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Deriving stable tropical gynoecious inbred lines of slicing cucumber from American pickling cucumber using MABB

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Abstract

This study was conducted to develop inbred backcross lines (IBLs) in Indian slicing cucumber (Cucumis sativus L.) with the gynoecious trait. The F2 mapping population was developed using the Indian monoecious cultivar of light-green-fruited Pusa Uday as the recurrent parent and the American gynoecious pickling line G421 as a donor. Two markers, SSR13251 and SSR15516, were found to be closely linked to the F locus at 1.5 and 4.5 cM, respectively, by marker analysis of F2 individuals. The amplified product of 430 bp generated using a SCAR marker (CsACS1G) in gynoecious (MMFF) and sub-gynoecious (MMFf) plants of the F2 population confirmed the dominance of F alleles. The IBLs were developed by backcrossing Pusa Uday with BC1 and BC2 progenies followed by foreground and background selection of plants displaying the gynoecious habit in the background of Indian slicing cucumber. These IBLs could be utilized for the development of hybrids with higher yield, earliness, and quality.

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Data availability

The data generated during the current study is available from corresponding author on reasonable request not publicly available.

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Acknowledgements

The authors would like to acknowledge the Department of Biotechnology (DBT), Govt. of India, Ministry of Science and Technology for the financial support (project code BT/PR6489/AGII/106/880/2012) and CRP on Molecular Breeding, Indian Council of Agricultural Research (ICAR) (project code 12-143-H-TG-3121) to conduct research activities on Marker Assisted Breeding. The authors declare no conflict of interest.

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Authors and Affiliations

  1. Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India

    Tusar Kanti Behera, G. Boopalakrishnan, Gograj Singh Jat, Anilabha Das Munshi, Harshwardhan Choudhary, Amarnath Ravindran, Shilpi Kumari & Rajni Kumari

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  1. Tusar Kanti Behera
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  2. G. Boopalakrishnan
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  3. Gograj Singh Jat
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  4. Anilabha Das Munshi
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  5. Harshwardhan Choudhary
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  6. Amarnath Ravindran
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  7. Shilpi Kumari
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  8. Rajni Kumari
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Contributions

TKB conceived the experiments, writer, and analyst of results. TKB, ADM and HC supervised the project. GB, GSJ, AR, SK and RK collected data, provided technical support and management of crop. All authors reviewed and approved this submission.

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Correspondence to Tusar Kanti Behera.

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There is no conflicts of interest associated with this publication, and as corresponding author, I assure that all authors of this paper have fulfilled these authorship criteria and mentioned contribution of each author in text.

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Communicated by Younghoon Park.

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Behera, T.K., Boopalakrishnan, G., Jat, G.S. et al. Deriving stable tropical gynoecious inbred lines of slicing cucumber from American pickling cucumber using MABB. Hortic. Environ. Biotechnol. 63, 263–274 (2022). https://doi.org/10.1007/s13580-021-00392-5

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