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Changes of visceral properties and digestive enzymes in the herbivorous marine teleost Siganus canaliculatus fed on different diets

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Abstract

The rabbitfish Siganus canaliculatus is one of the few cultured herbivorous marine teleosts. To better understand the digestive physiology of this fish and provide data for designing formulated feed using macroalgae as an ingredient, the changes of visceral properties and digestive enzyme activities were investigated after the juveniles were fed on different types of food including raw fish (RF), formulated diet (FD) or macroalgae Enteromorpha prolifra (EP) and Gracilaria lemaneiformis (GL) for eight weeks. The results showed that the hepatosomatic and viscerosomatic indices in the RF and FD groups, as well as the relative intestine length (RIL) in the EP and GL groups, were significantly higher than those in other groups. Additionally, differences in the histological structure of the liver and anterior intestine were also observed among different dietary groups. The hepatic nuclei were displaced to the periphery by lipid inclusions in fish fed RF. The highest levels of mucosal folds were found in the anterior intestines of fish fed macroalgae. Digestive enzyme activity profiles showed obvious fluctuations in the first three weeks, and then leveled off in the following weeks. The levels of protease, lipase and α-amylase in the alimentary tract showed changes related to the levels of dietary protein, lipid and carbohydrate, respectively. Although macroalgae significantly inhibited the activity of protease in the stomach, it increased RIL and the number of mucosal folds in the anterior intestine so as to compensate for the influences on protease activities in the stomach. This study suggests that the digestive tract of rabbitfish can well adapt to different diets, and needs about three weeks to physiologically acclimatize to the nutritional status, thus implying that rabbitfish are somewhat omnivorous.

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China

    Dizhi Xie & Yuanyou Li

  2. Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, China

    Shude Xu, Qingyang Wu, Fang Chen, Shuqi Wang & Cuihong You

  3. Guangdong VTR Bio-tech Co., Ltd, Zhuhai, 519060, China

    Shude Xu

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  1. Dizhi Xie
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Correspondence to Yuanyou Li.

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Foundation item: The Major International Joint Research Project from the National Natural Science Foundation of China (NSFC) under contract No. 31110103913; the NSFC Youth Project under contract No. 31602176; China Agriculture Research System under contract No. CARS-47.

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Xie, D., Xu, S., Wu, Q. et al. Changes of visceral properties and digestive enzymes in the herbivorous marine teleost Siganus canaliculatus fed on different diets. Acta Oceanol. Sin. 37, 85–93 (2018). https://doi.org/10.1007/s13131-018-1165-9

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