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A halotolerant laccase from Chaetomium strain isolated from desert soil and its ability for dye decolourization

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Abstract

A novel fungal laccase produced by the ascomycete Chaetomium sp. isolated from arid soil was purified and characterized and its ability to remove dyes was determined. Extracellular laccase was purified 15-fold from the crude culture to homogeneity with an overall yield of 50% using ultrafiltration and anion-exchange chromatography. The purified enzyme was found to be a monomeric protein with a molecular mass of 68 kDa, estimated by SDS-PAGE, and with an isoelectric point of 5.5. The optimal temperature and pH value for laccase activity toward 2,6-DMP were 60 °C and 3.0, respectively. It was stable at temperatures below 50 °C and at alkaline conditions. Kinetic study showed that this laccase showed higher affinity on ABTS than on 2,6-DMP. Its activity was enhanced by the presence of several metal ions such as Mg2+, Ca2+ and Zn2+, while it was strongly inhibited by Fe2+, Ag+ and Hg2+. The novel laccase also showed high, remarkable sodium chloride tolerance. Its ability to decolorize different dyes, with or without HBT (1-hydroxy-benzotriazole), as redox mediator, suggests that this protein may be useful for different industrial applications and/or bioremediation processes.

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Acknowledgements

We thank Marίa Jesùs Martίnez and Jorge Barriuso for their help in carrying out this work.

Author information

Author notes

  1. Tahar Mechichi

    Present address: Laboratory of Enzyme Engineering and Microbiology, National School of Engineers of Sfax, University of Sfax, Route de Soukra Km 4.5, BP 1173, 3038, Sfax, Tunisia

Authors and Affiliations

  1. Laboratory of Enzyme Engineering and Microbiology, National School of Engineers of Sfax, University of Sfax, Route de Soukra Km 4.5, BP 1173, 3038, Sfax, Tunisia

    Rim Mtibaà, Bouthaina Ghariani, Ibtihel Louati & Moncef Nasri

  2. Department of Environmental Biology, Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain

    Laura de Eugenio

  3. Laboratory of Soil Biology, University of Neuchâtel, Rue Emile Argand 11, 2009, Neuchâtel, Switzerland

    Lasaad Belbahri

Authors
  1. Rim Mtibaà
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  2. Laura de Eugenio
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  3. Bouthaina Ghariani
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  4. Ibtihel Louati
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  5. Lasaad Belbahri
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  6. Moncef Nasri
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  7. Tahar Mechichi
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Corresponding author

Correspondence to Tahar Mechichi.

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Mtibaà, R., de Eugenio, L., Ghariani, B. et al. A halotolerant laccase from Chaetomium strain isolated from desert soil and its ability for dye decolourization. 3 Biotech 7, 329 (2017). https://doi.org/10.1007/s13205-017-0973-5

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  • DOI: https://doi.org/10.1007/s13205-017-0973-5

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