, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashiku, Fukuoka, 812-8581, Japan

    Nguyen Thi Phuong Thao, Yukio Ozaki & Hiroshi Okubo

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  1. Nguyen Thi Phuong Thao
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  2. Yukio Ozaki
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  3. Hiroshi Okubo
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Correspondence to Nguyen Thi Phuong Thao.

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Thao, N.T.P., Ozaki, Y. & Okubo, H. Callus induction and plantlet regeneration in ornamental Alocasia micholitziana . Plant Cell, Tissue and Organ Culture 73, 285–289 (2003). https://doi.org/10.1023/A:1023025717271

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  • DOI: https://doi.org/10.1023/A:1023025717271

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