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First report on molecular characterization of Oestrus ovis in sheep from India

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Abstract

The objective of the present study is to characterize the dipteran larvae species infesting the sheep being maintained at SRRC, Mannavanur, by means of COI gene based PCR. During the last week of May 2021, post mortem examination of the skull of an Avikalin male sheep (20 months old) revealed the presence of larvae in its nasal sinuses. The larvae were washed in PBS (pH 7.2) and preserved in 70% alcohol. Total genomic DNA was isolated from the larvae using an initial step of grinding with liquid Nitrogen in a sterile mortar and pestle. Using the isolated genomic DNA from the larvae as a template, Cytochrome c oxidase subunit I (COI) gene based PCR was employed using the primers designed based on the COI gene of reference isolate of Oestrus ovis available in the GenBank. Full length COI gene (1534 bp) gene of Oestrus ovis in sheep from South India was targeted in the PCR experiment. The pTZ57R/T vector was used for the cloning of the PCR amplified fragment and the confirmed recombinant plasmid was subjected to sequencing experiments. In addition to morphological examination, based on COI gene based PCR, eventual sequencing experiments and BLAST analysis, it was confirmed that the larvae in the nasal sinuses of sheep from South India were Oestrus ovis. The South Indian isolate of Oestrus ovis is sharing 100% sequence identity both at nucleotide and amino acid levels with that of O. ovis from Spain. The North Indian isolate of O. ovis (from Jammu) exhibited 92% and 99% identity at respective nucleotide and amino acid levels with South Indian isolate. With other members of the subfamily Oestrinae, the share of per cent nucleotide and amino acid identities of South Indian O. ovis ranged from 85–86% to 95–96%, respectively. O. ovis from South India was grouped with the other members of Oestrinae from different geographical areas of the globe in the analysis of phylogenetic tree based on COI amino acid sequences. Based on the research findings, it is concluded that Oestrus ovis is the dipteran species infesting the sheep at Mannavanur, Tamil Nadu, India. To our knowledge, this is the first report on full length nucleotide sequences of COI gene of O. ovis in sheep from Indian subcontinent.

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Acknowledgements

The authors gratefully acknowledged the facilities and fund rendered by the Director, ICAR-Central Sheep and Wool Research Institute, Avikanagar, Rajasthan for carrying out the study. The authors are thankful to the Director, ICAR-Sugarcane Breeding Institute, Coimbatore, Tamil Nadu, India for the grant of the permission to avail the lab facilities. The authors are also thankful to the Dr. Mrs. Selvi A., Principal Scientist & Head, Biotechnology Section, Division of Crop Improvement, ICAR-Sugarcane Breeding Institute, Coimbatore – 641007, Tamil Nadu, who had given the moral support and supervision during the entire course of the work. The authors are also extending their heartfelt thanks to Dr. C. Appunu, Sr. Scientist, for sharing his lab facilities in carrying out the present study. Special thanks are due to Dr. A.S. Rajendiran, Officer Incharge, SRRC, Mannavanur, who had motivated me in carrying out biotechnological works. Further, the help rendered by Dr. S. Rajapandi, CTO, Sh. G. Murali, STO, Sh. K. Ganesan, Technical Assistant & contractual workers especially Sh. A. Kapil Dev, at SRRC, Mannavanur as well as Sh. M. Ram Vannish, Young Professional, ICAR-Sugarcane Breeding Institute, Coimbatore – 641007, Tamil Nadu, in the collection and processing of larvae from sheep, is also gratefully acknowledged. The financial support provided by Indian Council of Agricultural Research to carry out this study is gratefully acknowledged.

Funding

The present reserch work has been carried out under the institute fund coverage of ICAR-Central Sheep and Wool Research Institute, Avikanagar, Rajasthan, India.

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Author notes

  1. G. Nagarajan and K. Kanagarajadurai have been contributed equally.

Authors and Affiliations

  1. ICAR-Central Sheep and Wool Research Institute, Avikanagar, Rajasthan, 304501, India

    G. Nagarajan & S. M. K. Thirumaran

  2. Veterinary University Training and Diagnostic Centre, TANUVAS, Madurai, Tamil Nadu, 625005, India

    K. Kanagarajadurai

  3. Southern Regional Research Centre (ICAR-CSWRI), Mannavanur, Kodaikanal, Tamil Nadu, 624103, India

    K. Pachaiyappan, S. Jegaveera Pandian & P. Thirumurugan

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  1. G. Nagarajan
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Correspondence to G. Nagarajan.

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All animal experiments were carried out as per the approval from the Institute Animal Ethical Committee of SRRC, Mannavanur, Tamil Nadu, India.

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12639_2024_1666_MOESM1_ESM.ppt

Supplementary Fig. 1 Alignment of first 420 amino acid sequences (from 1 to 420 in the amino terminal) of COI gene of Oestrus ovis from Mannavanur with that of different dipteran flies from various geographical areas of the world. The upward arrows indicate the conserved amino acid positions. (PPT 1904 KB)

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Nagarajan, G., Kanagarajadurai, K., Pachaiyappan, K. et al. First report on molecular characterization of Oestrus ovis in sheep from India. J Parasit Dis (2024). https://doi.org/10.1007/s12639-024-01666-2

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