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SSX2IP as a novel prognosis biomarker plays an important role in the development of breast cancer

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Abstract

Background

SSX2IP plays a critical role in tumorigenesis and its overexpression has been found in many types of cancer, such as an associated antigen in acute myeloid leukemia and breast cancer. However, the pathophysiological role of SSX2IP in BC has not been evaluated.

Objective

To investigate that SSX2IP gene plays an important role in the development of breast cancer, which can be used as a diagnostic marker for breast cancer.

Results

SSX2IP are detected in BC in this study, the result shown that SSX2IP mRNA and protein were upregulated in both human breast cancer samples and cell lines. With the depletion of SSX2IP, the results demonstrated the BT549 cell activity, proliferation, migratory and invasive, clone formation abilities were inhibited, and the cell apoptosis increased.

Conclusion

These data indicated that SSX2IP promotes tumor progression in BC, include cell activity, proliferation, migratory and invasive, clone formation abilities were inhibited, and the cell apoptosis increased. This work further suggest that SSX2IP could be used to be a potential therapeutic targets in BC.

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References

  • Asada M et al (2003) ADIP, a novel Afadin- and alpha-actinin-binding protein localized at cell-cell adherens junctions. J Biol Chem 278(6):4103–4111

    Article  CAS  PubMed  Google Scholar 

  • Chang et al (2020) Expression and prognostic utility of SSX2IP in patients with nasopharyngeal carcinoma. APMIS 128(4):287-297

  • Crew JC et al (1995) Fusion of SYT to two genes, SSX1 and SSX2, encoding proteins with homology to the Kruppel-associated box in human synovial sarcoma. EMBO J 14:2333–2340

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Denniss FA et al (2007) The leukaemia-associated antigen, SSX2IP, is expressed during mitosis on the surface of myeloid leukaemia cells. Br J Haematol 138:668–669

    Article  PubMed  Google Scholar 

  • Dronova TA et al (2021) Vascular endothelial growth factor receptor 2 (VEGFR2) contributes to tamoxifen resistance in estrogen-positive breast cancer patients. Mol Biol (Mosk) 55(1):118–125

    Article  CAS  PubMed  Google Scholar 

  • Fukumoto Y et al (2011) Role of scaffold protein afadin dilute domain-interacting protein (ADIP) in platelet-derived growth factor-induced cell movement by activating Rac protein through Vav2 protein. J Biol Chem 286:43537–43548

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Guinn BA et al (2008) SSX2IP expression in acute myeloid leukaemia: an association with mitotic spindle failure in t(8;21), and cell cycle in t(15;17) patients. Br J Haematol 140(2):250–251

    PubMed  Google Scholar 

  • Hu H et al (2021) Assessment of circulating HISLA as a poten-tial biomarker for breast cancer diagnosis and prognosis. Clin Exp Med 21(1):29–34

    Article  CAS  PubMed  Google Scholar 

  • Jakabova A et al (2021) Characterization of circulating tumor cells in early breast cancer patients receiving neoadjuvant chemotherapy. Ther Adv Med Oncol 13:17588359211028492

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Knuutila S et al (1988) DNA copy number amplifications in human neoplasms: review of comparative genomic hybridization studies. Am J Pathol 152(5):1107–23

    Google Scholar 

  • Kothari C et al (2021) TBC1D9: an important modulator of tumorigenesis in breast cancer. Cancers 13(14):3557

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lahiri T et al (2021) Mitochondrial STAT3 regulates antioxidant gene expression through complex I-derived NAD in triple negative breast cancer. Mol Oncol 15:1432–49

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lalaoui N et al (2020) Targeting triple-negative breast cancers with the Smac-mimetic birinapant. Cell Death Differ 27(10):2768–2780

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lee HH, Cho H (2020) Attenuated anti-tumor activity of NK-92 cells by invasive human breast carcinoma MDA-MB-231 cells. Mol Cell Toxicol 16:139–147

    Article  CAS  Google Scholar 

  • Lei JH et al (2021) Activation of FGFR2 signaling suppresses BRCA1 and drives triple-negative mammary tumorigenesis that is sensitive to immunotherapy. Adv Sci (Weinh) 8(21):e2100974

    Article  PubMed  Google Scholar 

  • Levesque LA, Roy S, Salazar N (2021) CXCR3 expression and genome-wide 3’ splice site selection in the TCGA breast cancer cohort. Life (Basel) 11(8):746

    CAS  PubMed  Google Scholar 

  • Li P et al (2013a) SSX2IP promotes metastasis and chemotherapeutic resistance of hepatocellular carcinoma. J Transl Med 11:52

    Article  PubMed  PubMed Central  Google Scholar 

  • Li P et al (2013b) Epigenetic silencing of miR-338-3p contributes to tumorigenicity in gastric cancer by targeting SSX2IP. PLoS ONE 8(6):e66782

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li ZX et al (2021) High BTBD7 expression positive is correlated with SLUG-predicted poor prognosis in hormone receptor-negative breast cancer. Ann Transl Med 9:1252

    Article  PubMed  PubMed Central  Google Scholar 

  • Lim FL et al (1998) A KRAB-related domain and a novel transcription repression domain in proteins encoded by SSX genes that are disrupted in human sarcomas. Oncogene 17:2013–2018

    Article  CAS  PubMed  Google Scholar 

  • Liu Y et al (2021) Highly heterogeneous-related genes of triple-negative breast cancer: potential diagnostic and prognostic biomarkers. BMC Cancer 21(1):644

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Loibl S et al (2021) Breast cancer. Lancet 397:1750–1769

    Article  CAS  PubMed  Google Scholar 

  • Myers KV, Amend SR, Pienta KJ (2019) Targeting Tyro3, Axl and MerTK (TAM receptors): implications for macrophages in the tumor microenvironment. Mol Cancer 18(1):94

    Article  PubMed  PubMed Central  Google Scholar 

  • Nowakowska MK et al (2021) Association of statin use with clinical outcomes in patients with triple-negative breast cancer. Cancer 127(22):4142–4150

    Article  CAS  PubMed  Google Scholar 

  • O’Reilly EA et al (2015) The fate of chemoresistance in triple negative breast cancer (TNBC). BBA Clin 3:257–275

    Article  PubMed  PubMed Central  Google Scholar 

  • Shen LW et al (2021) Cepharanthine sensitizes human triple negative breast cancer cells to chemotherapeutic agent epirubicin via inducing cofilin oxidation-mediated mitochondrial fission and apoptosis. Acta Pharmacol Sin 43(1):177–193

    Article  PubMed  PubMed Central  Google Scholar 

  • Sheng Y et al (2020) MicroRNA-4317 predicts the prognosis of breast cancer and inhibits tumor cell proliferation, migration, and invasion. Clin Exp Med 20(3):417–25

    Article  CAS  PubMed  Google Scholar 

  • Tsai YF et al (2021) Interleukin 17A promotes cell migration, enhances anoikis resistance, and creates a microenvironment suitable for triple negative breast cancer tumor metastasis. Cancer Immunol Immunother 70(8):2339–2351

    Article  CAS  PubMed  Google Scholar 

  • Wang X, Zheng Y, Wang Y (2021a) PEAK1 promotes invasion and metastasis and confers drug resistance in breast cancer. Clin Exp Med 9:23

    CAS  Google Scholar 

  • Wang Q et al (2021b) The expression and prognostic value of SUMO1-activating enzyme subunit 1 and its potential Mechanism in triple-negative breast cancer. Front Cell Dev Biol 9:729211

    Article  PubMed  PubMed Central  Google Scholar 

  • Wei JL et al (2021) GCH1 induces immunosuppression through metabolic reprogramming and IDO1 upregulation in triple-negative breast cancer. J Immunother Cancer 9(7):e002383

    Article  PubMed  PubMed Central  Google Scholar 

  • Wu SY et al (2021) MYC suppresses STING-dependent innate immunity by transcriptionally upregulating DNMT1 in triple-negative breast cancer. J Immunother Cancer 9(7):e002528

    Article  PubMed  PubMed Central  Google Scholar 

  • Xin L et al (2021) SIKs suppress tumor function and regulate drug resistance in breast cancer. Am J Cancer Res 11(7):3537–3557

    CAS  PubMed  PubMed Central  Google Scholar 

  • Yan Y et al (2021) CRYbetaB2 enhances tumorigenesis through upregulation of nucleolin in triple negative breast cancer. Oncogene 40(38):5752–5763

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yang L et al (2020) GPRC5A is a negative regulator of the pro-survival PI3K/Akt signaling pathway in triple-negative breast cancer. Front Oncol 10:624493

    Article  PubMed  Google Scholar 

  • Zhou YF et al (2021) Integrated analysis reveals prognostic value of HLA-I LOH in triple-negative breast cancer. J Immunother Cancer 9(10):e003371

    Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

This research was supported by Funds of the Key project of Anhui Provincial Department of Education (No. KJ2019A0341), the Science and Technology Plan Project of Suzhou (SLT201913), the Beijing Xisike Clinical Oncology Research Foundation (Y¬XD2019-227), and Horizontal Research Foundation of Soochow University (P142900221).

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Authors and Affiliations

  1. Department of Oncology, The First Affiliated Hospital of Soochow University, 899, Pinghai Road, Suzhou, 215006, Jiangsu, China

    Xianfu Liu & Min Tao

  2. Department of Oncology Surgery, The First Affiliated Hospital of Bengbu Medical College, 287 Changhuai Road, Bengbu, 233000, Anhui, China

    Xianfu Liu

  3. Department of Oncology, Dushu Lake Hospital Affiliated to Soochow University, 9, Chongwen Road, Suzhou, 215124, Jiangsu, China

    Min Tao

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  1. Xianfu Liu
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Contributions

MT and XFL designed the study, XFL provided technical support for experiments. XFL participated in the tissue sample selection and experiments. XFL performed experiments and analyzed data. XFL and MT wrote, reviewed and edited the manuscript. All authors have read and agreed to the published version of the manuscript.

Corresponding author

Correspondence to Min Tao.

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Conflict of interest

Prof. Tao declares that she has no conflict of interest. Mr. Liu declares that he has no conflict of interest.

Ethical approval

All the experimental procedures were performed in accordance with the ethical standards as recommended by the Human Ethics Committee and the Research Ethics Committee of the First Affiliated Hospital of Bengbu Medical College. Patients were informed that the resected specimens were stored by the hospital and potentially used for scientific research and publication of identifying information/images (when applicable), and that their privacy would be maintained. All patients provided informed consent prior to undergoing screening procedures.

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Liu, X., Tao, M. SSX2IP as a novel prognosis biomarker plays an important role in the development of breast cancer. Mol. Cell. Toxicol. 19, 463–472 (2023). https://doi.org/10.1007/s13273-022-00273-7

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  • DOI: https://doi.org/10.1007/s13273-022-00273-7

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