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Intra-Articular Injection of miR-29a-3p of BMSCs Promotes Cartilage Self-Repairing and Alleviates Pain in the Rat Osteoarthritis

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

Stem cells intra-articular injection stagey indicated a potential therapeutic effect on improving the pathological progress of osteoarthritis (OA). However, the long-term effect of stem cells intra-articular injection on the cartilage regeneration remains unclear. miR-29a-3p is predicted to be a critical target for inhibiting insulin-like growth factor-1 expression and may aggravate the progression of OA.

Methods:

In this study, we investigated the therapeutic efficacy of intra-articular injection of bone marrow mesenchymal stem cells (BMSCs) transfected with miR-29a-3p inhibitor in OA.

Results:

miR-29a-3p inhibitor transfection did not influence cell viability of BMSCs, while the chondrogenic differentiation potential of BMSCs was significantly improved. Interestingly, intra-articular injection of BMSCs with miR-29a-3p inhibition significantly prevented articular cartilage degeneration by up-regulating the expression of Sox 9, Col-2a1, aggrecan and down-regulating the expression of matrix metalloproteinase, as well as relieved pain in OA.

Conclusion:

The double effects on cartilage protection and pain relief indicated a great potential of intra-articular injection of miR-29a-3p inhibitor-transfected BMSCs for the treatment of OA.

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Acknowledgement

This study has been supported by Suzhou People's Livelihood Science and Technology (Project No. SYS2020065).

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Author notes

  1. Qing Wang and Yong Chen have contributed equally to this work.

Authors and Affiliations

  1. Department of Orthopedics, Kunshan Affiliated Hospital of Nanjing University of Chinese Medicine, Kunshan, 215300, China

    Qing Wang, Yong Chen & Ji Chen

  2. Department of Orthopedics, Kunshan Hospital of Traditional Chinese Medicine, Kunshan, 215300, China

    Qing Wang, Yong Chen & Ji Chen

  3. Department of Orthopedics, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, 215000, China

    Xiaofeng Shen & Yuwei Li

Authors
  1. Qing Wang
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  2. Yong Chen
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  3. Xiaofeng Shen
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  4. Ji Chen
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  5. Yuwei Li
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Correspondence to Yuwei Li.

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Ethical Statement

All animal procedures were performed in accordance with the guidelines for Care and Use of Laboratory Animal Experience and approved by the Animal Care and Use Ethics Committee of Nanjing University of Chinese Medicine (no. ACU191101).

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Wang, Q., Chen, Y., Shen, X. et al. Intra-Articular Injection of miR-29a-3p of BMSCs Promotes Cartilage Self-Repairing and Alleviates Pain in the Rat Osteoarthritis. Tissue Eng Regen Med 18, 1045–1055 (2021). https://doi.org/10.1007/s13770-021-00384-7

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  • DOI: https://doi.org/10.1007/s13770-021-00384-7

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