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Oral administration of nano-tyrosol reversed the diabetes-induced liver damage in streptozotocin-induced diabetic rats

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Abstract

Objectives

The present study was designed to evaluate the effects of Tyrosol and Nano-tyrosol on the cellular arrangement, collagen disposition, protein level of insulin receptor (INSR), and superoxide dismutase (SOD) activity in both control and streptozotocin-induced diabetic rats.

Methods

Type 2 Diabetes (T2D) was induced in rats by a single intraperitoneal injection of streptozotocin (50 mg/kg). Experimental rats were administered Tyrosol and Nano-tyrosol 1 ml intra-gastrically at a dose of 20 mg/kg once a day for 30 days. Then, rats were sacrificed according to ethical principles. Livers were removed and processed for histological studies using the paraffin technique. Furthermore, non-paraffin sections were used for the INSR-1 western blot technique.

Results

At the end of the experiments, the rats in diabetic control and plain niosome groups exhibited a significant increase in collagen disposition (p < 0.001), and apoptotic cells (p < 0.001), as well as decreased total protein levels of INSR (p < 0.001), and SOD activity (p < 0.001) in the hepatic cells. Oral administration of Tyrosol and Nano-tyrosol to diabetic rats reversed all the above-mentioned parameters to near normal levels (p < 0.001). Nano-tyrosol showed the highest significant effect rather than Tyrosol.

Conclusion

The results of the present study suggested the beneficial effects of Tyrosol and especially Nano-tyrosol on decreasing the adverse effects of diabetes.

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Acknowledgements

The authors thank the IAUTMU’s Herbal pharmacology research center and animal room for their support and equipment.

Funding

This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

Author information

Authors and Affiliations

  1. Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

    Nima Jafari-Rastegar, Haniyeh-Sadat Hosseininia, Elahe Jalilvand & Maryam Naseroleslami

  2. Herbal pharmacology research center, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

    Nima Jafari-Rastegar, Haniyeh-Sadat Hosseininia & Elahe Jalilvand

  3. Cognitive and Neuroscience Research Center (CNRC), Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

    Fateme Khakpai

  4. Department of Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, P.O.Box: 193951495, Tehran, Iran

    Neda Mousavi-Niri

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  1. Nima Jafari-Rastegar
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Contributions

Jafari-Rastegar N., Hosseininia HS., and Jalilvand E. acquired the animal data. Khakpai F. and Naseroleslami N. wrote the manuscript and analyzed the data. Mousavi-Niri N. was responsible for the study design and interpretation of results.

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Correspondence to Neda Mousavi-Niri.

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Jafari-Rastegar, N., Hosseininia, HS., Jalilvand, E. et al. Oral administration of nano-tyrosol reversed the diabetes-induced liver damage in streptozotocin-induced diabetic rats. J Diabetes Metab Disord 22, 297–305 (2023). https://doi.org/10.1007/s40200-022-01133-w

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  • DOI: https://doi.org/10.1007/s40200-022-01133-w

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