Skip to main content
SpringerLink
Log in

Genetic variability in selected date palm (Phoenix dactylifera L.) cultivars of United Arab Emirates using ISSR and DAMD markers

  • Original Article
  • Published:
3 Biotech Aims and scope Submit manuscript

Abstract

Nine (9) different date palm (Phoenix dactylifera L.) cultivars from UAE, which differ in their flower timings were selected to determine the polymorphism and genetic relationship between these cultivars. Hereditary differences and interrelationships were assessed utilizing inter-simple sequence repeat (ISSR) and directed amplification of minisatellite DNA region (DAMD) primers. Analysis on eight DAMD and five ISSR markers produced total of 113 amplicon including 99 polymorphic and 14 monomorphic alleles with a polymorphic percentage of 85.45. The average polymorphic information content for the two-marker system was almost similar (DAMD, 0.445 and ISSR, 0.459). UPGMA based clustering of DAMD and ISSR revealed that mid-season cultivars, Mkh (Khlas) and MB (Barhee) grouped together to form a subcluster in both the marker systems. The genetic similarity analysis followed by clustering of the cumulative data from the DAMD and ISSR resulted in two major clusters with two early-season cultivars (ENg and Ekn), two mid-season cultivars (MKh and MB) and one late-season cultivar (Lkhs) in cluster 1, cluster 2 includes two late-season cultivars, one early-season cultivar and one mid-season cultivar. The cluster analysis of both DAMD and ISSR marker revealed that, the patterns of variation between some of the tested cultivars were similar in both DNA marker systems. Hence, the present study signifies the applicability of DAMD and ISSR marker system in detecting genetic diversity of date palm cultivars flowering at different seasons. This may facilitate the conservation and improvement of date palm cultivars in the future.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Abbreviations

DAMD:

Directly amplified minisatellite DNA

ISSR:

Inter-simple sequence repeat

PCR:

Polymerase chain reaction

Date palm genotypes:

Nagal (ENg), Shaham (ESh), Khanezi (EKn) Barhee (MB), Khlas (MKh), Nabthasaif (MNb) Lulu (LL), Khasab (LKhs), Fardh (LF)

References

  • Arif A, Bakir MA, Khan HA, Ahamed A, Ahmad HA, Farhan A, Ali A, Homaidan MA, Sadoon AH, Bahkali M, Shobrak A (2010) simple method for DNA extraction from mature date palm leaves: impact of sand grinding and composition of lysis buffer. Int J Mol Sci 11(9):3149–3157

    Article  CAS  Google Scholar 

  • Babeli PJ, Zhou Z, Somer DJ, Gustafson JP (1997) PCR primed with minisatellite core sequences yields DNA fingerprinting probes in wheat. Theor Appl Genet 95:276–283

    Article  Google Scholar 

  • Bhattacharya E, Ranade SA (2001) Molecular distinction amongst varieties of mulberry using RAPD and DAMD profiles. BMC Plant Biol 1(3):1–8

    Google Scholar 

  • Bhattacharya E, Dandin SB, Ranade SA (2005) Single primer amplification reaction methods reveal exotic and indigenous mulberry varieties are similarly diverse. J Biosci 30:669–675

    Article  CAS  Google Scholar 

  • Bornet BC, Muller FP, Branchard M (2002) Highly informative nature of inter simple sequence repeat (ISSR) sequences amplified using tri- and tetra-nucleotide primers from DNA of cauliflower (Brassica oleracea var. ‘botrytis’ L.). Genome 45:890–896

    Article  CAS  Google Scholar 

  • Brantestam AK, Bothmer RV, Dayteg C, Rashal I, Tuvesson S, Weibull J (2004) Inter simple sequence repeat analysis of genetic diversity and relationships in cultivated barley of Nordic and Baltic origin. Hereditas 141(2):186–187

    Article  Google Scholar 

  • Chao CT, Krueger RR (2007) The date palm (Phoenix dactylifera L.): overview of biology, uses, and cultivation. HortScience 42:1046–1311

    Google Scholar 

  • Cheruth AJ, Kurup SS, Subramaniam S (2015) Variations in hormones and antioxidant status in relation to flowering in early, mid, and late varieties of date palm (Phoenix dactylifera) of United Arab Emirates. The Sci World J 2015:846104. https://doi.org/10.1155/2015/846104

  • El Hadrami A, Al-Khayri JM (2012) Socioeconomic and traditional importance of date palm. Emir J Food Agric 24:371–385

    Google Scholar 

  • Elmeer K, Sarwath H, Malek J (2011) New microsatellite markers for assessment of genetic diversity in date palm (Phoenix dactylifera L.). 3. Biotech 1(2):91–97

    Google Scholar 

  • Elsadig E, Aljuburi H, Elamin A, Gafar M (2017) Impact of organic manure and combination of N P K S, on yield, fruit quality and fruit mineral content of Khenazi date palm (Phoenix dactylifera L.) cultivar. J Sci Agric 1:335–346

    Google Scholar 

  • Gupta M, Chyi YS, Severson JR, Owen JL (1994) Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple-sequence repeats. Theor Appl Genet 89:998–1006

    CAS  Google Scholar 

  • Hamwieh A, Upada SM, Sarker A, Jung C, Baum M (2009) Development of new microsatellite markers and their application in the analysis of genetic diversity in lentil. Breed Sci 59:77–86

    Article  CAS  Google Scholar 

  • Heath DD, Iwama GK, Devlin RH (1993) PCR primed with VNTR core sequences yield species specific patterns and hypervariable probes. Nucleic Acids Res 21:5782–5785

    Article  CAS  Google Scholar 

  • Hu JB, Li JW, Wang LJ, Liu LJ, Si SW (2011) Utilization of a set of high-polymorphism DAMD markers for genetic analysis of a cucumber germplasm collection. Acta Physiol Plant 33:227–231

    Article  Google Scholar 

  • Karim K, Chokri B, Amel S, Wafa H, Richid H, Nouredine D (2010) Genetic diversity of Tunisian date palm germplasm using ISSR markers. Int J Bot 6:182–186

    Article  CAS  Google Scholar 

  • Khanam S, Sham A, Bennetgen JL, Mohammed AMA (2012) Analysis of molecular marker-based characterization and genetic variation in date palm (Phoenix dactylifera L.) Aust J. Crop Sci 6:1236–1244

    Google Scholar 

  • Kurup SS, Hedar YS, Al Dhaheri MA, El-Heawiety AY, Aly MAM, Alhadrami G (2009) Morpho-physiological evaluation and RAPD markers-assisted characterization of date palm (Phoenix dactylifera L.) varieties for salinity tolerance. J Food Agric Env 7(3&4):503–507

    CAS  Google Scholar 

  • Mansouri A, Embarek G, Kokkalou E, Kefalas P (2005) Phenolic profile and antioxidant activity of the Algerian ripe date palm fruit (Phoenix dactylifera). Food Chem 89:411–420

    Article  CAS  Google Scholar 

  • Mantel NA (1967) The detection of disease clustering and a generalized regression approach. Cancer Res 27(2):209–220

    CAS  Google Scholar 

  • Narzary D, Mahar KS, Rana TS, Ranade SA (2009) Analysis of genetic diversity among wild pomegranates in Western Himalayas, using PCR methods. Sci Hortic 121:237–242

    Article  CAS  Google Scholar 

  • Ranade SA, Rana TS, Narzary D (2009) SPAR profile and genetic diversity amongst pomegranate (Punica granatum L.) genotypes. Physiol Mol Biol Plants 15:61–70

    Article  CAS  Google Scholar 

  • Rohlf FJ (1998) NTSYS-PC: numerical taxonomy and multivariate analysis system. Version 2.02, Exeter software. Setauket, New York

  • Roldan-Ruiz I, Dendauw J, Vanbockstaele E, Depicker A, De Loose M (2000) AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Mol Breed 6:125–134

    Article  CAS  Google Scholar 

  • Rout GR, Senapati SK, Aparajita S, Palai SK (2009) Studies on genetic identification and genetic fidelity of cultivars banana using ISSR marker. Plant Omics J 2:250–258

    CAS  Google Scholar 

  • Singh N, Bajpai R, Mahar KS, Tiwari V, Upreti DK, Rana TS (2014) ISSR and DAMD markers revealed high genetic variability within Flavoparmelia caperata in Western Himalaya (India). Physiol Mol Biol Plants 20(4):501–508

    Article  CAS  Google Scholar 

  • Williams JGK, Rubelik AR, Livak KJ, Rafalski A, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

    Article  CAS  Google Scholar 

  • Zehdi S, Trifi M, Ould MSA, Rhouma A, Marrakchi M (2004) Molecular characterization of Tunisian date palm germplasm using ISSR markers. J Genet Breed 56:77–83

    Google Scholar 

  • Zhou Z, Bebeli PJ, Somers DJ, Gustafson JP (1997) Direct amplification of minisatellite-region DNA with VNTR core sequences in the genus Oryza. Theor Appl Genet 95:942–949

    Article  CAS  Google Scholar 

  • Zietkiewicz E, Rafalski A, Labuda D (1994) Genome fingerprint by simple sequence repeat (SSR)—anchored polymerase chain reaction amplification. Genomics 20:176–183

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors would like to thank the Department of Aridland Agriculture, College of food and Agriculture, United Arab Emirates University, Al Ain, United Arab Emirates, for the facilities. The farm supervisor and labourers of Al-Foah experimental station are gratefully acknowledged. This research is supported by the UAEU Program for Advanced Research-UPAR (Grant: UPAR # 31F045), received by the principal investigator Dr. Abdul J. Cheruth.

Author information

Authors and Affiliations

  1. Department of Aridland Agriculture, College of Food and Agriculture, United Arab Emirates University, P.O. Box 15551, Al-Ain, United Arab Emirates

    Fayas T. Purayil, Gabriel A. Robert, Shyam S. Kurup & Abdul Jaleel Cheruth

  2. Department of Botany, A.V.C College (Autonomous), Mannampandal, Tamil Nadu, India

    Gabriel A. Robert

  3. School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu, India

    Kodiveri M. Gothandam

  4. School of Biological Sciences, Universiti Sains, Malaysia (USM), Georgetown, 11800, Georgetown, Penang, Malaysia

    Sreeramanan Subramaniam

Authors
  1. Fayas T. Purayil
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gabriel A. Robert
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kodiveri M. Gothandam
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shyam S. Kurup
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sreeramanan Subramaniam
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Abdul Jaleel Cheruth
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Abdul Jaleel Cheruth.

Ethics declarations

Conflict of Interest

The authors declared that there is no conflict of interest.

Additional information

Sreeramanan Subramaniam: Temporarily corresponding author upon acceptance.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Purayil, F.T., Robert, G.A., Gothandam, K.M. et al. Genetic variability in selected date palm (Phoenix dactylifera L.) cultivars of United Arab Emirates using ISSR and DAMD markers. 3 Biotech 8, 109 (2018). https://doi.org/10.1007/s13205-018-1108-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s13205-018-1108-3

Keywords

Search

Navigation