Skip to main content
SpringerLink
Log in

Integrated miRNA and mRNA expression profiling in response to eriodictyol in human endothelial cells

  • Original Article
  • Published:
BioChip Journal Aims and scope Submit manuscript

Abstract

Eriodictyol, a flavonoid commonly found in citrus fruits, shows neurotrophic, antioxidant, anti- inflammatory, and anti-cancer effects under diverse pathophysiological conditions such as vascular diseases. In this study, we evaluated whether eriodictyol modulates miRNA and mRNA expression. Using microarray analysis, we examined miRNA and mRNA expression in human endothelial cells treated with 10 μM of eriodictyol for 24 h. Using numerous bioinformatic systems, we evaluated the signatures of the potential biological processes and signaling pathways. Our results provide insight into the underlying molecular mechanisms of action of eriodictyol and suggest that eriodictyol can be used for therapeutic intervention in vascular disease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bartel, D.P. MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell 116, 281–297 (2004).

    Article  CAS  Google Scholar 

  2. Zeng, Y. Principles of micro-RNA production and mat-uration. Oncogene 25, 6156–6162 (2006).

    Article  CAS  Google Scholar 

  3. Hayashita, Y. et al. A polycistronic microRNA cluster, miR-17-92, is overexpressed in human lung cancers and enhances cell proliferation. Cancer Res. 65, 9628–9632 (2005).

    Article  CAS  Google Scholar 

  4. Jovanovic, M. & Hengartner, M.O. miRNAs and apoptosis: RNAs to die for. Oncogene 25, 6176–6187 (2006).

    Article  CAS  Google Scholar 

  5. Schickel, R., Boyerinas, B., Park, S.M. & Peter, M.E. MicroRNAs: key players in the immune system, differentiation, tumorigenesis and cell death. Oncogene 27, 5959–5974 (2008).

    Article  CAS  Google Scholar 

  6. Kim, S.J. et al. Epigenetic Regulation of miR-22 in a BPA-exposed Human Hepatoma Cell. BioChip J. 9, 76–84 (2015).

    Article  CAS  Google Scholar 

  7. Lee, W. et al. Expression profiling of microRNAs in lipopolysaccharide-induced acute lung injury after hypothermia treatment. Mol. Cell. Toxicol. 12, 243–253 (2016).

    Article  CAS  Google Scholar 

  8. Cha, H.J. et al. MicroRNA expression profiling of p-phenylenediamine treatment in human keratinocyte cell line. Mol. Cell. Toxicol. 11, 19–28 (2015).

    Article  CAS  Google Scholar 

  9. Joshi, S.R., McLendon, J.M., Comer, B.S. & Gerthoffer, W.T. MicroRNAs-control of essential genes: Implications for pulmonary vascular disease. Pulm. Circ. 1, 357–364 (2011).

    Article  CAS  Google Scholar 

  10. Lee, S.E. et al. MicroRNA and gene expression analysis of melatonin-exposed human breast cancer cell lines indicating involvement of the anticancer effect. J. Pineal Res. 51, 345–352 (2011).

    Article  CAS  Google Scholar 

  11. Sayed, A.S.M., Xia, K., Salma, U., Yang, T.L. & Peng, J. Diagnosis, Prognosis and Therapeutic Role of Circulating miRNAs in Cardiovascular Diseases. HEART LUNG CIRC 23, 503–510 (2014).

    Article  Google Scholar 

  12. Lee, S.E. et al. Identification and Characterization of MicroRNAs in Acrolein-Stimulated Endothelial Cells: Implications for Vascular Disease. BioChip J. 9, 144–155 (2015).

    Article  CAS  Google Scholar 

  13. Katoh, M. Therapeutics targeting angiogenesis: genetics and epigenetics, extracellular miRNAs and signaling networks (Review). Int. J. Mol. Med. 32, 763–767 (2013).

    Article  CAS  Google Scholar 

  14. Choi, H.N., Kang, M.J. & Kim, J.I. Antioxidant effect of myricetin in animal model of type 2 diabetes. FASEB J. 27 (2013).

    Google Scholar 

  15. Onozuka, H. et al. Nobiletin, a citrus flavonoid, improves memory impairment and Abeta pathology in a transgenic mouse model of Alzheimer’s disease. J. Pharmacol. Exp. Ther. 326, 739–744 (2008).

    Article  CAS  Google Scholar 

  16. Ismaili, H. et al. Topical anti-inflammatory activity of extracts and compounds from Thymus broussonettii. J. Pharm. Pharmacol. 54, 1137–1140 (2002).

    Article  CAS  Google Scholar 

  17. Minato, K. et al. Lemon flavonoid, eriocitrin, suppresses exercise-induced oxidative damage in rat liver. Life Sci. 72, 1609–1616 (2003).

    Article  CAS  Google Scholar 

  18. Lee, E. et al. Binding model for eriodictyol to Jun-N terminal kinase and its anti-inflammatory signaling pathway. BMB Reports 46, 594–599 (2013).

    Article  CAS  Google Scholar 

  19. Zhang, X. et al. Anti-inflammatory activity of flavonoids from Populus davidiana. Arch. Pharm. Res. 29, 1102–1108 (2006).

    Article  CAS  Google Scholar 

  20. Narvaez-Mastache, J.M., Novillo, F. & Delgado, G. Antioxidant aryl-prenylcoumarin, flavan-3-ols and flavonoids from Eysenhardtia subcoriacea. Phytochemistry 69, 451–456 (2008).

    Article  CAS  Google Scholar 

  21. Lee, S.E. et al. Eriodictyol Protects Endothelial Cells against Oxidative Stress-Induced Cell Death through Modulating ERK/Nrf2/ARE-Dependent Heme Oxygenase-1 Expression. Int. J. Mol. Sci. 16, 14526–14539 (2015).

    Article  CAS  Google Scholar 

  22. Hanneken, A., Lin, F.F., Johnson, J. & Maher, P. Flavonoids protect human retinal pigment epithelial cells from oxidative-stress-induced death. Invest. Ophthalmol. Vis. Sci. 47, 3164–3177 (2006).

    Article  Google Scholar 

  23. Johnson, J., Maher, P. & Hanneken, A. The flavonoid, eriodictyol, induces long-term protection in ARPE-19 cells through its effects on Nrf2 activation and phase 2 gene expression. Invest. Ophthalmol. Vis. Sci. 50, 2398–2406 (2009).

    Article  Google Scholar 

  24. Bucolo, C., Leggio, G.M., Drago, F. & Salomone, S. Eriodictyol prevents early retinal and plasma abnormalities in streptozotocin-induced diabetic rats. Biochem. Pharmacol. 84, 88–92 (2012).

    Article  CAS  Google Scholar 

  25. Lee, J.K. Anti-inflammatory effects of eriodictyol in lipopolysaccharide-stimulated raw 264.7 murine macrophages. Arch. Pharm. Res. 34, 671–679 (2011).

    Article  CAS  Google Scholar 

  26. Lou, H., Jing, X., Ren, D., Wei, X. & Zhang, X. Eriodictyol protects against H(2)O(2)-induced neuron-like PC12 cell death through activation of Nrf2/ARE signaling pathway. Neurochem. Int. 61, 251–257 (2012).

    Article  CAS  Google Scholar 

  27. Lee, S.E. et al. Induction of thioredoxin reductase 1 by crotonaldehyde as an adaptive mechanism in human endothelial cells. Mol. Cell. Toxicol. 11, 433–439 (2015).

    Article  CAS  Google Scholar 

  28. Sumpio, B.E., Riley, J.T. & Dardik, A. Cells in focus: endothelial cell. Int. J. Biochem. Cell Biol. 34, 1508–1512 (2002).

    Article  CAS  Google Scholar 

  29. Dauchet, L. et al. Frequency of fruit and vegetable consumption and coronary heart disease in France and Northern Ireland: the PRIME study. Br. J. Nutr. 92, 963–972 (2004).

    Article  CAS  Google Scholar 

  30. Chanet, A. et al. Naringin, the major grapefruit flavonoid, specifically affects atherosclerosis development in diet-induced hypercholesterolemia in mice. J. Nutr. Biochem. 23, 469–477 (2012).

    Article  CAS  Google Scholar 

  31. Hirose, E. et al. Involvement of Heme Oxygenase-1 in Kaempferol-Induced Anti-Allergic Actions in RBL-2H3 Cells. Inflammation 32, 99–108 (2009).

    Article  CAS  Google Scholar 

  32. Lin, H.Y., Juan, S.H., Shen, S.C., Hsu, F.L. & Chen, Y.C. Inhibition of lipopolysaccharide-induced nitric oxide production by flavonoids in RAW264.7 macrophages involves heme oxygenase-1. Biochem. Pharmacol. 66, 1821–1832 (2003).

    Article  CAS  Google Scholar 

  33. Ferreira, E.D. et al. Neuroinflammatory response to experimental stroke is inhibited by eriodictyol. Behav. Brain Res. 312, 321–332 (2016).

    Article  CAS  Google Scholar 

  34. Jing, X. et al. Eriodictyol-7-O-glucoside activates Nrf2 and protects against cerebral ischemic injury. Toxicol. Appl. Pharmacol. 273, 672–679 (2013).

    Article  CAS  Google Scholar 

  35. Saunders, M.A. & Lim, L.P. (micro)genomic medicine microRNAs as therapeutics and biomarkers. RNA Biol. 6, 324–328 (2009).

    Article  CAS  Google Scholar 

  36. Son, G.W. et al. Analysis of miRNA expression profiling in melatonin-exposured endothelial cells. Mol. Cell. Toxicol. 12, 73–81 (2016).

    Article  CAS  Google Scholar 

  37. Barber, G.N. et al. The 58-Kilodalton Inhibitor of the Interferon-Induced Double-Stranded Rna-Activated Protein-Kinase Is a Tetratricopeptide Repeat Protein with Oncogenic Properties. Proc. Natl. Acad. Sci. U. S. A. 91, 4278–4282 (1994).

    Article  CAS  Google Scholar 

  38. Petrova, K., Oyadomari, S., Hendershot, L.M. & Ron, D. Regulated association of misfolded endoplasmic reticulum lumenal proteins with P58/DNAJc3. EMBO J. 27, 2862–2872 (2008).

    CAS  Google Scholar 

  39. Tao, J.H. & Sha, B.D. Structural Insight into the Protective Role of P58(Ipk) during Unfolded Protein Response. Methods in Enzymology: Unfolded Protein Response and Cellular Stress, Vol 490, Pt B 490, 259–270 (2011).

    Google Scholar 

  40. Rutkowski, D.T. et al. The role of p58IPK in protecting the stressed endoplasmic reticulum. Mol. Biol. Cell 18, 3681–3691 (2007).

    Article  CAS  Google Scholar 

  41. Yan, W. et al. Control of PERK eIF2alpha kinase activity by the endoplasmic reticulum stress-induced molecular chaperone P58IPK. Proc. Natl. Acad. Sci. U. S. A. 99, 15920–15925 (2002).

    Article  CAS  Google Scholar 

  42. Yan, W., Suominen, J. & Toppari, J. Stem cell factor protects germ cells from apoptosis in vitro. J. Cell. Sci. 113, 161–168 (2000).

    CAS  Google Scholar 

  43. Ge, D. et al. Phosphorylation and nuclear translocation of integrin beta4 induced by a chemical small molecule contribute to apoptosis in vascular endothelial cells. Apoptosis 18, 1120–1131 (2013).

    Article  CAS  Google Scholar 

  44. Kim, S.Y. et al. Identification of potential biomarkers for xylene exposure by microarray analyses of gene expression and methylation. Mol. Cell. Toxicol. 12, 15–20 (2016).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, School of Medicine, Kyung Hee University, Seoul, 02447, Republic of Korea

    Seung Eun Lee, Jeong-Je Cho, Hyun-Jong Ahn, Cheung-Seog Park & Yong Seek Park

  2. Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, 02447, Republic of Korea

    Hye Rim Park & Hong Duck Yun

Authors
  1. Seung Eun Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hye Rim Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hong Duck Yun
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jeong-Je Cho
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hyun-Jong Ahn
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Cheung-Seog Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yong Seek Park
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yong Seek Park.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lee, S.E., Park, H.R., Yun, H.D. et al. Integrated miRNA and mRNA expression profiling in response to eriodictyol in human endothelial cells. BioChip J 11, 188–195 (2017). https://doi.org/10.1007/s13206-017-1303-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13206-017-1303-z

Keywords

Search

Navigation