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Establishment and characterization of a new intrahepatic cholangiocarcinoma cell line, ICC-X3

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Abstract

Intrahepatic cholangiocarcinoma (ICC) is an aggressive cancer of the biliary tract that is prone to recurrence and metastasis and is characterized by poor sensitivity to chemotherapy and overall prognosis. To address this challenge, the establishment of suitable preclinical models is critical. In this study, we successfully established a new ICC cell line, named ICC-X3, from the satellite lesions of one ICC patient. The cell line was characterized with respect to phenotypic, molecular, biomarker, functional and histological properties. STR confirmed that ICC-X3 was highly consistent with primary tumor tissue. ICC-X3 cells positively expressed CK7, CK19, E-cadherin, vimentin, and Ki67. ICC-X3 was all resistant to gemcitabine, paclitaxel, 5-FU, and oxaliplatin. The cell line was able to rapidly form xenograft tumors which were highly similar to the primary tumor. The missense mutation of TP53 exon was detected in ICC-X3 cells. ICC-X3 can be used as a good experimental model to study the progression, metastasis, and drug resistance of ICC.

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Data availability

The datasets used and/or analysed during the current study are available from the corresponding author upon reasonable request.

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Acknowledgements

We would like to thank Bullet Edits (http://www.bulletedits.cn) for English language editing of the manuscript.

Funding

This work was supported by grants from National Natural Science Foundation of China (Grant 82260555), the Gansu Provincial Science and Technology Plan (Grants 1606RJZA139, 21JR11RA096, 21JR1RA099, 21JR1RA113), Gansu Health Industry Project (Grant GSWSKY2020-21), Traditional Chinese Medicine Scientific Research Project of Gansu Province, China (Grant GZKP-2020–28),Talent Innovation and Entrepreneurship Project of Lanzhou (Grant 2020-RC-46), Lanzhou Science and Technology Plan Project (Grant 2022–3-45), Intra-Hospital Fund of the First Hospital of Lanzhou University (Grants ldyyyn-2014–01, ldyyyn2021-61 and ldyyyn2021-68).

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Author notes

  1. Hao Xu, Wei Luo, and Zhenjie Zhao contributed equally to this work and share first authorship.

Authors and Affiliations

  1. The Forth Department of General Surgery, the First Hospital of Lanzhou University, No. 1, Donggang West Road, LanZhou, 730000, Gansu, China

    Hao Xu, Wei Luo, Zhenjie Zhao, Changpeng Chai & Jinjing Hu

  2. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of the Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, LanZhou, 730000, China

    Xin Miao

  3. The Second Clinical Medical College, Lanzhou University, Lanzhou, 730000, China

    Huan Tang & Wence Zhou

  4. Department of General Surgery, the Second Hospital of Lanzhou University, Lanzhou, 730000, China

    Hui Zhang & Wence Zhou

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  1. Hao Xu
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Contributions

Conceptualization: HX, HZ and W-CZ; methodology: HX, WL, Z-JZ, XM and C-PC; software: HT, J-JH, HZ; validation: J-JH, HZ and Z-JZ; formal analysis: HX, WL; investigation: J-JH, HT, C-PC; resources: Z-JZ, WL and HZ; data curation: HX, XM and C-PC; writing–original draft preparation: HX; writing—review and editing: HX and W-CZ; visualization: HX, HT; supervision: HX, HZ; project administration: HX, and W-CZ; funding acquisition: HX, WL, Z-JZ, W-CZ, HZ, J-JH. All authors have read and agreed to the published version of the manuscript.

Corresponding authors

Correspondence to Hao Xu, Hui Zhang or Wence Zhou.

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The authors declare no competing interests.

Ethical approval

The study was conducted according to the guidelines of the Declaration of Helsinki, and approved by the Ethics Committee of the First Hospital of Lanzhou University (LDYYLL2022-345).

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We have obtained consents to publish this paper from all the participants of this study.

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Xu, H., Luo, W., Zhao, Z. et al. Establishment and characterization of a new intrahepatic cholangiocarcinoma cell line, ICC-X3. Human Cell 36, 854–865 (2023). https://doi.org/10.1007/s13577-023-00858-x

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