Abstract
Purpose
To assess the plasma oxysterol species 7-ketocholesterol (7-Kchol) and cholestane-3β,5α,6β-triol (chol-triol) as biomarkers of oxidative stress in type 1 and type 2 diabetes mellitus (DM).
Methods
In total, 26 type 1 and 80 type 2 diabetes patients, along with 205 age- and gender-matched healthy controls, were included in this study. Oxysterols were quantified by liquid chromatography coupled with tandem mass spectrometry and N,N-dimethylglycine derivatization. Correlations between oxysterols and clinical/biochemical characteristics of the diabetes patients, and factors affecting 7-Kchol and chol-triol, were also determined.
Results
Plasma 7-Kchol and chol-triol levels were significantly higher in type 1 and type 2 diabetes patients compared to healthy controls (P < 0.001). Significant positive correlations were observed between oxysterol levels and levels of glycated hemoglobin (HbA1c), glucose, serum total cholesterol, low-density lipoprotein, very-low-density lipoprotein, and triglycerides, as well as the number of coronary risk factors. Statins, oral hypoglycemic agents, and antihypertensive agents reduced the levels of oxysterols in type 2 diabetes patients. Statin use, HbA1c levels, and the number of coronary risk factors accounted for 98.8% of the changes in 7-Kchol levels, and total cholesterol, smoking status, and the number of coronary risk factors accounted for 77.3% of the changes in chol-triol levels in type 2 diabetes patients.
Conclusions
Plasma oxysterol levels in DM, and particularly type 2 DM, may yield complementary information regarding oxidative stress for the clinical follow-up of diabetes patients, especially those with coronary risk factors.
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Funding
This study was supported by a grant from Hacettepe University Scientific Research Projects Coordination Unit (Project No: THD 201610908), Ankara, Turkey.
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Conceived, designed the study IL, AG. Wrote the paper IL, AS. Evaluated the patients SNS, AG. Performed the experiments AS, IL. Analyzed the data SK, AS, IL, AG, SNS. Contributed reagents/materials IL, FA.
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On behalf of all authors, the corresponding author states that there is no conflict of interest.
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The study protocol adhered to the Declaration of Helsinki Guidelines and was approved by the Ethics Committee of Hacettepe University (GO 15/662).
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Informed consent was obtained from each study participant.
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Samadi, A., Gurlek, A., Sendur, S.N. et al. Oxysterol species: reliable markers of oxidative stress in diabetes mellitus. J Endocrinol Invest 42, 7–17 (2019). https://doi.org/10.1007/s40618-018-0873-5
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DOI: https://doi.org/10.1007/s40618-018-0873-5