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Study of Recellularized Human Acellular Arterial Matrix Repairs Porcine Biliary Segmental Defects

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Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

Background:

With the popularity of laparoscopic cholecystectomy, common bile duct injury has been reported more frequently. There is no perfect method for repairing porcine biliary segmental defects.

Methods:

After the decellularization of human arterial blood vessels, the cells were cultured with GFP+ (carry green fluorescent protein) porcine bile duct epithelial cells. The growth and proliferation of porcine bile duct epithelial cells on the human acellular arterial matrix (HAAM) were observed by hematoxylin–eosin (HE) staining, electron microscopy, and immunofluorescence. Then, the recellularized human acellular arterial matrix (RHAAM) was used to repair biliary segmental defects in the pig. The feasibility of it was detected by magnetic resonance cholangiopancreatography, liver function and blood routine changes, HE staining, immunofluorescence, real-time quantitative PCR (RT-qPCR), and western blot.

Results:

After 4 weeks (w) of co-culture of HAAM and GFP+ porcine bile duct epithelial cells, GFP+ porcine bile duct epithelial cells grew stably, proliferated, and fused on HAAM. Bile was successfully drained into the duodenum without bile leakage or biliary obstruction. Immunofluorescence detection showed that GFP-positive bile duct cells could still be detected after GFP-containing bile duct cells were implanted into the acellular arterial matrix for 8 w. The implanted bile duct cells can successfully resist bile invasion and protect the acellular arterial matrix until the newborn bile duct is formed.

Conclusion:

The RHAAM can be used to repair biliary segmental defects in pigs, which provides a new idea for the clinical treatment of common bile duct injury.

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Acknowledgements

The authors thank Doc. Li Li (Department of hepatobiliary surgery, Ganmei Hospital affiliated to Kunming Medical University, Kunming, Yunnan, 650500, China) for the critical comment and discussion on this study.

Funding

The study was supported by National Natural Science Foundation of China (No. 81560089) and Yunnan Province Health Science and Technology Project (No.2014NS198).

Author information

Authors and Affiliations

  1. Department of Hepatobillary Surgery, Ganmei Hospital Affiliated to Kunming Medical University, No. 1228 Beijing road, Panlong district, Kunming, Yunnan, 650500, China

    Wei Liu & Li Li

  2. Department II of Hepatobillary Surgery, The People’s Hospital of Chuxiong Yi Autonomous Prefecture, No. 318 Lucheng south road, Chuxiong, Yunnan, 675000, China

    Wei Liu

  3. Department I of Hepatobiliary Pancreas and Pancreas, Ganmei Hospital Affiliated to Kunming Medical University, No. 1228 Beijing road, Panlong district, Kunming, Yunnan, 650500, China

    Sheng-Ning Zhang

  4. Department II of Hepatobiliary Pancreas and Pancreas, Ganmei Hospital Affiliated to Kunming Medical University, No. 504, Xishan District, Kunming, Yunnan, 650500, China

    Zong-Qiang Hu

  5. Ganmei Hospital Affiliated to Kunming Medical University, No. 1228 Beijing road, Panlong district, Kunming, Yunnan, 650500, China

    Shi-Ming Feng

  6. Department of Radiology, Yunnan Cancer Hospital, No. 519 Kunzhou road, Xishan district, Kunming, Yunnan, 650500, China

    Zhen-Hui Li

  7. Department of General Surgery, Puer People’s Hospital, No. 44 Revitalization Avenue, Puer, Yunnan, 655000, China

    Shu-Feng Xiao

  8. Department of Cardiac Surgery, The People’s Hospital of Chuxiong Yi Autonomous Prefecture, No. 318 Lucheng south road, Chuxiong, Yunnan, 675000, China

    Hong-Shu Wang

Authors
  1. Wei Liu
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  2. Sheng-Ning Zhang
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  3. Zong-Qiang Hu
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  4. Shi-Ming Feng
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  5. Zhen-Hui Li
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  6. Shu-Feng Xiao
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  7. Hong-Shu Wang
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  8. Li Li
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Contributions

Wei Liu drafting the article, a substantial contribution to the conception and design of the study. Sheng-Ning Zhang, Zong-Qiang Hu, Shi-Ming Feng, Zhen-Hui Li, Shu-Feng Xiao, and Hong-Shu Wang performed the research and contribution to acquisition, interpretation, and analysis of data. Li Li revising the draft critically and final approval of the version to be published.

Corresponding author

Correspondence to Li Li.

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Conflicts of interest

The authors have no financial conflicts of interest.

Ethical statement

The study protocol was approved by the institutional review board of Ganmei Hospital affiliated to Kunming Medical University. Informed consent was confirmed by the Ganmei Hospital affiliated to Kunming Medical University. The animal studies were performed after receiving approval of the Ethics Committee of Ganmei Hospital affiliated to Kunming Medical University (2015-01).

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Cite this article

Liu, W., Zhang, SN., Hu, ZQ. et al. Study of Recellularized Human Acellular Arterial Matrix Repairs Porcine Biliary Segmental Defects. Tissue Eng Regen Med 16, 653–665 (2019). https://doi.org/10.1007/s13770-019-00212-z

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  • DOI: https://doi.org/10.1007/s13770-019-00212-z

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