Abstract
In this research, the antimicrobial substance anti-JFL15 was partially purified using a simple two-step extraction process from the cell-free supernatants of Bacillus siamensis JFL15. Anti-JFL15 exhibited a strong antibacterial activity against various multidrug-resistant aquatic bacterial pathogens, including Escherichia coli, Edwardsiella tarda, Pseudomonas aeruginosa, Aeromonas hydrophila, and Vibrio. Liquid chromatography–mass spectrometry revealed that anti-JFL15 contained eight cyclic lipopeptides belonging to two families: bacillomycin F (m/z 1056.56–1084.59) and surfactin (m/z 1007.65–1049.70) analogs. PCR analysis showed the presence of genes (i.e., sfp gene, surfactin synthetase D, fengycin synthetase B, iturin synthetase A, iturin synthetase C and bacillomycin synthetase D) involved in the biosynthesis of cyclic lipopeptides. This study is the first to identify cyclic lipopeptides from B. siamensis and use them to suppress the growth of various multidrug-resistant aquatic bacterial pathogens. Results indicated that B. siamensis JFL15 is a promising biocontrol agent for the effective and environmentally friendly control of various multidrug-resistant aquatic bacterial pathogens.
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Acknowledgements
We would like to thank the Projects of Science and Technology of Guangdong Province (Grant numbers 2015A020209121, 2015A030313425 and 2015A030310225), and the Project of Science and Technology of Guangzhou City (Grant number 201607010197) supported this work. We are grateful to Professor Erxun Zhou for his kind providing of indicator fungi strains M. grisea and R. solani, and Professor Zaiguang Fang of Hainan University Institute of Marine for his kind providing of indicator bacteria.
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Xu, BH., Ye, ZW., Zheng, QW. et al. Isolation and characterization of cyclic lipopeptides with broad-spectrum antimicrobial activity from Bacillus siamensis JFL15. 3 Biotech 8, 444 (2018). https://doi.org/10.1007/s13205-018-1443-4
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DOI: https://doi.org/10.1007/s13205-018-1443-4