Abstract
The isolation of target-unrelated peptides (TUPs) through biopanning remains as a major problem of phage display selection experiments. These TUPs do not have any actual affinity toward targets of interest, which tend to be mistakenly identified as target-binding peptides. Therefore, an information portal for storing TUP data is urgently needed. Here, we present a TUP data bank (TUPDB), which is a comprehensive, manually curated database of approximately 73 experimentally verified TUPs and 1963 potential TUPs collected from TUPScan, the BDB database, and public research articles. The TUPScan tool has been integrated in TUPDB to facilitate TUP analysis. We believe that TUPDB can help identify and remove TUPs in future reports in the biopanning community. The database is of great importance to improving the quality of phage display-based epitope mapping and promoting the development of vaccines, diagnostics, and therapeutics. The TUPDB database is available at http://i.uestc.edu.cn/tupdb.
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Funding
This work was supported by the National Natural Science Foundation of China (grant number: 61901130, 61901129, and 62071099), the 2018 Talent Research Program of Guizhou University (grant numbers: (2018)54 and (2018)55), the Science and Technology Plan Project of Guizhou Province of China (grant numbers: (2018)5781, [2020]1Y407, and [2020] 1Y345), and the China Postdoctoral Science Foundation Grant (grant numbers: 2019M653369 and 2019M660236).
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B. H., S. Y., J. L., X. C., Q. Z., and H. C. developed the web interface of the database. B. H. collected and curated the data. H. C. and B. H. wrote the manuscript. B. H., J. H., and H. G. conceived the idea and coordinated the project.
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He, B., Yang, S., Long, J. et al. TUPDB: Target-Unrelated Peptide Data Bank. Interdiscip Sci Comput Life Sci 13, 426–432 (2021). https://doi.org/10.1007/s12539-021-00436-5
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DOI: https://doi.org/10.1007/s12539-021-00436-5