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Implantation of Bone Marrow Mesenchymal Stem Cells into Small Intestinal Submucosa Improves Bile Duct Injury in Rabbits

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

BACKGROUND:

Bile duct injury (BDI), which may occur during cholecystectomy procedures and living-donor liver transplantation, leads to life-altering complications and significantly increased mortality and morbidity. Tissue engineering, as an emerging method, has shown great potential to treat BDI. Here, we aimed to explore the application of small intestinal submucosa (SIS) matrix composites with bone marrow mesenchymal stem cells (BMSCs) to treat BDI in a rabbit model.

METHODS:

Rabbit-derived BMSCs were used as seed cells. Porcine SIS was used as the support material. Five centimetres of the common bile duct was dissected, and 1/3–1/2 of the anterior wall diameter was transversely incised to construct the rabbit BDI model. Then, SIS materials without/with BMSCs were inserted into the common bile duct of the BDI rabbits. After 1, 2, 4, and 8 weeks of implantation, the common bile duct was removed. Haematoxylin and eosin (HE) staining was used to assess pathological alterations in the common bile duct, while immunohistochemical staining and western blotting were used to detect expression of the epithelial cell markers CK19 and E-cadherin. Scanning electron microscopy was used to evaluate BMSC growth.

RESULTS:

Compared with BMSCs alone, SIS-attached BMSCs had increased growth. HE staining showed that the injured bile duct healed well and that the complex gradually degraded as the time from implantation increased. Immunohistochemical staining and western blotting showed that compared with the control group, the in vivo complex group had significantly elevated expression levels of CK19 and E-cadherin.

CONCLUSION:

BMSC implantation into SIS could improve BDI in rabbits, which might have clinical value for BDI treatment.

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Acknowledgements

We thank The youth fund of Southwest Medical University (No. 2018-ZRQN-096) for providing support for the study.

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Author notes

  1. Ying Li and Piao Wang have contributed equally to this work.

Authors and Affiliations

  1. Department of Plastic and Burns Surgery, The Affiliated Hospital of Southwest Medical University, National Key Clinical Construction Specialty, Luzhou, 646000, Sichuan, People’s Republic of China

    Ying Li

  2. Department of General Surgery (Hepatobiliary Surgery), The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, Sichuan, People’s Republic of China

    Piao Wang, Xiao-dong Hu, Cheng Fang, Yu Gan, Fang-yi Peng, Xiao-li Yang, De Luo, Bo Li & Song Su

  3. Department of Orthopaedic, Hospital of Traditional Chinese Medicine, affiliated to Southwest Medical University, Luzhou, 646000, Sichuan, People’s Republic of China

    Jing-da Zeng

  4. Academician (Expert) Workstation of Sichuan Province, Luzhou, 646000, China

    Bo Li & Song Su

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  1. Ying Li
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  2. Piao Wang
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Correspondence to Bo Li or Song Su.

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This study was approved by the Institutional Review Board of the Southwest Medical University (SWMU) (No. SWMU20180306059).

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Li, Y., Wang, P., Hu, Xd. et al. Implantation of Bone Marrow Mesenchymal Stem Cells into Small Intestinal Submucosa Improves Bile Duct Injury in Rabbits. Tissue Eng Regen Med 18, 887–893 (2021). https://doi.org/10.1007/s13770-021-00351-2

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  • DOI: https://doi.org/10.1007/s13770-021-00351-2

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