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The Combination of Platelet Rich Plasma Gel, Human Umbilical Mesenchymal Stem Cells and Nanohydroxyapatite/polyamide 66 Promotes Angiogenesis and Bone Regeneration in Large Bone Defect

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

In the present study, a novel tissue engineering bone graft including platelet rich plasma gel (PRP gel), human umbilical mesenchymal stem cells (HUMSCs) and nanohydroxyapatite/polyamide 66 (nHA-PA66) was constructed. We explored whether the composite scaffolds could enhance the angiogenesis and bone repair capacity in rat femoral large bone defect (LBD). This study aimed to provide evidence for the clinical application of the composite scaffold in LBD treatment.

Methods:

PRP was prepared, the platelets and growth factors were measured. HUMSCs were isolated and identified. the osteogenic capacity of PRP in vitro was measured. Then HUMSCs-PRP-gel/nHA-PA66 composite scaffolds were synthesized and observed. The proliferation and osteogenesis differentiation of HUMSCs on the composite scaffold was measured. The angiogenic capacity of PRP in vitro was measured by capillary-like tube formation assay. Finally, the angiogenesis and bone repair capacity of the composite scaffolds was measured in rat LBD.

Results:

PRP contained high level of platelets and growth factors after activation, and promoted osteogenic and angiogenic differentiation in vitro. The HUMSCs-PRP-gel/nHA-PA66 composite scaffold was porosity and promoted the proliferation and osteogenesis differentiation of HUMSCs. At 12th weeks, more micro-vessels and new bone were formed around the composite scaffolds compared with other groups, the defect was almost repaired.

Conclusion:

Our study for the first time identified that the combination of PRP gel, HUMSCs and nHA-PA66 scaffold could significantly promote angiogenesis and bone regeneration in rat LBD, which may have implications for its further application in clinical LBD treatment.

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Acknowledgements

The authors wish to thank Professor Lei Yang from Harbin Medical University, China, for the technical help and helpful discussion. We also gratefully acknowledge the core facilities and software provided by the Orthopedic Diagnosis and Treatment Center of the First Affiliated Hospital of Harbin Medical University, China.

Funding

This work was funded by Heilongjiang Provincial Postdoctoral Science Foundation, China (No.LBH-Z18202).

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Author notes

  1. Wei Liu and Yong Huang are co-first authors.

Authors and Affiliations

  1. Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin, 150001, Heilongjiang, China

    Wei Liu, Teng Zeng, Jingzhe Wang, Dawei Wang & Xiaoyu Wang

  2. Department of Orthopedic Surgery, The Affiliated Hospital of Qinghai University, Xining, Qinghai, China

    Yong Huang

  3. Department of Orthopedic Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China

    Daqian Liu & Ang Li

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  1. Wei Liu
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Correspondence to Xiaoyu Wang.

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The animal studies were performed after receiving approval of the Institutional Animal Care and Use Committee (IACUC) in Harbin Medical University (IACUC no. 2022061)

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Liu, W., Huang, Y., Liu, D. et al. The Combination of Platelet Rich Plasma Gel, Human Umbilical Mesenchymal Stem Cells and Nanohydroxyapatite/polyamide 66 Promotes Angiogenesis and Bone Regeneration in Large Bone Defect. Tissue Eng Regen Med 19, 1321–1336 (2022). https://doi.org/10.1007/s13770-022-00471-3

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