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Application of Multifactorial Experimental Design for Optimization of Streptokinase Production Using Streptococcus equisimilis SK-6

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Abstract

The study involves statistical experimental designs for optimizing culture conditions to enhance streptokinase synthesis by Streptococcus equisimilis SK-6. One-factor-at-a-time method of optimization demonstrated variables (glucose, tryptone and period of incubation) influenced streptokinase production. Further, optimization under shake flask fermentation was performed using central composite design of response surface methodology. The value of \(R^{2}\) (0.9403) indicated better association between the predicted and observed responses. Additionally, model F value of 17.50 and coefficient of variation (16.37) indicated the model to be significant (\(p>0.0001\)), leading to a highly reliable experimental design. Compared to the unoptimized conditions, a 3.2-fold increase in streptokinase production (0.269 U/ml) by S. equisimilis SK-6 was observed within 48 h in a medium containing tryptone (18 g/l) and glucose (10 g/l). This study is possibly the first attempt to optimize streptokinase synthesis from a wild strain of S. equisimilis by use of multifactorial experimental design, and hence it can be regarded as a model approach for enhancing the produce of streptokinase by industrial fermentation process.

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Acknowledgements

The authors are thankful to the management of Karpagam University for the encouraging support.

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Authors and Affiliations

  1. Department of Microbiology, Karpagam University, Coimbatore, Tamil Nadu, 641021, India

    Shilpi Bhardwaj & Muthusamy Palaniswamy

  2. Department of Microbiology, Center for Post Graduate Studies, Jain University, Bangalore, Karnataka, 560011, India

    Sourav Bhattacharya & Arijit Das

  3. Department of Microbial Biotechnology, Bharathiar University, Coimbatore, Tamil Nadu, 641046, India

    Jayaraman Angayarkanni

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  1. Shilpi Bhardwaj
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  2. Sourav Bhattacharya
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  3. Arijit Das
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  4. Muthusamy Palaniswamy
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  5. Jayaraman Angayarkanni
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Correspondence to Sourav Bhattacharya.

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Bhardwaj, S., Bhattacharya, S., Das, A. et al. Application of Multifactorial Experimental Design for Optimization of Streptokinase Production Using Streptococcus equisimilis SK-6. Arab J Sci Eng 42, 2273–2277 (2017). https://doi.org/10.1007/s13369-017-2475-5

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  • DOI: https://doi.org/10.1007/s13369-017-2475-5

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