Skip to main content
SpringerLink
Log in

Comparative study of antidiarrheal activity of methanol extracts from leaf and fruit of Pandanus odoratissimus Linn

  • Research Article
  • Published:
Oriental Pharmacy and Experimental Medicine Aims and scope Submit manuscript

Abstract

Pandanus odoratissimus, a perennial, large shrub or small tree, has multifarious medicinal uses. The purpose of the present study was to evaluate the antidiarrheal effects of methanol extracts obtained from leaf and fruit of P. odoratissimus. The methanol extracts from the leaf and fruit of P. odoratissimus were evaluated for the antidiarrheal activity in rats. The effects of the extracts on defecation, intestinal transit and intestinal fluid accumulation (enteropooling) were assessed in castor oil-induced diarrhea. Oral administration of leaf and fruit extracts at 200 and 400 mg/kg exhibited significant (P < 0.05–0.01) and dose-dependent antidiarrheal potential in castor oil-induced diarrhea. The diarrheal episode was inhibited by 34.30 % and 47.60 % by leaf extract, whereas 35.11 % and 47.15 % by fruit extract at the doses of 200 and 400 mg/kg respectively. The extracts were found to possess an antienteropooling in castor oil-induced experimental animals by reducing both weight and volume of intestinal content significantly and also decrease in intestinal transit comparable to that of standard drug, loperamide (5 mg/kg). These findings revealed that methanol extracts of leaf and fruit of P. odoratissimus possess significant antidiarrheal activities.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adkar PP, Jadhav PP, Ambavade SD, Shelke TT, Bhaskar VH (2014) Protective effect of leaf extract of Pandanus odoratissimus Linn on experimental model of epilepsy. Int J Nutr Pharmacol Neurol Dis 4:81–87

    Article  Google Scholar 

  • Ammon HV, Thomas PJ, Phillips SF (1974) Effects of oleic and ricinoleic acids on net jejunal water and electrolyte movement Perfusion studies in man. J Clin Investig 53:374–379

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Atta AH, Mouneir SM (2004) Antidiarrhoeal activity of some Egyptian medicinal plant extracts. J Ethnopharmacol 92:303–309

    Article  PubMed  Google Scholar 

  • Awe EO, Kolawole SO, Wakeel KO, Abiodun OO (2011) Antidiarrheal activity of Pyrenacantha staudtii Engl. (Icacinaceae) aqueous leaf extract in rodents. J Ethnopharmacol 137:148–153

    Article  PubMed  Google Scholar 

  • Awouters F, Neimegeers CJE, Lenaert FM, Janssen PAJ (1978) Delay of castor oil diarrhoea in rats: a new way to evaluate inhibitors of prostaglandin biosynthesis. J Pharm Pharmacol 30:41–45

    Article  CAS  PubMed  Google Scholar 

  • Beubler E, Juan H (1979) Effect of ricinoleic acid and other laxatives on net water flux and prostaglandin E release by the rat colon. J Pharm Pharmacol 31:681–685

    Article  CAS  PubMed  Google Scholar 

  • Chatterjee A, Sc P (2001) The Treatise on Indian medicinal plants, 6th edn. National Institute of Science Communication, New Delhi, pp 9–10

    Google Scholar 

  • Chilakwad SR, Manjunath KP, Akki KS, Savadi RV, Deshpande N (2008) Pharmacognostic and phytochemical investigation of leaves of Pandanus odoratissimus Linn.f. Anc Sci Life 28:3–6

    PubMed Central  PubMed  Google Scholar 

  • Chopra R, Nayar SL, Chopra IC (1996) Glossary of Indian Medicinal Plants, 1st edn. CSIR, New Delhi, pp 184–185

    Google Scholar 

  • El-Shaibany A (2014) Nocturnal enuresis, antioxidant and antimicrobial activities of Pandanus odoratissimus L. Peduncle. IJPSR 5:811–818

  • Ezeja IM, Ezeigbo II, Madubuike KG, Udeh NE, Ukweni IA, Akomas SC, Ifenkwe DC (2012) Antidiarrheal activity of Pterocarpus erinaceus methanol leaf extract in experimentally-induced diarrhea. Asian Pac J Trop Med 5(2):147–50

    Article  PubMed  Google Scholar 

  • Ilanchezhian R, Joseph RC (2010) Hepatoprotective and hepatocurative activity of the traditional medicine Ketaki (Pandanus odoratissimus Roxb.). Asian J Tradit Med 5:212–218

    Google Scholar 

  • Jong TT, Chau SW (1998) Antioxidative activities of constituents isolated from Pandanus odoratissimus. Phytochemistry 49:2145–2148

    Article  CAS  Google Scholar 

  • Kumar D, Kumar S, Kumar S, Singh J, Sharma C, Aneja KR (2010) Antimicrobial and preliminary phytochemical screening of crude leaf extract of Pandanus odoratissimus L. Pharmacologyonline 2:600–610

    Google Scholar 

  • Kusuma R, Reddy PV, Babu NB, Venkatesh S (2012) Phytochemical and phamacological studies of Pandanus odoratissimus Linn. Int J Res Phytochem Pharmacol 2:171–174

    CAS  Google Scholar 

  • Londonkar R, Kamble A (2009) Evaluation of free radical scavenging activity of Pandanus odoratissimus. Int J Pharmacol 5:377–380

    Article  Google Scholar 

  • Londonkar R, Kamble A (2011) Hepatotoxic and in-vivo antioxidant potential of Pandanus odoratissimus against carbon tetrachloride induced liver injury in rats. Orient Pharm Exp Med 11:229–234

    Article  Google Scholar 

  • Londonkar R, Kamble A, Reddy VC (2010) Anti-inflammatory activity of Pandanus odoratissimus extract. Int J Pharmacol 6:311–314

    Article  Google Scholar 

  • Longanga Otshudi A, Vercruysse A, Foriers A (2000) Contribution to the ethnobotanical, phytochemical and pharmacological studies of traditionally used medicinal plants in the treatment of dysentery and diarrhoea in Lomela area, Democratic Republic of Congo (DRC). J Ethnopharmacol 71:411–423

    Article  CAS  PubMed  Google Scholar 

  • Maikere-Faniyo R, Puyvelde VL, Mutwewingabo A, Habiyaremye FX (1989) Study of Rwandese medicinal plants used in the treatment of diarrhea. J Ethonopharmacol 26:101–109

    Article  CAS  Google Scholar 

  • Mascolo N, Izzo AA, Autore G, Barbato F, Capasso F (1994) Nitric oxide and castor oil-induced diarrhea. J Pharmacol Exp Ther 268:291–295

    CAS  PubMed  Google Scholar 

  • Nadkarni KM (2002) Indian Materia Medica, 1st edn. Bombay Popular Prakashan, Bombay, pp 894–895

    Google Scholar 

  • Nwafor PA, Okwuasaba FK, Binda LG (2000) Antidiarrhoeal and antiulcerogenic effects of methanolic extract of Asparagus pubescent root in rats. J Ethnopharmacol 72:421–427

    Article  CAS  PubMed  Google Scholar 

  • Ojewole JAO, Awe EOA, Agatha N (2009) Antidiarrhoeal activity of Hypoxis hemerocallidea Fisch. & C. A. Mey. (Hypoxidaceae) Corm (‘African potato’) aqueous extract in rodents. Phytother Res 23:965–971

    Article  PubMed  Google Scholar 

  • Palombo EA (2006) Phytochemicals from traditional medicinal plants used in the treatment of diarrhoea: modes of action and effects on intestinal function. Phytother Res 20:717–724

    Article  CAS  PubMed  Google Scholar 

  • Panigrahi BB, Panda PK, Patro VJ (2011) Antitumor and in-vivo antioxidant activities of Pandanus odoritissimus Linn. against ehrlich ascites carcinoma in swiss albino mice. IJPSRR 8:202–208

    Google Scholar 

  • Prajapati ND, Purohit SS, Sharmak A, Kumar T (2003) A handbook of medicinal plants, 1st edn. Jodhpur, Agrobios, pp 378–379

    Google Scholar 

  • Raju S, Subbaish NV, Reddy KS, Das A, Murugun KB (2011) Potential of Pandanus odoratissimus as a CNS depressant in swiss albino mice. Braz J Pharm Sci 47:629–634

    Article  CAS  Google Scholar 

  • Robert A, Nezamis JE, Lancaster C, Hanchar AJ, Klepper MS (1976) Enteropooling assay: a test for diarrhea produced by prostaglandins. Prostagladins 11:809–828

    CAS  Google Scholar 

  • Sasikumar JM, Jinu U, Shamna R (2009) Antioxidant activity and HPTLC analysis of Pandanus odoratissimus L. root. European J Biol Sci 1:17–22

    Google Scholar 

  • Thompson PM, Islam MA (eds) (2010) Environmental profile of St. Martin’s Island United Nations Development Programme, Dhaka, p 32

    Google Scholar 

  • Vaidyarathnam (1997) Indian Medicinal plants a compendium of 500 species. 4th ed, Varier’s PS, Aryavaidyasaia, Orent Longman Limited, Kattakkal, pp 206–210

  • Venkatesh S, Kusuma R, Sateesh V, Reddy MB, Mullangi R (2012) Antidiabetic activity of Pandanus odoratissimus root extract. Indian J Pharm Educ Res 46:340–345

    Google Scholar 

  • Wei LS, Musa N, Sengm CT, Wee W, Shazili NAM (2008) Antimicrobial properties of tropical plants against 12 pathogenic bacteria isolated from aquatic organisms. Afr J Biotechnol 7:2275–2278

    CAS  Google Scholar 

  • Zavala MA, Perez S, Perez C, Vargal R, Perez RM (1998) Antidiarrhoeal activity of Waltheria americana, Commelina coelestis and Alternanthera repens. J Ethnopharmacol 61:41–47

    Article  CAS  PubMed  Google Scholar 

  • Zhang X, Wu C, Wu H, Sheng L, Su Y, Zhang X, Luan H, Sun G, Sun X, Tian Y, Ji Y, Guo P, Xu X (2013) Anti-hyperlipidemic effects and potential mechanisms of action of the caffeoylquinic acid-rich Pandanus tectorius fruit extract in hamsters fed a high fat-diet. PLoS ONE 8(4):e61922

    Article  CAS  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgements

Authors are grateful to the Department of Pharmacy, International Islamic University Chittagong, Bangladesh for providing research facilities.

Conflict of Interest statement

We declare that we have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Pharmacy, Faculty of Science and Engineering, International Islamic University Chittagong, 154/A, College Road, Chittagong, 4203, Bangladesh

    Md Khalilur Rahman, Md Fokhrul Islam, Soumitra Barua, Md Masudur Rahman & Mohammed Abu Sayeed

Authors
  1. Md Khalilur Rahman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Md Fokhrul Islam
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Soumitra Barua
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Md Masudur Rahman
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mohammed Abu Sayeed
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Md Masudur Rahman.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Rahman, M.K., Islam, M.F., Barua, S. et al. Comparative study of antidiarrheal activity of methanol extracts from leaf and fruit of Pandanus odoratissimus Linn. Orient Pharm Exp Med 14, 363–367 (2014). https://doi.org/10.1007/s13596-014-0167-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13596-014-0167-1

Keywords

Search

Navigation