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Effects of different photobiomodulation therapy doses on cell viability after bacterial and ionizing radiation–induced stress: a pilot in vitro study

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Abstract

Purpose

Photobiomodulation therapy has proven to be effective in accelerating cell proliferation, migration, and transcription. The study aimed to analyze the cell viability effects of different parameters of PBMT in a cultured cell line of human gingival fibroblasts after bacterial and ionizing radiation–induced stress.

Methods

Explant technique was used to produce a primary cell culture. Cells were grown in Dulbecco’s modified Eagle’s medium with 10% fetal bovine serum until stressful condition induction with lipopolysaccharide of Escherichia coli, Porphyromonas gingivalis protein extract, and ionizing radiation. Laser irradiation was carried out in four sessions set with 660 nm wavelength, an output power of 30 mW and 40 mW and energy density of 2, 3, 4, and 5 J/cm2.

Results

After 24 h from the last laser irradiation session, the groups outputted in 30 mW of power maintained the cell viability while operating with 2, 4, and 5 J/cm2. However, 3 J/cm2 dose significantly decreased cell viability (p < 0.05). When the laser irradiation session was set in a higher power (40 mW), cell viability was reduced using 2, 3, and 5 J/cm2 doses, with statistical significance for 5 J/cm2 (p < 0.001). In addition, operating the same energy using lower power seems to be superior to a higher power, being statically significant for 5 J/cm2 dose (p < 0.001). This pattern followed with all different groups, except by 3 J/cm2.

Conclusions

The present study showed that delivering 2, 4, and 5 J/ cm2 of density of energy with 30 mW and more time of exposure presented better results on cell viability compared to the same density of energy with output power of 40 mW. Further studies comparing density energy should be conducted.

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Funding

This study received financial support from DGP – UnB (Graduate Dean of the University of Brasilia) no. 0004/2021 in the name of J. Amorim dos Santos. The authors disclosed receipt of the following financial support for the research, authorship, and/or publication: M.M. Monteiro and J. Amorim dos Santos are supported by CAPES (Coordination for the Improvement of Higher Education Personnel), Brazil. V. Paiva Barbosa and E.N.S. Guerra are supported by the CNPq (National Council for Scientific and Technological Development), Ministry of Education, Brazil.

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Authors and Affiliations

  1. Laboratory of Oral Histopathology, Health Sciences Faculty, University of Brasília, Asa Norte, Brasília, DF, 70910-900, Brazil

    Mylene Martins Monteiro, Juliana Amorim dos Santos, Victor Paiva Barbosa & Eliete Neves Silva Guerra

  2. Stomatology Department, School of Dentistry, University of São Paulo, Sao Paulo, Brazil

    Camila de Barros Gallo

  3. Post Graduation Program, Ibirapuera University (UNIB), Sao Paulo, SP, Brazil

    Márcia Martins Marques

Authors
  1. Mylene Martins Monteiro
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  2. Juliana Amorim dos Santos
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  3. Victor Paiva Barbosa
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  4. Camila de Barros Gallo
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  5. Márcia Martins Marques
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  6. Eliete Neves Silva Guerra
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Contributions

All the authors contributed to the study’s conception and design. Material preparation, data collection, and analysis were performed by Mylene Martins Monteiro, Juliana Amorim dos Santos, and Victor Paiva Barbosa. The first draft of the manuscript was written by Mylene Martins Monteiro and Juliana Amorim dos Santos, and all the authors commented on previous versions of the manuscript. All the authors read and approved the final manuscript.

Corresponding author

Correspondence to Juliana Amorim dos Santos.

Ethics declarations

Ethics approval

This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Human Research Ethics Committee of the Health Sciences College of the University of Brasilia (CAAE No. 78679717.6.0000.0030).

Consent to participate

Informed consent was obtained from all individual participants that donated gingival fragments in the study.

Conflict of interest

The authors declare no conflict of interest.

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Monteiro, M.M., Amorim dos Santos, J., Paiva Barbosa, V. et al. Effects of different photobiomodulation therapy doses on cell viability after bacterial and ionizing radiation–induced stress: a pilot in vitro study. Laser Dent Sci 6, 205–210 (2022). https://doi.org/10.1007/s41547-022-00162-1

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  • DOI: https://doi.org/10.1007/s41547-022-00162-1

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