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Integrated analysis of miRNA and mRNA expression profiles in diabetic mouse kidney treated to Korean Red Ginseng

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Abstract

Background

Diabetes mellitus (DM) and its complications, including diabetic nephropathy, are rapidly increasing public health concerns. Korean Red Ginseng (KRG) is a popular herbal medicine that has many biological effects. The current literature demonstrates that KRG induced changes to the transcriptome in diabetic nephropathy mouse model are limited. Thus, we designed experiments to confirm whether changes of genes and miRNAs expression patterns in the renal cells of mice with DM, and then whether these changes are reversed by KRG.

Objective

This study aims to whether miRNAs and mRNAs expression and their relationships were contributed in the renal tissues of mice with streptozotocin (STZ)-induced diabetes.

Results

We determined that the expression levels of 46 miRNAs and 827 mRNAs expression were changed in KRG-treated DM renal cells compared to DM renal cells. In addition, we found that mRNAs expression shows anti-correlation with miRNAs expression and further validated their biological processes and signaling pathways by Gene Ontology (GO).

Conclusion

Our data suggest that KRG may regulate miRNA and gene expression in a way that relieves diabetic nephropathy in mice.

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Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF-2020R1A2C1011704) and Chung-Ang University Research Scholarship Grants 2020 to C.W. Kang.

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Authors and Affiliations

  1. Department of Microbiology, School of Medicine, Kyung Hee University, #26 Kyungheedae-gil, Dongdaemun-gu, Seoul, 02447, Korea

    Hye Rim Park, Seung Eun Lee & Yong Seek Park

  2. Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, Korea

    Yoojung Yi, Sangji Moon & Hyunkyung Yoon

  3. Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Chung-Ang University, Seoul, Korea

    Chae Won Kang & Jaetaek Kim

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  1. Hye Rim Park
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Contributions

Conceptualization, HRP, JK and YSP; Methodology, YY, SM, HY, CWK, SEL; validation, HRP; formal analysis, HRP; investigation, HRP and SEL; resources, HRP; writing-original draft preparation, HRP; writing-review and editing, YSP; visualization, HRP; supervision, YSP; project administration, YSP; funding acquisition, YSP. All authors have read and agreed to the published version of the manuscript.

Corresponding author

Correspondence to Yong Seek Park.

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Conflict of interest

Hye Rim Park declares that he/she has no conflict of interest. Seung Eun Lee declares that he/she has no conflict of interest. Yoojung Yi declares that he/she has no conflict of interest. Sangji Moon declares that he/she has no conflict of interest. Hyunkyung Yoon declares that he/she has no conflict of interest. Chae Won Kang declares that he/she has no conflict of interest. Jaetaek Kim declares that he/she has no conflict of interest. Yong Seek Park declares that he/she has no conflict of interest.

Human and animal rights

Ethical and experimental protocol approvals were obtained from the Institutional Animal Care and Use Committee of Kyung Hee University (Approval number: KHUASP (SE)-17–008).

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Park, H.R., Lee, S.E., Yi, Y. et al. Integrated analysis of miRNA and mRNA expression profiles in diabetic mouse kidney treated to Korean Red Ginseng. Mol. Cell. Toxicol. 18, 637–646 (2022). https://doi.org/10.1007/s13273-022-00272-8

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