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Therapeutic Efficacy of Subcutaneous and Intraperitoneal Injections of a Single Dose of Human Umbilical Mesenchymal Stem Cells in Acute and Chronic Colitis in a Mouse Model

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Abstract

Purpose

Intraperitoneal (IP) injection of mesenchymal stem cells (MSCs) has been reported to treat colitis in mice. Complications such as abdominal organ injury and infection are significant concerns. We studied a single injection of human umbilical cord MSCs (hUCMSCs) via the subcutaneous (SC) and IP routes in mice with colitis.

Methods

Male C57BL/6Ncrl mice were divided into control, phosphate-buffered saline (PBS), hUCMSCs SC, and hUCMSCs IP injection groups. Colitis was induced by 3% dextran sulfate sodium (DSS). The disease activity index (DAI), colon length, histology, inflammation score, cytokine and chemokine staining and in vivo stem cell images were recorded.

Results

The DAI in the SC group was significantly lower than that in the IP group during late acute colitis. The colon was significantly shorter, and the colon inflammation score was significantly higher in the PBS group than the control group. There were no significant differences in the colon length and inflammation score between the control group and the SC and IP groups. The expressions of IL17A and Gro-α decreased in the SC group compared with those in the IP group on the 8th and 25th days. hUCMSCs via SC injection accumulated in the subcutaneous tissue to the 25th day.

Conclusion

hUCMSCs via SC and IP injection reduced DSS-induced acute colitis and decreased progression to chronic colitis. The anti-inflammatory effects of hUCMSCs were better in the SC injection group than the IP injection group. In clinical practice in humans, SC injection of hUCMSCs is relatively safer and more convenient than IP injection.

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Acknowledgements

This work was supported by grants from Taoyuan General Hospital, Taiwan (PTH10708 and PTH10709).

Funding

This study was funded by Taoyuan General Hospital, Taiwan (Grant Number: PTH10708 and PTH10709).

Author information

Authors and Affiliations

  1. Department of Urology, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, 33004, Taiwan

    Yu-Lung Chang & Sheng-Ping Lee

  2. Department of Biomedical Engineering, College of Engineering, Chung Yuan Christian University, Chungli, 32023, Taiwan

    Yu-Lung Chang, Xiu-Fang Lin & Ming-Fa Hsieh

  3. Department of Urology, School of Medicine, National Yang-Ming University, Taipei, 11221, Taiwan

    Yu-Lung Chang

  4. Translational Medicine Center, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, 33004, Taiwan

    Yu-Lung Chang, Huei-Yu Lo, Shun-Ping Cheng, Kuo-Ting Chang & Chin-Kan Chan

  5. Department of Rehabilitation, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, 33004, Taiwan

    Huei-Yu Lo & Shun-Ping Cheng

  6. Department of Chemistry, Chung Yuan Christian University, Chungli, 32023, Taiwan

    Huei-Yu Lo

  7. Division of Microbiology, Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, 33302, Taiwan

    Jason Ma & Ming-Ling Kuo

  8. Department of Microbiology and Immunology, College of Medicine, Chang Gung University, Taoyuan, 33302, Taiwan

    Ming-Ling Kuo

  9. Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, Taoyuan, 33302, Taiwan

    Ming-Ling Kuo

  10. Department of Pediatrics, Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, 33302, Taiwan

    Chin-Kan Chan

  11. Department of Biotechnology, School of Health Technology, Ming Chuan University, Taoyuan, 33347, Taiwan

    Chin-Kan Chan

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  1. Yu-Lung Chang
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Correspondence to Ming-Fa Hsieh or Chin-Kan Chan.

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Ethical Approval

This work was approved by the institutional review board of Taoyuan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan (IRB Number: TYGH104043). The research was conducted in accordance with the Helsinki Declaration. All applicable international, national, and institutional guidelines for the care and use of animals were followed. All animal experiments were performed in accordance with the relevant guidelines and regulations of the Animal Ethics Committee of Chung Yuan Christian University (IACUC Approval Number: 106019) accredited for laboratory animal care by the Ministry of Health and Welfare of Taiwan, Republic of China.

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Chang, YL., Lo, HY., Cheng, SP. et al. Therapeutic Efficacy of Subcutaneous and Intraperitoneal Injections of a Single Dose of Human Umbilical Mesenchymal Stem Cells in Acute and Chronic Colitis in a Mouse Model. J. Med. Biol. Eng. 40, 82–90 (2020). https://doi.org/10.1007/s40846-019-00494-7

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  • DOI: https://doi.org/10.1007/s40846-019-00494-7

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