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Effects of the Probiotic Candidate E. faecalis-1, the Poulvac E. coli Vaccine, and their Combination on Growth Performance, Caecal Microbial Composition, Immune Response, and Protection against E. coli O78 Challenge in Broiler Chickens

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Abstract

The present study was performed on 180-day-old commercial Cobb chicks to assess the effects of the probiotic candidate Enterococcus faecalis-1, the Poulvac Escherichia coli vaccine, and their combination on growth parameters, intestinal microbial composition, immune response, and protection against challenge with the avian pathogen E. coli O78. The experimental groups were as follows: G1, basal diet; G2, basal diet and challenge with O78 at 28 days of growth; G3, basal diet, vaccination with Poulvac (1 and 15 days), and challenge with O78 at 28 days of growth; G4, basal diet, E. faecalis-1 supplementation for the first 3 days of growth, and challenge with O78 at 28 days of growth; G5, basal diet, E. faecalis-1 supplementation for the first 3 days of growth, vaccination with Poulvac (1 and 15 days), and challenge with O78 at 28 days of growth; G6, basal diet and E. faecalis-1 supplementation for the first 3 days of growth. The results showed that E. faecalis-1 in drinking water significantly improved the growth performance and immune response, increased the total Enterococcus counts, reduced the mortality, and decreased the visceral invasion by O78 in challenged broilers. While the effect of the Poulvac vaccine alone or with E. faecalis-1 was not significant compared with that of the E. faecalis-1 supplement, the vaccine improved the growth rate and decreased the mortality and visceral invasion by APEC O78 in challenged broilers. These results showed that E. faecalis-1 supplementation and routine vaccination with the Poulvac vaccine could improve the growth performance and immune response of broiler chickens and protect against challenge with APEC O78.

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References

  1. Havenstein GB, Ferket PR, Qureshi MA (2003) Growth, livability, and feed conversion of 1957 versus 2001 broilers when fed representative 1957 and 2001 broiler diets. Poult Sci 82(10):1500–1508

    PubMed  CAS  Google Scholar 

  2. Gaskins HR, Collier CT, Anderson DB (2002) Antibiotics as growth promotants: mode of action. Anim Biotechnol 13(1):29–42

    PubMed  CAS  Google Scholar 

  3. Williams RJ, Heymann DL (1998) Containment of antibiotic resistance. Science 279(5354):1153–1154

    PubMed  CAS  Google Scholar 

  4. Barton MD (2000) Antibiotic use in animal feed and its impact on human health. Nutr Res Rev 13(2):279–299

    PubMed  CAS  Google Scholar 

  5. Tarabees R, Gafar KM, El-Sayed MS, Shehata AA, Ahmed M (2018) Effects of dietary supplementation of probiotic mix and prebiotic on growth performance, cecal microbiota composition, and protection against Escherichia coli O78 in broiler chickens. Probiotics Antimicrob Proteins 11:981–989. https://doi.org/10.1007/s12602-018-9459-y

    Article  CAS  Google Scholar 

  6. FAO/WHO (2001) Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Report of a joint FAO/WHO expert consultation on evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria; FAO/WHO: Amerian Córdoba Park Hotel, Córdoba, Argentina, 2001, pp 1–34

  7. Cao GT, Zeng XF, Chen AG, Zhou L, Zhang L, Xiao YP, Yang CM (2013) Effects of a probiotic, Enterococcus faecium on growth performance, intestinal morphology, immune response, and cecal microflora in broiler chickens challenged with Escherichia coli K88. Poult Sci 92(11):2949–2955

    PubMed  CAS  Google Scholar 

  8. Higgins JP, Higgins SE, Wolfenden AD, Henderson SN, Torres-Rodriguez A, Vicente JL, Hargis BM, Tellez G (2010) Effect of lactic acid bacteria probiotic culture treatment timing on Salmonella Enteritidis in neonatal broilers. Poult Sci 89(2):243–247

    PubMed  CAS  Google Scholar 

  9. Farnell MB, Donoghue AM, de Los Santos FS, Blore PJ, Hargis BM, Tellez G, Donoghue DJ (2006) Upregulation of oxidative burst and degranulation in chicken heterophils stimulated with probiotic bacteria. Poult Sci 85(11):1900–1906

    PubMed  CAS  Google Scholar 

  10. Menconi A, Wolfenden AD, Shivaramaiah S, Terraes JC, Urbano T, Kuttel J, Kermer C, Hargis BM, Tellez G (2010) Effect of lactic acid and bacteria probiotic culture for the treatment of Salmonella enterica serovar Heidelberg in neonatal broiler chickens and turkey poults. Poult Sci 90:561–565

    Google Scholar 

  11. Pirgozliev V, Bravo D, Rose SP (2014) Rearing conditions influence nutrient availability of plant extracts supplemented diets when fed to broiler chickens. J Anim Physiol Anim Nutr (Berl) 98(4):667–671

    CAS  Google Scholar 

  12. Czerwinski J, Hojberg O, Smulikowska S, Engberg RM, Mieczkowska A (2010) Influence of dietary peas and organic acids and probiotic supplementation on performance and caecal microbial ecology of broiler chickens. Br Poult Sci 51(2):258–269

    PubMed  CAS  Google Scholar 

  13. Aalaei M, Khatibjoo A, Zaghari M, Taherpour K, Akbari Gharaei M, Soltani M (2018) Comparison of single- and multi-strain probiotics effects on broiler breeder performance, egg production, egg quality and hatchability. Br Poult Sci 59(5):531–538

    PubMed  CAS  Google Scholar 

  14. Adil S, Magray SN (2012) Impact and manipulation of gut microflora in poultry: a review. J Anim Vet Adv 11:873–877

    Google Scholar 

  15. Ognik K, Krauze M, Cholewińska E, Abramowicz K (2017) The effect of a probiotic containing Enterococcus faecium DSM 7134 on redox and biochemical parameters in chicken blood. Ann Anim Sci 17(4):1075–1088

    CAS  Google Scholar 

  16. Shehata A, Schrodl W, Neuhaus J, Kruger M (2013) Antagonistic effect of different bacteria on Clostridium botulinum types A, B, D and E in vitro. Vet Rec 172:74–78

    Google Scholar 

  17. Shehata AA, Tarabees R, Basiouni S, Gamil M, Kamal AS, Kruger M (2017) Phenotypic and genotypic characterization of bacteriocinogenic enterococci against Clostridium botulinum. Probiotics Antimicrob Proteins 9(2):182–188

    PubMed  CAS  Google Scholar 

  18. Shehata AA, Tarabees R, Basiouni S, Elsayed MS, Gaballah AA, Kruger M (2019) Effect of a potential probiotic candidate Enterococcus faecalis-1 on growth performance, intestinal microbiota and immune response of commercial broiler chickens. Probiotics Antimicrob Proteins. https://doi.org/10.1007/s12602-019-09557-2

  19. Guabiraba R, Schouler S (2015) Avian colibacillosis: still many black holes. FEMS Microbiol Lett 362(15):1–8

    Google Scholar 

  20. Ewers C, Antao EM, Diehl I, Philipp HC, Wieler LH (2009) Intestine and environment of the chicken as reservoirs for extraintestinal pathogenic Escherichia coli strains with zoonotic potential. Appl Environ Microbiol 75(1):184–192

    PubMed  CAS  Google Scholar 

  21. Huff WE, Huff GR, Rath NC, Balog JM, Donoghue AM (2002) Prevention of Escherichia coli infection in broiler chickens with a bacteriophage aerosol spray. Poult Sci 81(10):1486–1491

    PubMed  CAS  Google Scholar 

  22. Sadeyen JR, Wu Z, Davies H, van Diemen PM, Milicic A, La Ragione RM et al (2015) Immune responses associated with homologous protection conferred by commercial vaccines for control of avian pathogenic Escherichia coli in turkeys. Vet Res 46(5)

  23. Tarabees R, Elsayed MSA, Shawish R, Basiouni S, Shehata AA (2017) Isolation and characterization of Salmonella Enteritidis and Salmonella Typhimurium from chicken meat in Egypt. J Infect Dev Ctries 11(4):314–319

    PubMed  CAS  Google Scholar 

  24. Shehata AA, Klaus H, Pfalz T, Hafez MH, Kruger M (2016) Efficacy of cold fogging and oral herbal extracts on air quality and immune response of broilers. Aerobiologia (Bologna) 33:37–47

    Google Scholar 

  25. Ilstrup D (1990) Statistical methods in microbiology. Clin Microbiol Rev 3(3):219–226

    PubMed  PubMed Central  CAS  Google Scholar 

  26. Pires-dos-Santos T, Bisgaard M, Christensen H (2013) Genetic diversity and virulence profiles of Escherichia coli causing salpingitis and peritonitis in broiler breeders. Vet Microbiol 162(2–4):873–880

    PubMed  Google Scholar 

  27. Antao EM, Glodde S, Li G, Sharifi R, Homeier T, Laturnus C, Diehl I, Bethe A, Philipp HC, Preisinger R, Wieler LH, Ewers C (2008) The chicken as a natural model for extraintestinal infections caused by avian pathogenic Escherichia coli (APEC). Microb Pathog 45(5–6):361–369

    PubMed  CAS  Google Scholar 

  28. Mombarg M, Bouzoubaa K, Andrews S, Vanimisetti HB, Rodenberg J, Karaca K (2014) Safety and efficacy of an aroA-deleted live vaccine against avian colibacillosis in a multicentre field trial in broilers in Morocco. Avian Pathol 43(3):276–281

    PubMed  CAS  Google Scholar 

  29. Fernandes Filho T, Favaro C Jr, Ingberman M, Beirao BC, Inoue A, Gomes L, Caron LF (2013) Effect of spray Escherichia coli vaccine on the immunity of poultry. Avian Dis 57(3):671–676

    PubMed  Google Scholar 

  30. Lan RX, Lee SI, Kim IH (2017) Effects of Enterococcus faecium SLB 120 on growth performance, blood parameters, relative organ weight, breast muscle meat quality, excreta microbiota shedding, and noxious gas emission in broilers. Poult Sci 96(9):3246–3253

    PubMed  CAS  Google Scholar 

  31. Kim JS, Ingale SL, Kim YW, Kim KH, Sen S, Ryu MH, Lohakare JD, Kwon IK, Chae BJ (2012) Effect of supplementation of multi-microbe probiotic product on growth performance, apparent digestibility, cecal microbiota and small intestinal morphology of broilers. J Anim Physiol Anim Nutr (Berl) 96(4):618–626

    CAS  Google Scholar 

  32. Dada AC, Ahmad A, Usup G, Heng LY (2013) Speciation and antimicrobial resistance of enterococci isolated from recreational beaches in Malaysia. Environ Monit Assess 185(2):1583–1599

    PubMed  CAS  Google Scholar 

  33. Samli HE, Senkoylu N, Koc F, Kanter M, Agma A (2007) Effects of Enterococcus faecium and dried whey on broiler performance, gut histomorphology and intestinal microbiota. Arch Anim Nutr 61(1):42–49

    PubMed  Google Scholar 

  34. Abdel-Latif MA, Abd El-Hack ME, Swelum AA, Saadeldin IM, Elbestawy AR, Shewita RS, Ba-Awadh HA, Alowaimer AN, Abd El-Hamid HS (2018) Single and combined effects of Clostridium butyricum and Saccharomyces cerevisiae on growth indices, intestinal health, and immunity of broilers. Animals (Basel) 8(10)

  35. Patterson JA, Burkholder KM (2003) Application of prebiotics and probiotics in poultry production. Poult Sci 82(4):627–631

    PubMed  CAS  Google Scholar 

  36. La Ragione RM, Woodward MJ, Kumar M, Rodenberg J, Fan H, Wales AD, Karaca K (2013) Efficacy of a live attenuated Escherichia coli O78:K80 vaccine in chickens and turkeys. Avian Dis 57(2):273–279

    PubMed  Google Scholar 

  37. Galal HM, Tawfek AM, Abdrabou MI, Hessain AM, Alhaaji JH, Kabli SA, Elbehiry A, Alwarhi WK, Moussa IM (2018) Recent approaches for control of E. coli and respiratory complex in Middle East. Saudi J Biol Sci 25(7):1302–1307

    PubMed  PubMed Central  Google Scholar 

  38. Kim GB, Seo YM, Kim CH, Paik IK (2011) Effect of dietary prebiotic supplementation on the performance, intestinal microflora, and immune response of broilers. Poult Sci 90(1):75–82

    PubMed  CAS  Google Scholar 

  39. Gao P, Ma C, Sun Z, Wang L, Huang S, Su X et al (2017) Feed-additive probiotics accelerate yet antibiotics delay intestinal microbiota maturation in broiler chicken. Microbiome 5(91):1–14

    Google Scholar 

  40. Morishita TY, Aye PP, Harr BS, Cobb CW, Clifford JR (1997) Evaluation of an avian-specific probiotic to reduce the colonization and shedding of Campylobacter jejuni in broilers. Avian Dis 41:850–855

    PubMed  CAS  Google Scholar 

  41. Van Coillie E, Goris J, Cleenwerck I, Grijspeerdt K, Botteldoorn N, Van Immerseel F et al (2007) Identification of lactobacilli isolated from the cloaca and vagina of laying hens and characterization for potential use as probiotics to control Salmonella Enteritidis. J Appl Microbiol 102:1095–1106

    PubMed  Google Scholar 

  42. Timmerman HM, Veldman A, Van den Elsen E, Rombouts FM, Beynen AC (2006) Mortality and growth performance of broilers given drinking water supplemented with chicken-specific probiotics. Poult Sci 85:1383–1388

    PubMed  CAS  Google Scholar 

  43. Ferreira CL, Salminen S, Grezkowiak L, Brizuela M, Sanchez L, Carneiro H, Bonnet M (2011) Terminology concepts of probiotic and prebiotic and their role in human and animal health. Rev Salud Anim 33(3):137–146

    Google Scholar 

  44. Rhee KJ, Sethupathi P, Driks A, Lanning DK, Knight KL (2004) Role of commensal bacteria in development of gut-associated lymphoid tissues and preimmune antibody repertoire. J Immunol 172(2):1118–1124

    PubMed  CAS  Google Scholar 

  45. Luo J, Zheng A, Meng K, Chang W, Bai Y, Li K, Cai H, Liu G, Yao B (2013) Proteome changes in the intestinal mucosa of broiler (Gallus gallus) activated by probiotic Enterococcus faecium. J Proteome 91:226–241

    CAS  Google Scholar 

  46. Ateya AI, Arafat N, Saleh RM, Ghanem HM, Naguib D, Radwan HA, Elseady YY (2019) Intestinal gene expressions in broiler chickens infected with Escherichia coli and dietary supplemented with probiotic, acidifier and synbiotic. Vet Res Commun 43(2):131–142

    PubMed  Google Scholar 

  47. Genovese KJ, Anderson RC, Harvey RB, Nisbet DJ (2000) Competitive exclusion treatment reduces the mortality and fecal shedding associated with enterotoxigenic Escherichia coli infection in nursery-raised neonatal pigs. Can J Vet Res 64(4):204–207

    PubMed  PubMed Central  CAS  Google Scholar 

  48. Huff GR, Huff WE, Rath NC, Tellez G (2006) Limited treatment with beta-1,3/1,6-glucan improves production values of broiler chickens challenged with Escherichia coli. Poult Sci 85(4):613–618

    PubMed  CAS  Google Scholar 

  49. Attia YA, Ellakany HF, Abd El-Hamid AE, Fulvia B, Ghazaly SA (2012) Control of Salmonella Enteritidis infection in male layer chickens by acetic acid and/or prebiotics, probiotics and antibiotics. Arch Geflugelk 76:239–245

    CAS  Google Scholar 

  50. La Ragione RM, Casula G, Cutting SM, Woodward MJ (2001) Bacillus subtilis spores competitively exclude Escherichia coli O78 K80 in poultry. Vet Microbiol 79(2):133–142

    PubMed  Google Scholar 

  51. La Ragione RM, Narbad A, Gasson MJ, Woodward MJ (2004) In vivo characterization of Lactobacillus johnsonii FI9785 for use as a defined competitive exclusion agent against bacterial pathogens in poultry. Lett Appl Microbiol 38(3):197–205

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Bacteriology, Mycology, and Immunology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt

    Reda Tarabees & Mohamed S. El-Sayed

  2. Department of Birds and Rabbit Diseases, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Egypt

    Awad A. Shehata

  3. Faculty of Veterinary Medicine, Albrecht-Daniel-Thaer-Institute, University of Leipzig, Leipzig, Germany

    Awad A. Shehata

  4. Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, New Valley University, New Valley Governorate, Egypt

    Mohamed S. Diab

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  1. Reda Tarabees
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  2. Mohamed S. El-Sayed
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  3. Awad A. Shehata
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  4. Mohamed S. Diab
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Correspondence to Reda Tarabees.

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Tarabees, R., El-Sayed, M.S., Shehata, A.A. et al. Effects of the Probiotic Candidate E. faecalis-1, the Poulvac E. coli Vaccine, and their Combination on Growth Performance, Caecal Microbial Composition, Immune Response, and Protection against E. coli O78 Challenge in Broiler Chickens. Probiotics & Antimicro. Prot. 12, 860–872 (2020). https://doi.org/10.1007/s12602-019-09588-9

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