Abstract
A field study was carried out to investigate the effect of three Zn levels 0, 20 kg ZnSO4 ha−1 and 20 kg ZnSO4 ha−1+ foliar spray of 0.5 % ZnSO4 on superoxide dismutase activity, acid phosphatase activity and grain yield and a pot experiment to study the effect of zinc deficient and sufficient conditions on organic acid exudation. Increasing Zn levels was established as beneficial in improving the enzyme activities of genotypes. Combined foliar and soil application of Zn proved to be superior of all the treatments. Zinc application resulted in a maximum increment limit of 96.8 % in superoxide dismutase activity, 75.76 % in acid phosphatase activity, and a decrement limit of 88.57 % in oxalic acid exudation irrespective of stages and year of study. The increased enzyme activities had a positive impact on grain yield. As an average of all genotypes an improvement of 19.88 % in 2009 and 21.29 % in 2010 due to soil application while of 16.45 % in 2009 and 13.01 % in 2010 due to combined application was calculated for grain yield. There existed a variation among genotypes in showing responses towards zinc application and the genotypes UP 2584 and PBW 550 were found to be more responsive.
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References
Alloway BJ (2008) Zinc in soils and crop nutrition, 2nd edn. IZA, Brussels, 135 p
Alscher RG, Erturk N, Heath LS (2002) Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J Exp Bot 53(372):1331–1341
Asmar F, Gahoonia T, Nielsen N (1995) Barley genotypes differ in activity of soluble extracellular phosphatase and depletion of organic phosphorous in the rhizosphere soil. Plant Soil 172:117–122
Bharti K, Pandey N, Shankhdhar D, Srivastava PC, Shankhdhar SC (2013) Evaluation of some promising wheat genotypes (Triticum aestivum L.) at different zinc regimes for crop production. Cereal Res Commun. doi:10.1556/CRC.2013.0034
Cakmak I (2000) Role of zinc in protecting plant cells from reactive oxygen species. New Phytol 146:185–205
Coleman JE (1998) Zinc enzymes. Curr Opin Chem Biol 2:222–234
Frei M, Tanaka PJ, Wissuwa M (2010) Biochemical factors conferring shoot tolerance to oxidative stress in rice grown in low zinc soil. Funct Plant Biol 37:74–84
Fridivich I (1986) Superoxide dismutases. Adv Enzymol Relat Areas Mol Biol 58:61–97
Giannopolitis CN, Ries SK (1977) Superoxide dismutase occurrence in higher plants. Plant Physiol 59:309–314
Graham AW, McDonald GK (2001) Effect of zinc on photosynthesis and yield of wheat under heat stress. Proceedings of the 10th Australian Agronomy Conference 2001, Australian Society of Agronomy. Hobart, Tasmania, Australia. Available online at http://www.regional.org.au/au/asa/2001/2/c/graham.htm
Gunes A, Inal A (2008) Significance of intracellular and secreted acid phosphatase enzyme activities, and zinc and calcium interactions, on phosphorus efficiency in wheat, sunflower, chickpea, and lentil cultivars. Aust J Agric Res 59:339–347
Hacisalihoglu G, Kochian LV (2003) How do some plants tolerate low levels of soil zinc? Mechanisms of zinc efficiency in crop plants. New Phyt 159:341–350
Hoffland E, Wei C, Matthias W (2006) Organic anion exudation by Lowland Rice ( Oryza sativa L.) at zinc and phosphorus deficiency. Plant Soil 15(2):283
Kaya C, David H, Agneta B (2000) Phosphorus and acid phosphatase enzyme activity in leaves of tomato cultivars in relation to zinc supply. Comm Soil Sci Plant Anal 31(19–20):3239–3248
Marschner H (1995) Mineral nutrition of higher plants, 2nd edn. Academic Press, London, 889p
Narwal RP, Malik RS, Dahiya RR (2011) Addressing variations in status of a few nutritionally important micronutrients in wheat crop. www.zinccrops2011.org/…/2011_zinccrops2011
Pérez-Esteban J, Escolástico C, Moliner A, Masaguer A (2013) Chemical speciation and mobilization of copper and zinc in naturally contaminated mine soils with citric and tartaric acids. Chemosphere 90(2):276–283
Sadasivam S, Manickam A (1991) Biochemical methods, 2nd edn. New Age International (P) Limited, Coimbatore, 122p
Sillanpaa M (1990) Micronutrient assessment at country level: An international study, vol 63, FAO Soils Bulletin. FAO, Rome
Singh B, Singh BK (2011) Effect of reduced seed and applied zinc on zinc efficiency of wheat genotypes under zinc deficiency in nutrient solution culture. J Plant Nutr 34:449–464
Stoskopf NC (1985) Cereal grain crops. Reston Publishing Co., Inc., Reston
Szmigielska AM, VanRees KCJ, Cieslinski G, Huang PM (1996) Low molecular weight dicarboxylic acids in rhizosphere soil of durum wheat. J Agric Food Chem 44:1036–1040
Wang H, Jin JY (2005) Photosynthetic rate, chlorophyll fluorescence parameters, and lipid peroxidation of maize leaves as affected by zinc. Photosynthetica 43(4):591–596
Wang P, Zhou R, Cheng J, Bi S (2007) LC determination of trace short-chain organic acids in wheat root exudates under aluminum stress. Chromatographia 66:867–872
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The financial assistance provided by NAIP/ ICAR New Delhi, India is duly acknowledged.
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Bharti, K., Pandey, N., Shankhdhar, D. et al. Effect of different zinc levels on activity of superoxide dismutases & acid phosphatases and organic acid exudation on wheat genotypes. Physiol Mol Biol Plants 20, 41–48 (2014). https://doi.org/10.1007/s12298-013-0201-7
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DOI: https://doi.org/10.1007/s12298-013-0201-7